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DOI :
10.3791/2730-v
•
13:40 min
July 7th, 2011
Chapters
0:05
Title
2:16
Preparation of Stock Solutions
3:03
Gene Silencing in HeLa Cells
5:39
Complementation of HeLa Cells
12:58
Conclusion
7:07
Live Cell Calcium Imaging
9:58
Representative Calcium Imaging Results
内质网在蛋白质生物合成和钙稳态的关键作用。我们已经建立了一个实验系统,使我们能够解决的Ca2 +泄漏通道的作用,并表征其公认的监管机制。该系统涉及的siRNA介导的基因沉默和活细胞内Ca2 +成像。
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