Name: an in vivo rodent model of contraction-induced injury and non-invasive monitoring of recovery
Uploaded: 2011-05-11T12:00:00
Description: Watch this Scientific Journal Video about An in vivo Rodent Model of Contraction-induced Injury and Non-invasive Monitoring of Recovery at JoVE.com

著者は、メリーランド州でのトランスレーショナルイメージング（C - TRIM）と磁気共鳴研究センター（MRRC）のためのコアで博士、実験スペースや設備の彼の寛大な寄付のためのロバートBlochと博士ラオGullapalliと大十に感謝します技術サポートのため。この作品は、国立衛生研究所（K01AR053235と1R01AR059179）からと筋ジストロフィー協会（＃4278）からRMLへの補助金によって支え、そしてジャイナ教の財団からJARへの助成金によっていた。

（1） 生体傷害モデルと等尺性トルクの測定インチ <ol><li>これらの手順は、ラットやマウス7,17,18に使用することができます。開始するには、精度の気化器を（猫＃91103、獣医装備、株式会社、プレザントンを使用して（誘導チャンバ内の誘導のためのイソフルラン4〜5％が、その後のメンテナンスのためノーズコーンを介してイソフルランを2％〜に）吸入麻酔下で動物...

<ol>
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&quot;筋肉の損傷は、&quot;多くの方法で定義されており、測定されている。構造上の損傷は、組織学的所見6,9で明らかですが、動物実験で使用されているものを含む筋肉の損傷を、評価するために使用される生物学的マーカーの多くを持つ一つの問題は、彼らは通常の力の喪失と相関しないということです。筋肉の損傷は、しばしばそれを調べるために使用されるアッセイのコンテキ?...

（全ての機器は、踏み板を除いてマウスとラットでも同じです） <ul><li> BUDバリューラインキャビネット（ニューアーク、06M4718） </li><li>多機能L / O USB - 6221M（ナショナルインスツルメンツ、779808〜01） </li><li>ステッピングモータコントローラ（ニューアーク、16M4189） </li><li>ステッピングモータ（ニューアーク、16M4198） </li><li> （ハネウェル、Sensotec、DV - 05）ゲージアンプの歪み</li><li>トルクセンサ（ハネウェル、QWLC - 8M） </li><li>フットプレートと安定化装置（カスタムが作った、特許出願中） </li></ul>

an in vivo rodent model of contraction-induced injury and non-invasive monitoring of recovery

筋肉の菌株は、医師によって治療された最も一般的な苦情の一つです。筋肉の損傷は通常、単独で患者の病歴と身体検査から診断されている、しかし、臨床症状は、筋肉の損傷や筋肉の疾患を有する患者では傷害の程度、患者の痛みの公差等によって大幅に変動しますので、筋肉の損傷の評価は通常、このような動きの優しさ、強さ、範囲、そして最近では、画像検査などの臨床症状、に限定。このような血清クレアチンキナーゼのレベルのような生物学的マーカーが、、一般的に筋肉の傷害で上昇しているが、そのレベルは常に力の生産の損失と相関関係はありません。これは、被害の&quot;直接測定&quot;を提供するが、関数のすべての損失を計上していない動物は、からにも組織学的所見の真です。いくつかの収縮力で筋肉の全体的な健康の最も包括的な措置と主張してきた。筋肉の損傷は生体力学的条件の様々な環境下で発生するランダムイベントなので、勉強することは困難です。ここで、我々 は 、in vivo動物モデルでのトルクを測定することで信頼性の筋肉の損傷を生成するために説明します。我々はまた、 その場で分離された筋肉から力の測定のための我々のモデルについて説明します。さらに、我々は小動物MRIの手順を説明します。

Watch this Scientific Journal Video about An in vivo Rodent Model of Contraction-induced Injury and Non-invasive Monitoring of Recovery at JoVE.com

An in vivo Rodent Model of Contraction-induced Injury and Non-invasive Monitoring of Recovery

アン<em&gt; in vivoで</emけがの&gt;動物モデルが記載されている。方法は、腓骨神経の皮下の位置を利用しています。速度、筋活動のタイミング、および動きの円弧は、すべての商用ソフトウェアを使用して事前に決定し、同期されます。損傷後の変更が監視されます<em&gt; in vivoで</em&gt; MRイメージング/分光法を用いた。

アン in vivoで</em収縮で誘起された損傷と回復の非侵襲的モニタリングの>齧歯類モデル

Muscle strains are one of the most  common complaints treated by physicians. A muscle injury is typically diagnosed from the patient history and  physical ...

