Name: an in vivo rodent model of contraction-induced injury and non-invasive monitoring of recovery
Uploaded: 2011-05-11T12:00:00
Description: Watch this Scientific Journal Video about An in vivo Rodent Model of Contraction-induced Injury and Non-invasive Monitoring of Recovery at JoVE.com

De auteurs willen graag Dr, Robert Bloch bedanken voor zijn gulle gift van laboratorium-ruimte en faciliteiten en Dr Rao Gullapalli en Da Shi in de Core for Translational Imaging bij Maryland (C-TRIM) en de Magnetic Resonance Research Center (MRRC) voor technische ondersteuning. Dit werk werd ondersteund door subsidies aan RML van de National Institutes of Health (K01AR053235 en 1R01AR059179) en van de Vereniging voor Musculaire Dystrofie (# 4278), en door een subsidie ​​aan JAR van de Jain Foundation.

1. Verwondingen in vivo model en de meting van isometrische koppel. <ol><li> Deze procedures kunnen worden gebruikt voor ratten of muizen 7,17,18. Om te beginnen, plaatst u het dier liggende onder inhalatie-anesthesie (~ 4-5% isofluraan voor de inductie in een inductie kamer, dan ~ 2% isofluraan via een neuskegel voor onderhoud) met behulp van een precisie-verdamper (cat # 91103, Vet Equip, Inc, Pleasanton , CA). Breng een steriel oogheelkundige room (Paralube Vet Zalf, PharmaDerm, Floham Park, NJ)...

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&quot;Muscle schade&quot; is gedefinieerd en gemeten op vele manieren. Structurele schade is zichtbaar in histologische bevindingen 6,9, maar een probleem met veel van de biologische markers gebruikt om de spieren letsel, waaronder die in diervoeding gebruikte studies te beoordelen, is dat ze meestal niet correleren met het verlies van kracht. Spierschade wordt vaak gedefinieerd in het kader van de test gebruikt worden om het te onderzoeken en niemand kan het vinden van rekening voor de veranderingen in de co...

(Alle apparatuur is hetzelfde voor muizen en ratten, behalve voor de voetplaat) <ul><li> BUD Value Line Cabinet (Newark, 06M4718) </li><li> Multifunctioneel l / O USB-6221M (National Instruments, 779808-01) </li><li> Stappenmotor controller (Newark, 16M4189) </li><li> Stepper Motor (Newark, 16M4198) </li><li> Strain Gauge Versterker (Honeywell, Sensotec, DV-05) </li><li> Torque Sensor (Honeywell, QWLC-8M) </li><li> Voetplaat en stabilisatie-apparaat (op maat gemaakte, patent pending) </li></ul>

an in vivo rodent model of contraction-induced injury and non-invasive monitoring of recovery

Muscle stammen zijn een van de meest voorkomende klachten behandeld door de artsen. Een spierblessure is meestal gediagnosticeerd uit de anamnese en lichamelijk onderzoek alleen is echter de klinische presentatie kan sterk verschillen, afhankelijk van de omvang van de schade, de pijn tolerantie, etc. Bij patiënten met een spierblessure of spierziekte, de beoordeling van spierschade is meestal beperkt tot de klinische symptomen, zoals tederheid, kracht, bereik van de beweging, en meer recentelijk, imaging studies. Biologische markers, zoals serum creatine kinase niveaus, zijn meestal verhoogd met een spierblessure, maar hun niveau niet altijd correleren met het verlies van kracht productie. Dit is zelfs het geval bij histologische bevindingen van dieren, die een &quot;directe maatregel&quot; van schade te bieden, maar houden geen rekening met alle verlies van functie. Sommigen hebben beargumenteerd dat de meest uitgebreide maatstaf voor de algemene gezondheid van de spier in contractiele kracht. Omdat spierblessure is een willekeurige gebeurtenis die zich voordoet onder verschillende biomechanische omstandigheden, is het moeilijk om te studeren. We beschrijven hier een in vivo diermodel om het koppel te meten en om een betrouwbare spierblessure te produceren. We beschrijven ook ons model voor het meten van de kracht van een geïsoleerde spier in situ. Verder beschrijven we onze kleine dieren MRI-procedure.

Watch this Scientific Journal Video about An in vivo Rodent Model of Contraction-induced Injury and Non-invasive Monitoring of Recovery at JoVE.com

An in vivo Rodent Model of Contraction-induced Injury and Non-invasive Monitoring of Recovery

Een<em&gt; In vivo</em&gt; Diermodel van schade is beschreven. De methode maakt gebruik van de subcutane positie van de nervus fibularis. Snelheid, timing van de spieren activering, en boog van de beweging zijn alle vooraf bepaalde en gesynchroniseerd met commerciële software. Bericht letsel veranderingen worden bewaakt<em&gt; In vivo</em&gt; Met behulp van MRI / spectroscopie.

Een In vivo Knaagdieren Model van krimp veroorzaakte letsel en niet-invasieve monitoring van de Recovery

Muscle strains are one of the most  common complaints treated by physicians. A muscle injury is typically diagnosed from the patient history and  physical ...

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An in vivo Rodent Model of Contraction-induced Injury and Non-invasive Monitoring of Recovery

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