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DOI :
10.3791/2812-v
•
6:09 min
July 31st, 2011
Chapters
0:05
Title
1:05
Trypsin Enzymatic Digestion and Cleanup
3:17
Peptide Immunoaffinity Enrichment
4:45
Analysis by Multiple Reaction Monitoring - Mass Spectrometry
5:24
MRM of a SISCAPA-Enriched Sample
5:44
Conclusion
稳定同位素标准和捕捉反肽抗体(SISCAPA)夫妇亲和富集稳定同位素稀释质谱(MRM - MS)的肽作为代理人为各自的蛋白质肽的定量测量提供。在这里,我们描述了一个部分自动化的格式使用磁性粒子的协议。
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