Name: microsurgical venous pouch arterial-bifurcation aneurysms in the rabbit model: technical aspects
Uploaded: 2011-05-11T13:00:00
Description: Watch this Scientific Journal Video about Microsurgical Venous Pouch Arterial-Bifurcation Aneurysms in the Rabbit Model: Technical Aspects at JoVE.com

Diese Studie wurde durch die &quot;Medizinisch-Wissenschaftlichen Fonds des Bürgermeisters von der Stadt Wien&quot; und Ethicon Biosurgery, Inc. New Jersey, USA unterstützt. Die Autoren bedanken sich bei Professor Heber Ferraz-Leite, Direktor des &quot;European Workshop on Mikrochirurgie und Cerebral Revascularization&quot; an der Medizinischen Universität Wien, Österreich, für seine wertvolle mikrochirurgischen Lehre.

1. Positionierung und Vorbereitung des Tieres Die Experimente wurden von den zuständigen lokalen Ethikkommission genehmigt und durchgeführt nach den FELASA Richtlinien. <ol><li> Starten Sie eine Vollnarkose des weiblichen New Zealand White Kaninchen (2,5-3,5 kg) mit intramuskulären Injektion von Ketamin (30mg/kg) und Xylazin (6mg/kg) und weiter nach mechanischer Intubation (Tubus Durchmesser 4mm, Länge 18mm) mit Gas Anästhesie ( Isofluran) unter EKG-Überwachung. Führen Sie Schmerzen...

