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DOI :
10.3791/2779-v
•
10:27 min
July 18th, 2011
Chapters
0:05
Title
1:33
Preparation of Yeast Strains
3:00
Cell Growth and Induction of Mitophagy by Nitrogen Starvation
4:48
Labeling of the Vacuole with CMAC-Arg
6:26
Mounting Live Yeast Cells for Fluorescence Microscopy
7:52
Visualizing Mitophagy by Fluorescence Microscopy
10:02
Conclusion
一个强大的方法来监视传递到细胞器的降解和回收的酵母液泡酸性流明描述。该方法依赖一种基因编码的双发射荧光pH值传感器,和可视化的单个细胞,用荧光显微镜对特定目标细胞器的标签。
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