Name: an in vivo rodent model of contraction-induced injury and non-invasive monitoring of recovery
Uploaded: 2011-05-11T12:00:00
Description: Watch this Scientific Journal Video about An in vivo Rodent Model of Contraction-induced Injury and Non-invasive Monitoring of Recovery at JoVE.com

Yazarlar Maryland Translasyonel Görüntüleme (C-TRIM) ve Manyetik Rezonans Araştırma Merkezi (MRRC) Çekirdek laboratuvar alanı ve tesisleri ve Dr. Rao Gullapalli ve Da Shi yaptığı cömert bağış için Dr Robert Bloch teşekkür etmek istiyorum teknik destek için. Bu çalışma, Jain Vakfı JAR Sağlık (K01AR053235 ve 1R01AR059179) Ulusal Sağlık Enstitüleri ve Müsküler Distrofi Derneği (# 4278), ve hibe RML bağışları ile desteklenmiştir.

1 in vivo yaralanma Model ve izometrik tork ölçümü. <ol><li> Bu prosedürler, sıçan ve fareler 7,17,18 için kullanılabilir . Başlamak için, hassas bir buharlaştırıcı (kedi # 91.103, Veteriner Equip, Inc, Pleasanton inhalasyon anestezi (~% 4-5, bir indüksiyon odasında indüksiyonu için izofluran daha sonra bakım için bir roket ucu konisi yoluyla izofluran% 2 ~) altında hayvan yatar , CA). Kurumasını kornealar korumak için her gözün steril oftalmik krem ​​(Paralube Veteri...

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&quot;Muscle hasar&quot;, birçok şekillerde tanımlanmış ve ölçülmüştür. Yapısal hasar histolojik bulgular 6,9 bellidir, ama birçok hayvan çalışmalarında kullanılan da dahil olmak üzere kas hasarı değerlendirmek için kullanılan biyolojik belirteç bir sorun, genellikle kuvvet kaybı ile korele olmadığını. Kas hasarı genellikle incelemek için kullanılan testin kapsamında tanımlanmış ve hiç bir bulgu yaralanma sonrası kontraktilite değişiklikleri için hesap. Tam kasılma fo...

(Tüm ekipmanlar footplate dışında fareler ve sıçanlar için aynı) <ul><li> BUD Değer Hattı Kabine (Newark, 06M4718) </li><li> Çok Fonksiyonlu l / O USB-6221M (National Instruments, 779.808-01) </li><li> Step motor kontrol (Newark, 16M4189) </li><li> Step Motor (Newark, 16M4198) </li><li> Ölçer Amplifikatör Gerilme (Honeywell, Sensotec, DV-05) </li><li> Tork Sensörü (Honeywell, QWLC-8M) </li><li> Ayak plakası ve istikrar cihazı (özel yapılan patent başvurusu) </li></ul>

an in vivo rodent model of contraction-induced injury and non-invasive monitoring of recovery

Kas suşları doktorlar tarafından tedavi edilen en yaygın şikayetlerden biridir. Ancak bir kas hasarı, genellikle hastanın öyküsü ve fizik muayene tek başına tanısı, klinik prezentasyon, kas hasarı veya kas hastalıkları, kas hasarı değerlendirmesi hastalarda yaralanma, hastanın ağrı toleransı, vb ölçüde büyük ölçüde bağlı olarak değişebilir. hassasiyet, hareket gücü, menzil ve daha yakın zamanda, görüntüleme çalışmaları gibi klinik belirtiler, genellikle sınırlı. Kas hasarı ile serum kreatin kinaz düzeyleri gibi biyolojik belirteçleri, genellikle yükselir, ancak bunların seviyelerini her zaman güç üretim kaybı ile ilişkili değildir. Bu zarar bir &quot;doğrudan ölçmek&quot; sağlayan hayvanlar, histolojik bulgular bile doğrudur, ancak fonksiyon kaybı hesabı yok. Bazı iddia, kas kasılma kuvveti genel sağlık en kapsamlı önlem. Kas hasarı çeşitli biyomekanik koşullar altında meydana gelen rastgele bir olay olduğundan, çalışmak zordur. Burada, in vivo hayvan modelinde bir tork ölçmek için üretmek ve güvenilir bir kas hasarı açıklar. Ayrıca in situ izole kas kuvveti ölçümü için model tarif. Ayrıca, küçük hayvan MRI prosedürü açıklar.

