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DOI :
10.3791/2821-v
•
7:38 min
June 28th, 2011
Chapters
0:05
Title
1:30
Eye Collection
2:43
Retinal Dissection
3:58
Preparation for Electroporation
4:34
Electroporation
5:27
Culturing of Electroporated Retinas
6:23
Visualization of cis-regulated Element (CRE) Expression in Electroporation Retinal Cells
7:17
Conclusion
このプロトコルは、外植片のエレクトロポレーションを使用してマウスの網膜を生体内でのシス調節エレメント(例えば、エンハンサー/プロモーター)の活性を定量化するための簡単で安価な方法を説明します。 DNA調製、網膜切開、エレクトロポレーション、網膜移植片培養、およびポスト固定分析と定量化が説明されています。
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