Name: an in vivo rodent model of contraction-induced injury and non-invasive monitoring of recovery
Uploaded: 2011-05-11T12:00:00
Description: Watch this Scientific Journal Video about An in vivo Rodent Model of Contraction-induced Injury and Non-invasive Monitoring of Recovery at JoVE.com

Gli autori desiderano ringraziare il Dr. Robert Bloch per la sua generosa donazione del laboratorio spaziale e strutture e il dottor Rao Gullapalli e Da Shi nel Nucleo per l&#39;imaging traslazionale a Maryland (C-TRIM) e la Risonanza Magnetica Research Center (MRRC) per il supporto tecnico. Questo lavoro è stato sostenuto dalle concessioni di RML dal National Institutes of Health (K01AR053235 e 1R01AR059179) e dalla Distrofia Muscolare (# 4278), e da una sovvenzione alle JAR dalla Fondazione Jain.

1. Modello in vivo lesioni e la misura di coppia isometrica. <ol><li> Queste procedure possono essere usati per i ratti o topi 7,17,18. Per iniziare, posizionare il supina animale in anestesia inalatoria (~ 4-5% isoflurano per l&#39;induzione in una camera di induzione, allora ~ 2% isoflurano tramite un nosecone per la manutenzione), utilizzando un vaporizzatore di precisione (gatto # 91103, Vet Equip, Inc, Pleasanton , CA). Applicare la crema oftalmica sterile (Unguento Vet Paralube, PharmaDerm, F...

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	<li>Aldridge, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Muscle+pain+after+exercise+is+linked+with+an+inorganic+phosphate+increase+as+shown+by+31P.">Muscle pain after exercise is linked with an inorganic phosphate increase as shown by 31P.</a> NMR. Biosci. Rep. 6, 663-663 (1986).</li><li>Argov, Z., Lofberg, M., Arnold, D. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Insights+into+muscle+diseases+gained+by+phosphorus+magnetic+resonance+spectroscopy.">Insights into muscle diseases gained by phosphorus magnetic resonance spectroscopy.</a> Muscle Nerve. 23, 1316-1316 (2000).</li><li>Brooks, S. V., Zerba, E., Faulkner, J. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Injury+to+muscle+fibres+after+single+stretches+of+passive+and+maximally+stimulated+muscles+in+mice.">Injury to muscle fibres after single stretches of passive and maximally stimulated muscles in mice.</a> J. Physiol. 488, 459-459 (1995).</li><li>Burkholder, T. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Relationship+between+muscle+fiber+types+and+sizes+and+muscle+architectural+properties+in+the+mouse+hindlimb.">Relationship between muscle fiber types and sizes and muscle architectural properties in the mouse hindlimb.</a> J. Morphol. 221, 177-177 (1994).</li><li>Hakim, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Dexamethasone+and+Recovery+of+Contractile+Tension+after+a+Muscle+Injury.">Dexamethasone and Recovery of Contractile Tension after a Muscle Injury.</a> Clin. Orthop. Relat Res. 439, 235-235 (2005).</li><li>Hamer, P. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Evans+Blue+Dye+as+an+in+vivo+marker+of+myofibre+damage:+optimising+parameters+for+detecting+initial+myofibre+membrane+permeability.">Evans Blue Dye as an in vivo marker of myofibre damage: optimising parameters for detecting initial myofibre membrane permeability.</a> J. Anat. 200, 69-69 (2002).</li><li>Hammond, J. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Use+of+Autologous+Platelet-rich+Plasma+to+Treat+Muscle+Strain+Injuries.">Use of Autologous Platelet-rich Plasma to Treat Muscle Strain Injuries.</a> Am. J. Sports Med. , (2009).</li><li>Heemskerk, A. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Determination+of+mouse+skeletal+muscle+architecture+using+three-dimensional+diffusion+tensor+imaging.">Determination of mouse skeletal muscle architecture using three-dimensional diffusion tensor imaging.</a> Magn Reson. Med. 53, 1333-1333 (2005).</li><li>Ho, K. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Skeletal+muscle+fiber+splitting+with+weight-lifting+exercise+in+rats.">Skeletal muscle fiber splitting with weight-lifting exercise in rats.</a> Am. J. Anat. 157, 433-433 (1980).</li><li>Huijing, P. A., Baan, G. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Myofascial+force+transmission+causes+interaction+between+adjacent+muscles+and+connective+tissue:+effects+of+blunt+dissection+and+compartmental+fasciotomy+on+length+force+characteristics+of+rat+extensor+digitorum+longus+muscle.">Myofascial force transmission causes interaction between adjacent muscles and connective tissue: effects of blunt dissection and compartmental fasciotomy on length force characteristics of rat extensor digitorum longus muscle.</a> Arch. Physiol Biochem. 109, 97-97 (2001).</li><li>Ingalls, C. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Dihydropyridine+and+ryanodine+receptor+binding+after+eccentric+contractions+in+mouse+skeletal+muscle.">Dihydropyridine and ryanodine receptor binding after eccentric contractions in mouse skeletal muscle.</a> J. Appl. Physiol. 96, 1619-1619 (2004).</li><li>Lee, D., Marcinek, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Noninvasive+in+vivo+small+animal+MRI+and+MRS:+basic+experimental+procedures.">Noninvasive in vivo small animal MRI and MRS: basic experimental procedures.</a> J. Vis. Exp. , (2009).</li><li>Lovering, R. M., Deyne, P. G. D. e. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Contractile+function,+sarcolemma+integrity,+and+the+loss+of+dystrophin+after+skeletal+muscle+eccentric+contraction-induced+injury.">Contractile function, sarcolemma integrity, and the loss of dystrophin after skeletal muscle eccentric contraction-induced injury.</a> Am. J. Physiol Cell Physiol. 286, C230-C238 (2004).</li><li>Lovering, R. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+contribution+of+contractile+pre-activation+to+loss+of+function+after+a+single+lengthening+contraction.">The contribution of contractile pre-activation to loss of function after a single lengthening contraction.</a> J. Biomech. 38, 1501-1501 (2005).</li><li>Lovering, R. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Recovery+of+function+in+skeletal+muscle+following+2+different+contraction-induced+injuries.">Recovery of function in skeletal muscle following 2 different contraction-induced injuries.</a> Arch. Phys. Med. Rehabil. 88, 617-617 (2007).</li><li>Provencher, S. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Automatic+quantitation+of+localized+in+vivo+1H+spectra+with+LCModel.">Automatic quantitation of localized in vivo 1H spectra with LCModel.</a> NMR Biomed. 14, 260-260 (2001).</li><li>Roche, J. A., Lovering, R. M., Bloch, R. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Impaired+recovery+of+dysferlin-null+skeletal+muscle+after+contraction-induced+injury+in+vivo.">Impaired recovery of dysferlin-null skeletal muscle after contraction-induced injury in vivo.</a> Neuroreport. 19, 1579-1579 (2008).</li><li>Stone, M. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Absence+of+keratin+19+in+mice+causes+skeletal+myopathy+with+mitochondrial+and+sarcolemmal+reorganization.">Absence of keratin 19 in mice causes skeletal myopathy with mitochondrial and sarcolemmal reorganization.</a> J. Cell Sci. 120, 3999-3999 (2007).</li><li>Van Donkelaar, C. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Diffusion+tensor+imaging+in+biomechanical+studies+of+skeletal+muscle+function.">Diffusion tensor imaging in biomechanical studies of skeletal muscle function.</a> J. Anat. 194, 79-79 (1999).</li><li>Vogl, T. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+value+of+in-vivo+31-phosphorus+spectroscopy+in+the+diagnosis+of+generalized+muscular+diseases.+The+clinical+results+and+the+differential+diagnostic+aspects.">The value of in-vivo 31-phosphorus spectroscopy in the diagnosis of generalized muscular diseases. The clinical results and the differential diagnostic aspects.</a> Rofo. 162, 455-455 (1995).</li></ol>

