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DOI :
10.3791/2812-v
•
6:09 min
July 31st, 2011
Chapters
0:05
Title
1:05
Trypsin Enzymatic Digestion and Cleanup
3:17
Peptide Immunoaffinity Enrichment
4:45
Analysis by Multiple Reaction Monitoring - Mass Spectrometry
5:24
MRM of a SISCAPA-Enriched Sample
5:44
Conclusion
安定同位体の標準と抗ペプチド抗体(SISCAPA)によって、それぞれのタンパク質の代用物としてのペプチドの定量的な測定を提供するために、安定同位体希釈質量分析法(MRM - MS)を有するペプチドの結合親和性の濃縮をキャプチャします。ここでは、部分的に自動化された形式で磁性粒子を使用してプロトコルを記述する。
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