Name: mammary transplantation of stromal cells and carcinoma cells in c57bl/6j mice
Uploaded: 2011-08-12T12:00:00
Description: Watch this Scientific Journal Video about Mammary Transplantation of Stromal Cells and Carcinoma Cells in C57BL/6J Mice at JoVE.com

Bu proje üzerine NIH / NCI hibe sayısı R00 CA127357 ve Kansas Üniversitesi Kanser Merkezi Endowment tarafından finanse edildi.

1. C57BL/6J fareler İzolasyon ve donör kollajen çıkarma <ol><li> Onaylanmış IACUC yöntemler kullanılarak, normal olgun kadın C57BL/6J fareler Kurban. </li><li> Kuyrukları Hasat ve dokuları sterilize etmek için 45 dakika boyunca% 70 etanol içinde bekletin. Kuru kağıt mendil ile kuyrukları, alüminyum folyo ile sarın. -20 ° C kadar gerekli kuyrukları saklayın. </li><li> Steril bir ortamda böyle bir laminer akış kaputu gibi kuyrukları yerleştirin. Makas kullanarak, kuyruk kökünde çatlatmaya ve...

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Tümör progresyonu fibroblastların fonksiyonel katkı karsinom ilişkili fibroblastlar, artan tümör büyümesi ve invazivlik 5 iyi huylu meme epitel hücreleri ile birlikte nakledilen hangi transplantasyon modelleri ile kanıtlanmıştır . Konvansiyonel nakli yaklaşımlar, farklı genetik fare geçmişleri ya da farklı türlerin ortak nakli stromal ve epitelyal hücreleri SCID veya çıplak farelerin kullanılması yer var. Immün sistemi baskılanmış farelerde tümör spesifik antijen ve tümör hü...

<table border="1"><tbody><tr><th> Reaktifin Adı </th><th> Şirket </th><th> Katalog numarası </th><th> Yorumlar (isteğe bağlı) </th></tr><tr><td> C57BL/6N fareler </td><td> Harlan </td><td> N / A </td><td></td></tr><tr><td> MMTV-PyVmT transgenik farelerde </td><td> Jackson laboratuvarlar </td><td> 002374 </td><td></td></tr><tr><td> Fetal sığır serumu </td><td> Balıkçı </td><td> SH3039603PR </td><td></td></tr><tr><td> DMEM </td><td> VWR </td><td> 10000113873 </td><td></td></tr><tr><td> Penisilin / streptomisin </td><td> Balıkçı </td><td> MT-30-001 </td><td></td></tr><tr><td> amfoterisin </td><td> balıkçı </td><td> BP2645-20 </td><td></td></tr><tr><td> Amicon filtrasyon sütun ultracel 50k </td><td> Millipore </td><td> UFC905008 </td><td></td></tr><tr><td> Beckman TI rotor için tüpleri </td><td> Beckman </td><td> 355618 </td><td></td></tr><tr><td> Rat kuyruk kollajen </td><td> Balıkçı </td><td> CB 40.236 </td><td></td></tr><tr><td> 10x EBSS </td><td> Sigma Aldrich </td><td> E7510-100ML </td><td></td></tr><tr><td> Tripsin 1X, HBSS w / o Kalsiyum ve Magnezyum% 0.25 </td><td> Balıkçı </td><td> MT-25-050-CI </td><td></td></tr><tr><td> Asetik sirke asidi </td><td> Balıkçı </td><td> A491-212 </td><td></td></tr><tr><td> Coomasie mavi </td><td> Balıkçı </td><td> BP101 25 </td><td></td></tr><tr><td> Tripsin </td><td> Sigma Aldrich </td><td> T3924-100ml </td><td></td></tr><tr><td> Kollajenaz A </td><td> Sigma AldrichC0130-50 </td><td></td><td></td></tr><tr><td> hyalronidase </td><td> Sigma Aldrich </td><td> H3884 </td><td></td></tr><tr><td> DNaz </td><td> Sigma Aldrich </td><td> D5025 </td><td></td></tr><tr><td> Kaleidoscope Protein standardı </td><td> Biorad </td><td> 1610375 </td><td></td></tr><tr><td> Cam slaytlar </td><td> Balıkçı </td><td> 12545-78 </td><td></td></tr><tr><td> Cam lamelleri </td><td> VWR </td><td> 101400-042 </td><td></td></tr><tr><td> Vimentin antikor S-20 </td><td> Santa Cruz Biyoteknoloji </td><td> SC-7558 </td><td></td></tr><tr><td> α-düz kas aktin antikor </td><td> Abcam </td><td> ab5694 </td><td></td></tr><tr><td> CK14 antikor </td><td> Santa Cruz Biyoteknoloji </td><td> sc-53.253 </td><td></td></tr><tr><td> CK18 antikor </td><td> Abcam </td><td> ab668 </td><td></td></tr><tr><td> DAPI </td><td> Sigma Aldrich </td><td> D9542 </td><td></td></tr><tr><td> Anti-fare biotinlenmiş </td><td> Vektör laboratuvarlar </td><td> BA9200 </td><td> Fisher aracılığıyla dağıtılmaktadır </td></tr><tr><td> Anti-fare-alexa-568 </td><td> Invitrogen </td><td> A10037 </td><td></td></tr><tr><td> Anti-fare-alexa-488 </td><td> Invitrogen </td><td> A11001 </td><td></td></tr><tr><td> Streptavidin-alexa-488 </td><td> Invitrogen </td><td> S11226 </td><td></td></tr><tr><td> DAPI </td><td> Invitrogen </td><td> D21490 </td><td></td></tr><tr><td> Antifade Prolong </td><td> Invitrogen </td><td> P-36.930 </td><td></td></tr><tr><td> Cerrahi makas </td><td> Güzel Bilim Araçları </td><td> 91400-12 </td><td></td></tr><tr><td> Güzel bahar makas </td><td> Güzel Bilim Araçları </td><td> 15000-02 </td><td></td></tr><tr><td> Blunt forseps </td><td> Güzel Bilim Araçları </td><td> 11002-12 </td><td></td></tr><tr><td> # 5 ince forseps </td><td> Güzel Bilim Araçları </td><td> 11251-10 </td><td></td></tr><tr><td> Gut kromik sütür </td><td> Balıkçı </td><td> NC9326254 </td><td></td></tr><tr><td> Cam tamamen yeniden süspanse </td><td> Balıkçı </td><td> 22-042-815 </td><td></td></tr><tr><td> Etanol </td><td> Balıkçı </td><td> A406P 4 </td><td></td></tr><tr><td> betadin </td><td> balıkçı </td><td> NC9386574 </td><td></td></tr><tr><td> Yara klipleri </td><td> Balıkçı </td><td> 12032-07 </td><td></td></tr><tr><td> Yara elyaf </td><td> Balıkçı </td><td> 12031-07 </td><td></td></tr></tbody></table>

