Name: an in vivo rodent model of contraction-induced injury and non-invasive monitoring of recovery
Uploaded: 2011-05-11T12:00:00
Description: Watch this Scientific Journal Video about An in vivo Rodent Model of Contraction-induced Injury and Non-invasive Monitoring of Recovery at JoVE.com

作者想感谢的核心罗伯特Bloch博士，他的实验室空间和设施，并拉奥博士Gullapalli和大施慷慨捐赠，在马里兰（C - TRIM）为转化成像和磁共振研究中心（MRRC）技术支持。这项工作得到了补助金，RML（K01AR053235和1R01AR059179）国家卫生研究院和肌肉萎缩症协会（＃4278），和赠款，耆那教基金会的JAR。

1， 在体内损伤模型和等距扭矩测量。 <ol><li>这些程序可用于大鼠或小鼠7,17,18。首先，放置在动物仰卧吸入麻醉，使用精确的蒸发器（CAT＃91103，兽医装备公司，普莱森（〜4-5％的异氟醚诱导感应室，然后，通过头锥维护〜2％异氟醚） ，CA）。应用无菌眼科霜（Paralube兽医软膏，PharmaDerm，Floham公园，新泽西州），每只眼睛的角膜，以防止干燥。在实验过程中，动物笼外放置了一?...

<ol>
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“肌肉损伤”已定义，并在许多方面衡量。在6,9病理结果结构破坏是显而易见的，但许多用于评估肌肉损伤，包括在动物实验中使用的的生物标志物的问题，他们通常没有关联武力的损失。肌肉损伤通常被定义内用来检查它的检测的背景下，没有人发现，可以考虑在伤后的收缩变化。由于充分的收缩功能，能坚持，尽管损伤标志物的存在，可能会失去武力的最有效的措施，伤后3 ...

（所有的设备是相同的小鼠和大鼠的踏板除外） <ul><li>芽价值线柜（纽瓦克，06M4718） </li><li>多功能L / O USB - 6221M（美国国家仪器，779808-01） </li><li>步进电机控制器（纽瓦克，16M4189） </li><li>步进电机（纽瓦克，16M4198） </li><li>应变放大器（霍尼韦尔，Sensotec，DV - 05） </li><li>扭矩传感器（霍尼韦尔QWLC - 8M） </li><li>脚踏板和稳定装置（定制，正在申请专利） </li></ul>

an in vivo rodent model of contraction-induced injury and non-invasive monitoring of recovery

肌肉拉伤，医生治疗最常见的投诉之一。一个肌肉损伤通常是单独从患者的病史和体检诊断，但可以不同的临床表现大大取决于在评估肌肉损伤肌肉损伤或肌肉疾病，患者程度的损伤，病人的疼痛的耐受力等临床体征，如压痛，强度，范围的议案，最近，影像学检查，通常是有限的。生物标志物，如血清肌酸磷酸激酶水平，通常与肌肉损伤升高，但他们的水平并不总是力生产的损失相关联。这是来自动物，它提供了一个“直接的措施”损害的组织学研究结果即使如此，但没有考虑到所有的功能丧失。有些人认为，肌肉收缩力的整体健康状况的最全面的衡量。由于肌肉损伤是一个随机事件发生的各种生物力学条件下，它是很难研究。在这里，我们描述了在体内动物模型来测量扭矩，并产生一个可靠的肌肉损伤。我们还描述了我们的模型，从一个在原地隔离肌肉力测量。此外，我们描述了我们的小动物磁共振成像过程。

Watch this Scientific Journal Video about An in vivo Rodent Model of Contraction-induced Injury and Non-invasive Monitoring of Recovery at JoVE.com

An in vivo Rodent Model of Contraction-induced Injury and Non-invasive Monitoring of Recovery

一个<em&gt;在体内</em&gt;损伤的动物模型是描述。该方法采用腓骨神经的皮下位置的优势。测速，授时肌肉活化，圆弧运动，所有预先确定的，并同步使用商业软件。伤后的变化进行监测<em&gt;在体内</em&gt;，使用磁共振成像/光谱。

一个在体内收缩引起的损伤，非侵入性的监测恢复啮齿动物模型

Muscle strains are one of the most  common complaints treated by physicians. A muscle injury is typically diagnosed from the patient history and  physical ...