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	<li>Molyneux, A., Kerr, R., Yu, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=International+subarachnoid+aneurysm+trial+(ISAT)+of+neurosurgical+clipping+versus+endovascular+coiling+in+2143+patients+with+ruptured+intracranial+aneurysms:+a+randomised+comparison+of+effects+on+survival,+dependency,+seizures,+rebleeding,+subgroups,+and+aneurysm+occlusion.">International subarachnoid aneurysm trial (ISAT) of neurosurgical clipping versus endovascular coiling in 2143 patients with ruptured intracranial aneurysms: a randomised comparison of effects on survival, dependency, seizures, rebleeding, subgroups, and aneurysm occlusion.</a> Lancet. 366, 809-817 (2005).</li><li>Mitchell, P., Kerr, R., Mendelow, A., Molyneux, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Could+late+rebleeding+overturn+the+superiority+of+cranial+aneurysm+coil+embolization+over+clip+ligation+seen+in+the+International+Subarachnoid+Aneurysm+Trial.">Could late rebleeding overturn the superiority of cranial aneurysm coil embolization over clip ligation seen in the International Subarachnoid Aneurysm Trial.</a> J Neurosur. 108, 437-442 (2008).</li><li>Bavinzski, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Histopathological+findings+in+experimental+aneurysms+embolized+with+conventional+and+thrombogenic/antithrombolytic+Guglielmi+coils.">Histopathological findings in experimental aneurysms embolized with conventional and thrombogenic/antithrombolytic Guglielmi coils.</a> Minim Invasive Neurosurg. 42, 167-174 (1999).</li><li>Bocher-Schwarz, H. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Histological+findings+in+coil-packed+experimental+aneurysms+3+months+after+embolization.">Histological findings in coil-packed experimental aneurysms 3 months after embolization.</a> Neurosurgery. 50, 379-384 (2002).</li><li>Sherif, C., Plenk, H., Grossschmidt, K., Kanz, F., Bavinzski, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Computer-assisted+quantification+of+occlusion+and+coil+densities+on+angiographic+and+histological+images+of+experimental+aneurysms.">Computer-assisted quantification of occlusion and coil densities on angiographic and histological images of experimental aneurysms.</a> Neurosurgery. 58, 559-566 (2006).</li><li>Massoud, T., Guglielmi, G., Ji, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Experiemental+saccular+aneurysms.+1.+Review+of+surgically+constructed+models+and+their+laboratory+applications.">Experiemental saccular aneurysms. 1. Review of surgically constructed models and their laboratory applications.</a> Neurorad. 36, 537-546 (1994).</li><li>Anidjar, S., Salzmann, J., Gentric, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Elastase+induced+experimental+aneurysms+in+rats.">Elastase induced experimental aneurysms in rats.</a> Circulation. 82, 973-981 (1990).</li><li>Wakhloo, A., Schellhammer, F., de Vries, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Self-expanding+and+balloon-expandable+stents+in+the+treatment+of+carotid+aneurysms:+an+experimental+study+in+a+canine+model.">Self-expanding and balloon-expandable stents in the treatment of carotid aneurysms: an experimental study in a canine model.</a> AJNR. 15, 493-502 (1994).</li><li>Dai, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Histopathologic+and+immunohistochemical+comparison+of+human,+rabbit,+and+swine+aneurysms+embolized+with+platinum+coils.">Histopathologic and immunohistochemical comparison of human, rabbit, and swine aneurysms embolized with platinum coils.</a> AJNR Am J Neuroradiol. 26, 2560-2568 (2005).</li><li>Shin, Y. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Creation+of+four+experimental+aneurysms+with+different+hemodynamics+in+one+dog.">Creation of four experimental aneurysms with different hemodynamics in one dog.</a> AJNR. 26, 1764-1767 (2005).</li><li>Abruzzo, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Histologic+and+morphologic+comparison+of+experimental+aneurysms+with+human+intracranial+aneurysms.">Histologic and morphologic comparison of experimental aneurysms with human intracranial aneurysms.</a> AJNR Am J Neuroradiol. 19, 1309-1314 (1998).</li><li>Bavinzski, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Experimental+bifurcation+aneurysm:+a+model+for+in+vivo+evaluation+of+endovascular+techniques.">Experimental bifurcation aneurysm: a model for in vivo evaluation of endovascular techniques.</a> Minim Invasive Neurosurg. 41, 129-132 (1998).</li><li>Spetzger, U. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Microsurgically+produced+bifurcation+aneurysms+in+a+rabbit+model+for+endovascular+coil+embolization.">Microsurgically produced bifurcation aneurysms in a rabbit model for endovascular coil embolization.</a> J Neurosurg. 85, 488-495 (1996).</li><li>Sherif, C., Marbacher, S., Erhardt, S., Fandino, J., J, . <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Improved+microsurgical+creation+of+venous-pouch+arterial-bifurcation+aneurysms+in+rabbits.">Improved microsurgical creation of venous-pouch arterial-bifurcation aneurysms in rabbits.</a> AJNR. , .</li><li>Marbacher, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Complex+Bi-Lobular,+Bi-Saccular,+and+Broad-Necked+Microsurgical+Aneurysms+Formation+in+the+Rabbit+Bifurcation+Model+for+the+Study+of+Upcoming+Endovascular+Techniques.">Complex Bi-Lobular, Bi-Saccular, and Broad-Necked Microsurgical Aneurysms Formation in the Rabbit Bifurcation Model for the Study of Upcoming Endovascular Techniques.</a> AJNR. , .</li><li>Sherif, C., Plenk, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Quantitative+angiographic+and+histopathologic+evaluation+of+experimental+aneurysms.">Quantitative angiographic and histopathologic evaluation of experimental aneurysms.</a> AJNR. , .</li><li>Grunwald, I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Anticoagulation+regimes+and+their+influence+on+the+occlusion+rate+of+aneurysms:+an+experimental+study+in+rabbits.">Anticoagulation regimes and their influence on the occlusion rate of aneurysms: an experimental study in rabbits.</a> Neurosurg. 57, 1048-1054 (2005).</li><li>Forrest, M. D., O'Reilly, G. V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Production+of+experimental+aneurysms+at+a+surgically+created+arterial+bifurcation.">Production of experimental aneurysms at a surgically created arterial bifurcation.</a> AJNR Am J Neuroradiol. 10, 400-402 (1989).</li></ol>

Die Autoren empfehlen, rücksichtsvoll folgende kritische chirurgische wichtige Schritte, um gute Ergebnisse und Patentanmeldungen Aneurysmen zu erreichen: <ol><li> Eine sorgfältige Präparation ein langes Segment des linken CCA für die einfache Anastomose ohne Zerstörung von Nerven und kleinen Gefäßen. </li><li> Vorsichtiges Entfernen der hoch thrombogene periadventitial Bindegewebe vor der Gründung der Anastomose. </li><li> Erstellung einer spannungslosen Anastomose. </li><li> Weniger Nähte: Die vorgeschlag...