mammary transplantation of stromal cells and carcinoma cells in c57bl/6j mice

Stromal hücreler, fibroblastlar meme tümör progresyonu dahil olmak üzere etkisi özellikle farelerde meme bezi stromal hücreleri ve epitel hücrelerinin nakli yoluyla, fare modellerinin kullanımı ile belgelenmiştir. Şu nakli modelleri genellikle stromal hücreler ve epitel hücrelerinin farklı genetik geçmişleri nedeniyle immün sistemi baskılanmış farelerde içerir. Ekstrasellüler matrisler genelde tutarlı hücre-hücre etkileşimleri için iki farklı hücre tipleri gömmek, ancak Matrigel veya sıçan kuyruğu kollajen, immunojenik substratlar kullanımı dahil etmek için kullanılır. Immünkompromize farelerden elde edilen fonksiyonel T hücre eksikliği, ilaç geliştirme ve etkinliği üzerinde önemli etkileri, in vivo tümör progresyonu meme stromal hücreler doğru bir değerlendirme önler . Ayrıca, immün sistemi baskılanmış farelere, yüksek maliyetli sabit, özel bakım koşullarında ırk ve gerektirir. Bu engelleri aşmak için, biz orthotopically tutarlı tümör oluşumuna neden aynı genetik arka plan, stromal hücre ve epitel hücreleri farelere nakli için bir yaklaşım geliştirdik. Bu sistem, donör C57BL/6J fareler, normal karsinom ilişkili fibroblastlar, PyVmT meme kanseri hücreleri ve kollajen hasat içerir. Hücreler daha sonra kolajen gömülü ve kasık, dişi C57BL/6J farelerin meme bezleri nakledilen. PyVmT hücreleri tek başına Transplantasyon palpe edilebilir tümörler 30-40 gün transplantasyon sonrası oluşturur. 60 gün Son Nokta analizi fibroblastlar ile birlikte transplantasyon yalnız nakledilen PyVmT hücreleri ile karşılaştırıldığında meme tümör büyümesini artırır gösterir. C57BL/6J farelerin hücreleri ve matriks bu çalışmalarda kullanılan iken, hücre ve matriks izolasyonu ve transplantasyonu yaklaşım çok yönlülük gösteren farklı genetik kökenden farelere karşı uygulanabilir. Özetle, bu sistem stromal hücreleri ve epitel hücreleri arasındaki moleküler etkileşimler araştırmak için kullanılan olabilir ve immün sistemi baskılanmış fare modellerinde kritik kısıtlamaların üstesinden gelmektedir.

