Name: methods for ecg evaluation of indicators of cardiac risk, and susceptibility to aconitine-induced arrhythmias in rats following status epilepticus
Uploaded: 2011-04-05T13:00:00
Description: Watch this Scientific Journal Video about Methods for ECG Evaluation of Indicators of Cardiac Risk, and Susceptibility to Aconitine-induced Arrhythmias in Rats Following Status Epilepticus at JoVE.com

这项研究是由授予美国公民在癫痫（治愈）研究的SLB支持。

<p class="jove_title"&gt; 1。材料构造</p&gt;<ol&gt;<li颈内静脉导管斜面的一端，从PE - 50聚乙烯管件（100毫米）建造，然后用充满肝素盐水（肝素50 U / mL生理盐水）。</li&gt;<li&gt;心电图记录电极的构造从两个绝缘的银线（30AWG）的100毫米的长度。被剥离的两条电线的一端焊接到一个microconnector，是扭曲形成了第三条线是地面和绝缘。五毫米的绝缘层剥去电线的末端，并丢弃。</li&gt;</ol&gt;<p class="jove_title"&gt; 2。植入颈静脉导?...

<ol>
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所描述的过程的两个方面是至关重要的。首先，乌头管理率心必须相当于整个动物。这需要安置的颈内静脉导管提示心一致，并仔细调整输液速度。必须平等地以适当的评价室性心律失常的相对改变易感性的发病率乌头交付心中。如果乌头交付变化，则心律失常的延迟可能是由于药物浓度的差异，而不是在心脏功能的改变。其次，编制和心电图记录电极放置必须清楚可辨别的P，QRS波，T波与产量?...

methods for ecg evaluation of indicators of cardiac risk, and susceptibility to aconitine-induced arrhythmias in rats following status epilepticus

在一些病理条件下，致命的心律失常死亡率。建立一个非侵入性，容易获得的心电图（ECG）获得多项参数，验证患心肌病的患者的预后指标的心脏风险。心率增快，心率变异性（HRV）下降，增加的持续时间和心室电活动的变化（QT间期），都表明增强心脏的风险1-4。在动物模型中，它是有价值的，比较这些心电图派生变量和药敏实验诱发心律失常。静脉输液心律失常剂乌头已被广泛用于评估在一系列的实验条件下，包括5抑郁症和高血压6动物模型的心律失常易感性， 以下 7锻炼和接触到的空气污染物 8，以及确定的抗心律失常9,10药理剂的疗效 。 应该指出，在人类QT离散度，是衡量整个全套的信息从一个标准的12导联心电图QT间隔变化。因此，在本议定书中所描述的大鼠从2导联心电图QT分散的措施，是从人类的心电图记录计算，不同。这代表在翻译从人类临床用药的啮齿类动物中获得的数据的限制。 癫痫持续状态（SE）是一个单一的扣押或一系列不断反复发作，持久的死亡率超过30 分钟11,12 11,12，结果20％的案件 13 。许多人的生存本身，而是死于内30 天 14,15 。这个延迟死亡率（S）的机制尚不完全清楚。有人曾建议，致命的室性心律失常的贡献很多，这些死亡的 14-17 。除了 ​​向东南，经历自发性反复发作，如癫痫，的患者在癫痫（SUDEP）18有关的过早突然和意外死亡的风险。与SE，SUDEP调解的确切机制尚不清楚。有人曾提出，心室的异常，并导致心律失常作出了重大贡献 18-22 。 调查扣押相关的心源性死亡，和心脏疗法的疗效机制，有必要获得心源性风险指标和评估癫痫动物模型23-25 ​​心律失常的易感性。在这里，我们描述了心电图电极植入实验室只Sprague - Dawley鼠（褐家鼠）的方法，SE，收集和分析的心电图记录，四乌头输液期间和诱导的心律失常。 这些程序可以用来直接确定之间的关系心电图衍生的措施，以室性心律失常的心电活动和易感性，癫痫的大鼠模型，或与心源性猝死的风险增加相关的任何病理。

Watch this Scientific Journal Video about Methods for ECG Evaluation of Indicators of Cardiac Risk, and Susceptibility to Aconitine-induced Arrhythmias in Rats Following Status Epilepticus at JoVE.com

Methods for ECG Evaluation of Indicators of Cardiac Risk, and Susceptibility to Aconitine-induced Arrhythmias in Rats Following Status Epilepticus

测量心电图（ECG）的心电活动，和心脏危险因素和心律失常的易感性在大鼠癫痫持续状态（SE）的分析技术进行了阐述。

心电图心脏风险评价指标和乌头碱诱发大鼠心律失常的易感性继癫痫持续状态的方法

Lethal cardiac arrhythmias contribute to mortality in a number of pathological conditions. Several parameters obtained from a 
non-invasive, easily ...

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