Watch this Scientific Journal Video about An in vivo Rodent Model of Contraction-induced Injury and Non-invasive Monitoring of Recovery at JoVE.com

An in vivo Rodent Model of Contraction-induced Injury and Non-invasive Monitoring of Recovery

Bir<em&gt; In vivo</em&gt; Yaralanma hayvan modeli tarif edilir. Bu yöntem, fibula sinir subkutan pozisyon yararlanır. , Hız, kas aktivasyon zamanlama, hareket ark tüm önceden belirlenmiş ve ticari yazılımı kullanarak senkronize. Mesaj yaralanma değişiklikler izlenir<em&gt; In vivo</em&gt; MR / spektroskopisi kullanarak.

Bir In vivo</emDaralma indüklenen Sakatlık ve Kurtarma Non-invaziv İzleme> Kemirgen Model

Muscle strains are one of the most  common complaints treated by physicians. A muscle injury is typically diagnosed from the patient history and  physical ...

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An in vivo Rodent Model of Contraction-induced Injury and Non-invasive Monitoring of Recovery

Non-invasive Optical Measurement of Cerebral Metabolism and Hemodynamics in Infants

Bebeklerde Serebral Metabolizma ve Hemodinamiğin Noninvaziv Optik Ölçüm

Perinatal brain injury remains a significant cause of infant mortality  and morbidity, but there is not yet an effective bedside tool that can  accurately ...

Non-invasive Assessment of Microvascular and Endothelial Function

Mikrovasküler ve Endotel Fonksiyonu Noninvaziv Değerlendirilmesi

The authors have utilized capillaroscopy and forearm blood flow techniques to investigate the role of microvascular dysfunction in pathogenesis of ...

Transplantation into the Anterior Chamber of the Eye for Longitudinal, Non-invasive In vivo Imaging with Single-cell Resolution in Real-time

Transplantation into the Anterior Chamber of the Eye for Longitudinal, Non-invasive In vivo Imaging with Single-cell Resolution in Real-time

Boyuna, Non-invaziv için Eye Ön Kamara içine Nakli<em&gt; In vivo</emGerçek zamanlı Tek hücreli Çözünürlük&gt; Görüntüleme

Intravital imaging has emerged as an indispensable tool in biological research.  In the process, many imaging techniques have been developed to study ...

An Alkali-burn Injury Model of Corneal Neovascularization in the Mouse

Fare Kornea Neovaskülarizasyonunun bir Alkali-yanık Yaralanma Modeli

Under normal conditions, the cornea is avascular, and this transparency is essential for maintaining good visual acuity. Neovascularization (NV) of the ...

Murine Model for Non-invasive Imaging to Detect and Monitor Ovarian Cancer Recurrence

Non-invaziv Görüntüleme için fare modeli Algılama ve Monitör Yumurtalık Kanseri nüks için

Epithelial ovarian cancer is the most lethal gynecologic malignancy in the United States. Although patients initially respond to the current standard of ...

A Methodological Approach to Non-invasive Assessments of Vascular Function and Morphology

Vasküler Fonksiyon ve Morfoloji Non-invaziv Değerlendirmelerine Bir Metodolojik Yaklaşım

The endothelium is the innermost lining of the vasculature and is involved in the maintenance of vascular homeostasis.  Damage to the endothelium may ...

Use of Ultra-high Field MRI in Small Rodent Models of Polycystic Kidney Disease for In Vivo Phenotyping and Drug Monitoring

Use of Ultra-high Field MRI in Small Rodent Models of Polycystic Kidney Disease for In Vivo Phenotyping and Drug Monitoring

Için Polikistik Böbrek Hastalığında Küçük Kemirgen modelleri Ultra yüksek Saha MRI Kullanımı<em&gt; In Vivo</em&gt; Fenotiplendirme ve İlaç İzleme

Several in vivo pre-clinical studies in Polycystic Kidney Disease (PKD) utilize orthologous rodent models to identify and study the genetic and molecular ...

Depletion of Mouse Cells from Human Tumor Xenografts Significantly Improves Downstream Analysis of Target Cells

İnsan Tümör ksenogratflarının gelen Fare Hücrelerinin tükenmesi Anlamlı Hedef Hücre Alt Analizi geliştirir

The use of in vitro cell line models for cancer research has been a useful tool. However, it has been shown that these models fail to reliably mimic ...

Non-invasive Assessments of Subjective and Objective Recovery Characteristics Following an Exhaustive Jump Protocol

Kapsamlı Bir Atlama Protokolünü takiben Öznel ve Nesnel Kurtarma Özelliklerinin İnvaziv Olmayan Değerlendirmeleri

Fast recovery after strenuous exercise is important in sports and is often studied via cryotherapy applications. Cryotherapy has a significant ...

Non-invasive Assessment of Dorsiflexor Muscle Function in Mice

Non-invaziv Dorsiflexor kas fonksiyonu farelerde değerlendirilmesi

Assessment of skeletal muscle contractile function is an important measurement for both clinical and research purposes. Numerous conditions can negatively ...

Bir In vivo Kemirgen Model