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An in vivo Rodent Model of Contraction-induced Injury and Non-invasive Monitoring of Recovery

Non-invasive Optical Measurement of Cerebral Metabolism and Hemodynamics in Infants

乳幼児の脳代謝と血行動態の非侵襲的光計測

Perinatal brain injury remains a significant cause of infant mortality  and morbidity, but there is not yet an effective bedside tool that can  accurately ...

Non-invasive Assessment of Microvascular and Endothelial Function

微小血管と内皮機能の非侵襲的評価

The authors have utilized capillaroscopy and forearm blood flow techniques to investigate the role of microvascular dysfunction in pathogenesis of ...

Transplantation into the Anterior Chamber of the Eye for Longitudinal, Non-invasive In vivo Imaging with Single-cell Resolution in Real-time

Transplantation into the Anterior Chamber of the Eye for Longitudinal, Non-invasive In vivo Imaging with Single-cell Resolution in Real-time

縦用の眼の前房に移植、非侵襲<emインビボ&gt;</emリアルタイムでのシングルセル分解能&gt;イメージング

Intravital imaging has emerged as an indispensable tool in biological research.  In the process, many imaging techniques have been developed to study ...

An Alkali-burn Injury Model of Corneal Neovascularization in the Mouse

マウス角膜血管新生のアルカリ熱傷損傷モデル

Under normal conditions, the cornea is avascular, and this transparency is essential for maintaining good visual acuity. Neovascularization (NV) of the ...

Murine Model for Non-invasive Imaging to Detect and Monitor Ovarian Cancer Recurrence

卵巣がん再発を検出し、モニターするための非侵襲的イメージングのためのマウスモデル

Epithelial ovarian cancer is the most lethal gynecologic malignancy in the United States. Although patients initially respond to the current standard of ...

A Methodological Approach to Non-invasive Assessments of Vascular Function and Morphology

血管機能と形態の非侵襲的なアセスメントに方法論的アプローチ

The endothelium is the innermost lining of the vasculature and is involved in the maintenance of vascular homeostasis.  Damage to the endothelium may ...

Use of Ultra-high Field MRI in Small Rodent Models of Polycystic Kidney Disease for In Vivo Phenotyping and Drug Monitoring

Use of Ultra-high Field MRI in Small Rodent Models of Polycystic Kidney Disease for In Vivo Phenotyping and Drug Monitoring

のための多発性嚢胞腎疾患の小齧歯類モデルにおける超高磁場MRIの使用<em&gt;インビボ</em&gt;表現型と薬物モニタリング

Several in vivo pre-clinical studies in Polycystic Kidney Disease (PKD) utilize orthologous rodent models to identify and study the genetic and molecular ...

Depletion of Mouse Cells from Human Tumor Xenografts Significantly Improves Downstream Analysis of Target Cells

ヒト腫瘍異種移植片からマウス細胞の枯渇は大幅に標的細胞の下流分析を向上させます

The use of in vitro cell line models for cancer research has been a useful tool. However, it has been shown that these models fail to reliably mimic ...

Non-invasive Assessments of Subjective and Objective Recovery Characteristics Following an Exhaustive Jump Protocol

完全なジャンププロトコルに従う主観的および客観的な回復特性の非侵襲的評価

Fast recovery after strenuous exercise is important in sports and is often studied via cryotherapy applications. Cryotherapy has a significant ...

Non-invasive Assessment of Dorsiflexor Muscle Function in Mice

マウスで内がえし筋機能の非侵襲的評価

Assessment of skeletal muscle contractile function is an important measurement for both clinical and research purposes. Numerous conditions can negatively ...

アン in vivoで齧歯類モデル