Research

JoVE Journal

Medicine

An in vivo Rodent Model of Contraction-induced Injury and Non-invasive Monitoring of Recovery

Non-invasive Optical Measurement of Cerebral Metabolism and Hemodynamics in Infants

婴幼儿的脑代谢和血流动力学的非侵入式光学测量

Perinatal brain injury remains a significant cause of infant mortality  and morbidity, but there is not yet an effective bedside tool that can  accurately ...

科研

医学

Non-invasive Assessment of Microvascular and Endothelial Function

非侵入性的评估微血管和内皮功能

The authors have utilized capillaroscopy and forearm blood flow techniques to investigate the role of microvascular dysfunction in pathogenesis of ...

Transplantation into the Anterior Chamber of the Eye for Longitudinal, Non-invasive In vivo Imaging with Single-cell Resolution in Real-time

Transplantation into the Anterior Chamber of the Eye for Longitudinal, Non-invasive In vivo Imaging with Single-cell Resolution in Real-time

纵向，非侵入性的移植到眼睛的前房<em在体内</em&gt;单细胞分辨率的实时成像

Intravital imaging has emerged as an indispensable tool in biological research.  In the process, many imaging techniques have been developed to study ...

An Alkali-burn Injury Model of Corneal Neovascularization in the Mouse

角膜新生血管在小鼠碱烧伤模型

Under normal conditions, the cornea is avascular, and this transparency is essential for maintaining good visual acuity. Neovascularization (NV) of the ...

Murine Model for Non-invasive Imaging to Detect and Monitor Ovarian Cancer Recurrence

小鼠模型的非侵入性成像检测和监测卵巢癌复发

Epithelial ovarian cancer is the most lethal gynecologic malignancy in the United States. Although patients initially respond to the current standard of ...

A Methodological Approach to Non-invasive Assessments of Vascular Function and Morphology

方法论方法血管功能和形态的非侵入性评估

The endothelium is the innermost lining of the vasculature and is involved in the maintenance of vascular homeostasis.  Damage to the endothelium may ...

Use of Ultra-high Field MRI in Small Rodent Models of Polycystic Kidney Disease for In Vivo Phenotyping and Drug Monitoring

Use of Ultra-high Field MRI in Small Rodent Models of Polycystic Kidney Disease for In Vivo Phenotyping and Drug Monitoring

在多囊肾小型啮齿动物模型采用超高场磁共振成像<em&gt;在体内</em&gt;表型和药物监测

Several in vivo pre-clinical studies in Polycystic Kidney Disease (PKD) utilize orthologous rodent models to identify and study the genetic and molecular ...

Depletion of Mouse Cells from Human Tumor Xenografts Significantly Improves Downstream Analysis of Target Cells

从人类肿瘤异种移植的小鼠细胞的消耗显着改善靶细胞下游分析

The use of in vitro cell line models for cancer research has been a useful tool. However, it has been shown that these models fail to reliably mimic ...

Non-invasive Assessments of Subjective and Objective Recovery Characteristics Following an Exhaustive Jump Protocol

根据彻底的跳跃协议进行的主观和客观恢复特征的非侵入性评估

Fast recovery after strenuous exercise is important in sports and is often studied via cryotherapy applications. Cryotherapy has a significant ...

Non-invasive Assessment of Dorsiflexor Muscle Function in Mice

小鼠肌功能的无创评价

Assessment of skeletal muscle contractile function is an important measurement for both clinical and research purposes. Numerous conditions can negatively ...