Name: mouse complete stasis model of inferior vena cava thrombosis
Uploaded: 2011-06-15T14:00:00
Description: Watch this Scientific Journal Video about Mouse Complete Stasis Model of Inferior Vena Cava Thrombosis at JoVE.com

1。鼠标麻醉程序<ol><li>动物从它们的笼子中被删除，和放置在手术前麻醉诱导在5％异氟醚和100％的氧气室气体感应，在每分钟0.5升（蒸发器控制）的流量。 </li><li>小鼠镇静麻醉诱导室，室拆除，剃腹腹部与电动快船，或分庭内保持，如果使用一毛脱毛（1分）。 </li><li>虽然仍在镇静，他们权衡，眼睛用无菌眼药膏润滑，然后放置在一个温暖的循环水加热装置，在全身麻醉下背recumbancy，用鼻锥?...

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	<li>Naess, I. A., Christiansen, S. C., Romundstad, P., Cannegieter, S. C., Rosendaal, F. R., Hammerstrom, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Incidence+and+mortality+of+venous+thrombosis:+a+population-based+study.">Incidence and mortality of venous thrombosis: a population-based study.</a> J Thromb Haemost. 5, 692-699 (2007).</li><li>White, R. H., Keenan, C. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effects+of+race+and+ethnicity+on+the+incidence+of+venous+thromboembolism.">Effects of race and ethnicity on the incidence of venous thromboembolism.</a> Thromb Res. 123, S11-S17 (2009).</li><li>Heit, J. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+epidemiology+of+venous+thromboembolism+in+the+community.">The epidemiology of venous thromboembolism in the community.</a> Arterioscler Thromb Vasc Biol. 28, 370-372 (2008).</li><li>Henke, P. K., Varga, A., De, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Deep+vein+thrombosis+resolution+is+modulated+by+monocyte+CXCR2-mediated+activity+in+a+mouse+model.">Deep vein thrombosis resolution is modulated by monocyte CXCR2-mediated activity in a mouse model.</a> Arterioscler Thromb Vasc Biol. 24, 1130-1137 (2004).</li><li>McDonald, A. P., Meier, T. R., Hawley, A. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Aging+is+associated+with+impaired+thrombus+resolution+in+a+mouse+model+of+stasis+induced+thrombosis.">Aging is associated with impaired thrombus resolution in a mouse model of stasis induced thrombosis.</a> Thromb Res. 125, 72-78 (2010).</li><li>Myers, D., Farris, D., Hawley, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Selectins+influence+thrombosis+in+a+mouse+model+of+experimental+deep+venous+thrombosis.">Selectins influence thrombosis in a mouse model of experimental deep venous thrombosis.</a> J Surg Res. 108, 212-221 (2002).</li><li>Myers, D. D., Hawley, A. E., Farris, D. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=P-selectin+and+leukocyte+microparticles+are+associated+with+venous+thrombogenesis.">P-selectin and leukocyte microparticles are associated with venous thrombogenesis.</a> J Vasc Surg. 38, 1075-1089 (2003).</li><li>Myers, D. D. J. r., Rectenwald, J. E., Bedard, P. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Decreased+venous+thrombosis+with+an+oral+inhibitor+of+P+selectin.">Decreased venous thrombosis with an oral inhibitor of P selectin.</a> J Vasc Surg. 42, 329-336 (2005).</li></ol>

鼠标完成瘀下腔静脉血栓模型上可以执行多个不同年龄的小鼠品系。该模型是最适合小鼠至少20克的重量。这种静脉血栓形成的动物模型，使调查研究静脉壁和闭塞的静脉血栓炎症之间的相互作用。从我们的经验，我们发现，每10个或更多组动物都需要获得重量血栓，静脉壁炎性细胞计数，静脉壁蛋白，静脉壁的基因表达，和全血或血浆活动分析组之间统计学意义检测。

<table border="1"><tbody><tr><td> 用品和设备 </td></tr><tr><td>异氟醚气体</td></tr><tr><td>普理灵7-0缝合</td></tr><tr><td>薇乔5-0缝合</td></tr><tr><td>皮胶</td></tr><tr><td>眼润滑油</td></tr><tr><td> Nolvasan解决方案或抑菌的解决方案</td></tr><tr><td>体视显微镜范围</td></tr><tr><td> MediCapture </td></tr><tr><td>棉花喷枪</td></tr><tr><td> 2x2的纱布</td></tr><tr><td>加热垫</td></tr><tr><td>麻醉机</td></tr><tr><td>玻璃珠仪器消毒柜</td></tr><tr><td>微型手术器械</td></tr><tr><td>腹部限位</td></tr></tbody></table>

