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从石蜡包埋材料的遗传和表观遗传分析的DNA提取

DOI :

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13:32 min

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March 26th, 2011

March 26th, 2011

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55,059 Views

Larissa A. Pikor^1,2*, Katey S. S. Enfield^1,2*, Heryet Cameron³, Wan L. Lam^1,2,4

¹Department of Integrative Oncology, BC Cancer Research Centre, ²Interdisciplinary Oncology Program, University of British Columbia - UBC, ³Photography/Video Production, Multi-Media Services, BC Cancer Agency, ⁴Department of Pathology and Laboratory Medicine, University of British Columbia - UBC

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本视频演示了从福尔马林固定石蜡包埋的材料DNA提取的协议。这是一个多天的过程中，水化组织切片用二甲苯deparaffinized，乙醇和蛋白酶K处理，净化和隔离随后的特定基因或全基因组分析的DNA。

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