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DOI :
10.3791/2779-v
•
10:27 min
July 18th, 2011
Chapters
0:05
Title
1:33
Preparation of Yeast Strains
3:00
Cell Growth and Induction of Mitophagy by Nitrogen Starvation
4:48
Labeling of the Vacuole with CMAC-Arg
6:26
Mounting Live Yeast Cells for Fluorescence Microscopy
7:52
Visualizing Mitophagy by Fluorescence Microscopy
10:02
Conclusion
劣化とリサイクルのための酵母の液胞の酸性内腔に細胞小器官の配信を監視するための堅牢なアプローチが説明されています。方法は、遺伝的に符号化されたデュアルエミッション蛍光のpHバイオセンサー、及び蛍光顕微鏡を使用して、個々の細胞の可視化とターゲットの細胞小器官の特定のラベルに依存しています。
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