mouse complete stasis model of inferior vena cava thrombosis

静脉血栓栓塞（VTE）包括深静脉血栓形成（DVT）和肺栓塞（PE）。在美国（美国），高发病率和死亡率的利率使VTE的一个严重的健康问题 1-2 。心脏病和中风后，静脉血栓栓塞是第三大最常见的血管疾病 3 。仅在美国，就有影响，每年估计有900000人，与30万死亡发生每年 3 。一个可靠在体内动物模型，以研究这种疾病的机制是必要的。 使用鼠标完成瘀下腔静脉血栓模型的优点是几个。在小鼠模型，让有限的可用性测试剂的极少量的管理，大大降低了成本。最有前途的是与基因敲除，让特定的炎症和凝血因子功能划定小鼠的潜力。目前的分子检测允许静脉壁血栓，全血和血浆用于检测的定量。然而，这种模式的一个重大问题，涉及手术的大小的限制，血管脆性。此外，由于下腔静脉的小样品的重量（平均0.005克），它是必要的，以增加动物数量进行准确的统计分析，组织，血栓和血液检测，如实时聚合酶链反应（RT - PCR检测），免疫印迹，酶联免疫吸附试验（ELISA），酶谱法，静脉壁和血栓细胞分析，全血和血浆检测4-8。 血瘀模型的主要缺点是，血流量不足，抑制全身治疗药物的管理上的血栓和静脉壁的最大效应。

Watch this Scientific Journal Video about Mouse Complete Stasis Model of Inferior Vena Cava Thrombosis at JoVE.com

Mouse Complete Stasis Model of Inferior Vena Cava Thrombosis

鼠标完成瘀下腔静脉血栓模型的静脉壁组织和血栓产生的可量化的数额。它已被证明有用的评估之间的静脉壁和闭塞的血栓，并在评估急性慢性发炎恶化的互动。

鼠标完成血瘀模型的下腔静脉血栓

Venous thromboembolism (VTE) includes both deep vein thrombosis (DVT) and pulmonary embolism (PE).  In the United States (U.S.), the high morbidity and ...

Research

JoVE Journal

Medicine

Electrolytic Inferior Vena Cava Model (EIM) of Venous Thrombosis

Electrolytic Inferior Vena Cava Model EIM of Venous Thrombosis

电解下腔静脉静脉血栓形成的模型（EIM）

Animal models serve a vital role in deep venous thrombosis (DVT) research in order to study thrombus formation, thrombus resolution and to test potential ...

科研

医学

Quantitative Analysis and Characterization of Atherosclerotic Lesions in the Murine Aortic Sinus

Atherosclerosis is a disease of the large arteries and a major underlying cause of myocardial infarction and stroke. Several different mouse models have ...

Implantation of Inferior Vena Cava Interposition Graft in Mouse Model

下腔静脉间置移植物在小鼠模型中植入

Biodegradable scaffolds seeded with bone marrow mononuclear cells (BMCs) are often used for reconstructive surgery to treat congenital cardiac anomalies. ...

Orthotopic Injection of Breast Cancer Cells into the Mammary Fat Pad of Mice to Study Tumor Growth.

注射原位乳腺癌细胞到小鼠乳腺脂肪垫来研究肿瘤的生长。

Breast cancer growth can be studied in mice using a plethora of models. Genetic manipulation of breast cancer cells may provide insights into the ...

Ferric Chloride-induced Thrombosis Mouse Model on Carotid Artery and Mesentery Vessel

三氯化铁诱发血栓形成小鼠模型上的颈动脉和肠系膜血管

Severe thrombosis and its ischemic consequences such as myocardial infarction, pulmonary embolism and stroke are major worldwide health issues. The ferric ...

Ferric Chloride-induced Murine Thrombosis Models

三氯化铁诱导小鼠模型的血栓形成

Arterial thrombosis (blood clot) is a common complication of many systemic diseases associated with chronic inflammation, including atherosclerosis, ...

Patch Angioplasty in the Rat Aorta or Inferior Vena Cava

血管补片在大鼠主动脉和下腔静脉

Pericardial patches are commonly used in vascular surgery to close vessels. To facilitate studies of the neointimal hyperplasia that forms on the patch, ...

A Rat Model of Orthotopic Liver Transplantation Using a Novel Magnetic Anastomosis Technique for Suprahepatic Vena Cava Reconstruction

应用新型磁吻合技术 Suprahepatic 腔静脉重建大鼠原位肝移植模型的研究

The rat model of orthotopic liver transplantation (OLT) is essential for transplant research. It is a very sophisticated animal model and requires a steep ...

Ferric Chloride-induced Canine Carotid Artery Thrombosis: A Large Animal Model of Vascular Injury

氯化铁诱导的犬颈动脉血栓形成: 一种大的血管损伤动物模型

Occlusive arterial thrombosis leading to cerebral ischemic stroke and myocardial infarction contributes to ~13 million deaths every year globally. Here, ...

Isometric Contractility Measurement of the Mouse Mesenteric Artery Using Wire Myography

用线 Myography 测定小鼠肠系膜动脉的等轴收缩力

The wire myograph technique is used to assess the contractility of vascular smooth muscles in response to depolarization, GPCR agonists/inhibitors and ...