&quot;Danno muscolare&quot; è stato definito e misurato in molti modi. Danni strutturali è evidente in reperti istologici 6,9, ma un problema con molti dei marcatori biologici per valutare lesioni muscolari, compresi quelli utilizzati in studi su animali, è che di solito non sono correlati con la perdita della forza. Danno muscolare è spesso definite nel contesto del test utilizzato per esaminare e trovare nessuno può spiegare le variazioni di contrattilità dopo l&#39;infortunio. Dal piena funzione cont...

(Tutto l&#39;equipaggiamento è lo stesso per topi e ratti ad eccezione della pedana) <ul><li> BUD Value Line Cabinet (Newark, 06M4718) </li><li> Multifunzione l / O USB-6221M (National Instruments, 779808-01) </li><li> Stepper motor controller (Newark, 16M4189) </li><li> Stepper Motor (Newark, 16M4198) </li><li> Amplificatore Strain Gauge (Honeywell, Sensotec, DV-05) </li><li> Sensore di coppia (Honeywell, QWLC-8M) </li><li> Pedana e dispositivo di stabilizzazione (su misura, in attesa di brevetto) </li></ul>

an in vivo rodent model of contraction-induced injury and non-invasive monitoring of recovery

Tensioni muscolari sono uno dei disturbi più comuni trattati dai medici. Una lesione muscolare è di solito diagnosticata dalla storia del paziente e esame fisico da solo, ma la presentazione clinica può variare notevolmente a seconda della entità del danno, tolleranza al dolore del paziente, ecc Nei pazienti con lesioni muscolari o malattia muscolare, la valutazione del danno muscolare è in genere limitata ai segni clinici, come la tenerezza, la forza, la gamma di movimento e, più recentemente, studi di imaging. Marcatori biologici, come i livelli sierici di creatina chinasi, sono in genere elevata con lesioni muscolari, ma i loro livelli non sono sempre correlate con la perdita di produzione di forza. Questo è ancora vero di reperti istologici da animali, che forniscono una &quot;misura diretta&quot; del danno, ma non tengono conto di tutti la perdita della funzione. Alcuni hanno sostenuto che la misura più completa della salute generale del muscolo in forza contrattile. Perché il danno muscolare è un evento casuale che si verifica in una varietà di condizioni biomeccaniche, è difficile da studiare. Qui, descriviamo un modello in vivo su animali per misurare la coppia e per la produzione di un infortunio muscolare affidabile. Abbiamo anche descrivere il nostro modello di misurazione della forza di un muscolo isolato in situ. Inoltre, si descrive la nostra procedura di piccoli animali risonanza magnetica.

Watch this Scientific Journal Video about An in vivo Rodent Model of Contraction-induced Injury and Non-invasive Monitoring of Recovery at JoVE.com

An in vivo Rodent Model of Contraction-induced Injury and Non-invasive Monitoring of Recovery

Un<em&gt; In vivo</emModello animale&gt; di lesioni è descritta. Il metodo sfrutta la posizione sottocutaneo del nervo peroneale. Velocità, tempi di attivazione muscolare, e arco di movimento sono tutti pre-determinato e sincronizzati con il software commerciale. Cambiamenti lesioni post sono monitorati<em&gt; In vivo</em&gt; Utilizzando RM / spettroscopia.

Un In vivo Modello di roditore della contrazione indotta Lesioni e monitoraggio non invasivo di recupero

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An in vivo Rodent Model of Contraction-induced Injury and Non-invasive Monitoring of Recovery

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