<table border="1"><tbody><tr><th> Name des Reagenzes </th><th> Firma </th><th> Katalog-Nummer </th><th> Kommentare </th></tr><tr><td> Vicryl4-0 polyfile wiederherstellbar Nähten </td><td> Ethicon Inc </td><td> J386H </td><td></td></tr><tr><td> Ethilon 10-0 monofil nicht resorbierbare Fäden </td><td> Ethicon Inc </td><td> 2814 </td><td> Kegel Punkt Nadel </td></tr><tr><td> Evicel BioGlue </td><td> Ethicon Biosurgery Inc. </td><td> 3901 </td><td></td></tr><tr><td> Fentanyl Hautpflaster 12,5 ug / h </td><td></td><td></td><td> Jede GENERICON </td></tr><tr><td> Heparin </td><td></td><td></td><td> Jede GENERICON </td></tr><tr><td> 0,9% Saline </td><td></td><td></td><td> Jede GENERICON </td></tr><tr><td> 4% Papaverin HCl </td><td></td><td></td><td> Jede GENERICON </td></tr><tr><td> Neomycinsulfat 5 mg / ml </td><td></td><td></td><td> Jede GENERICON </td></tr><tr><td> Ketamin 50mg/ml </td><td></td><td></td><td> Jede GENERICON </td></tr><tr><td> Xylazin 20mg/ml </td><td></td><td></td><td> Jede GENERICON </td></tr></tbody></table>

microsurgical venous pouch arterial-bifurcation aneurysms in the rabbit model: technical aspects

Für geplatzten menschlichen zerebralen Aneurysmen endovaskuläre Embolisation geworden eine gleichwertige Alternative zu Aneurysma Clipping. 1 Allerdings großen klinischen Studien haben enttäuschende Langzeitergebnisse mit inakzeptablen hohen Aneurysma Rekanalisation und verzögerte Aneurysmaruptur gezeigt. 2 Um diese Probleme zu überwinden, tierexperimentelle Studien sind für die Entwicklung besserer endovaskulären Geräten von entscheidender Bedeutung. 3-5 Mehrere Tiermodelle in Ratten, Kaninchen, Hunden und Schweinen zur Verfügung. 6-8 Vergleiche der verschiedenen Tiermodellen zeigten die Überlegenheit der Kaninchen-Modell im Hinblick auf die Hämodynamik und die Vergleichbarkeit des Gerinnungssystems und Wirtschaftlichkeit. 9-11 Das venöse Beutel arteriellen Bifurkation Modell in Kaninchen wird von einer venösen Beutel in einer künstlich geschaffenen wahren Bifurkation beider Arteria carotis communis (CCA) vernäht gebildet. Der Hauptvorteil dieses Modells sind wahre bifurcational Hämodynamik. 12 Die größten Nachteile sind die hohen sofar mikrochirurgischen technischen Anforderungen und eine hohe Morbidität und Mortalität von bis zu 50%. 13 Diese Beschränkungen haben in seltener Verwendung dieses Aneurysma-Modell in der letzten Folge Jahren. Diese Mängel mit verbesserten chirurgischen Verfahren zu überwinden und modifiziert werden peri-und postoperative analgetische Management und Antikoagulation. 14-16 Unsere Techniken in dieser Veröffentlichung zeigen die optimierte Technik zur mikrochirurgischen Schaffung arteriellen Bifurkation Aneurysmen.

Watch this Scientific Journal Video about Microsurgical Venous Pouch Arterial-Bifurcation Aneurysms in the Rabbit Model: Technical Aspects at JoVE.com

Microsurgical Venous Pouch Arterial-Bifurcation Aneurysms in the Rabbit Model: Technical Aspects

Eine optimierte Technik zur mikrochirurgischen Schaffung arteriellen Bifurkation Aneurysmen imitiert Bifurkation menschlichen zerebralen Aneurysmen beschrieben. Eine venöse Beutel wird in eine künstlich geschaffene wahre Bifurkation beider Arteria carotis communis vernäht. Erleichterte mikrochirurgische Techniken und aggressive postoperative Antikoagulation und Analgesie zu minimieren Morbidität und hohe Aneurysma Offenheitsraten führen.

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