Watch this Scientific Journal Video about Mammary Transplantation of Stromal Cells and Carcinoma Cells in C57BL/6J Mice at JoVE.com

Mammary Transplantation of Stromal Cells and Carcinoma Cells in C57BL/6J Mice

Bu raporda, izole etmek için bir sistem ve fare meme bezi kültür donör hücreleri göstermek ve bu hücrelerin orthotopically nakli stromal analiz etmek için alıcı farelerde meme tümör gelişimi sırasında epitelyal etkileşimler.

C57BL/6J Fare Stromal Cells ve Karsinomu Hücreler Meme Nakli

The influence of stromal cells, including fibroblasts on mammary tumor progression has been well documented through the use of mouse models, in particular ...

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Medicine

Experimental Generation of Carcinoma-Associated Fibroblasts (CAFs) from Human Mammary Fibroblasts

Experimental Generation of Carcinoma-Associated Fibroblasts CAFs from Human Mammary Fibroblasts

İnsan Meme Fibroblastlar Karsinom İlişkili Fibroblastlar Deneysel Nesil (CAFs)

Carcinomas are complex tissues comprised of neoplastic cells and a non-cancerous compartment referred to as the 'stroma'. The stroma consists of ...

Human Internal Mammary Artery (IMA) Transplantation and Stenting: A Human Model to Study the Development of In-Stent Restenosis

Human Internal Mammary Artery IMA Transplantation and Stenting: A Human Model to Study the Development of In-Stent Restenosis

İnsan internal meme arter (İMA) Transplantasyon ve Stent: In-Stent Restenozis Geliştirme Eğitim Bir İnsan Modeli

Preclinical in vivo research models to investigate pathobiological and pathophysiological processes in the development of intimal hyperplasia after vessel ...

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods

İnsan Diş Pulp Kök Hücre İzolasyonu, Karakterizasyonu ve Karşılaştırmalı Farklılaşma İki Farklı Yöntemler Kullanılarak Daimi Dişler türetilen

Developing wisdom teeth are easy-accessible source of stem cells during the adulthood which could be obtained by routine orthodontic treatments. Human ...

Generation of Subcutaneous and Intrahepatic Human Hepatocellular Carcinoma Xenografts in Immunodeficient Mice

Bağışıklık yetersizliği olan farelere subkutan olarak ve intrahepatik insan hepatoselüler karsinoma ksenograftlarının Üretimi

In  vivo experimental models of hepatocellular carcinoma (HCC) that recapitulate  the human disease provide a valuable platform for research into disease  ...

Coculture System with an Organotypic Brain Slice and 3D Spheroid of Carcinoma Cells

Bir Organotipik Beyin Dilim ve kanser hücrelerinin 3D Sferoid ile Coculture Sistemi

Patients with cerebral metastasis of carcinomas have a poor prognosis. However, the process at the metastatic site has barely been investigated, in ...

Modeling Spontaneous Metastatic Renal Cell Carcinoma (mRCC) in Mice Following Nephrectomy

Modeling Spontaneous Metastatic Renal Cell Carcinoma mRCC in Mice Following Nephrectomy

Farelerde spontan metastatik renal hücreli karsinom (MRCC) modelleme ardından nefrektomi

One of the key challenges to improved testing of new experimental therapeutics in renal cell carcinoma (RCC) is the development of models that faithfully ...

Orthotopic Injection of Breast Cancer Cells into the Mammary Fat Pad of Mice to Study Tumor Growth.

Farelerin Meme Fat Pad içine Meme Kanseri Hücreleri Ortotopik Enjeksiyon Tümör Büyüme Eğitim için.

Breast cancer growth can be studied in mice using a plethora of models. Genetic manipulation of breast cancer cells may provide insights into the ...

Evaluation of Stem Cell Properties in Human Ovarian Carcinoma Cells Using Multi and Single Cell-based Spheres Assays

Çoklu ve tek hücre bazlı küreler tahliller kullanılarak, insan yumurtalık karsinoma hücrelerinde Kök Hücre Özelliklerinin Değerlendirilmesi

Years of research indicates that ovarian cancers harbor a heterogeneous mixture of cells including a subpopulation of so-called &#8220;cancer stem ...

Studying Pancreatic Cancer Stem Cell Characteristics for Developing New Treatment Strategies

Pankreas Kanseri Yeni Tedavi Stratejileri Geliştirme Hücre Özellikleri Kök incelenmesi

Pancreatic ductal adenocarcinoma (PDAC) contains a subset of exclusively tumorigenic cancer stem cells (CSCs) which have been shown to drive tumor ...

Heterotopic Cervical Heart Transplantation in Mice

Farelerde Heterotopik Servikal Kalp Nakli

The heterotopic cervical heart transplantation in mice is a valuable tool in transplant and cardiovascular research. The cuff technique greatly simplifies ...