Name: evaluation of mammary gland development and function in mouse models
Uploaded: 2011-07-21T12:00:00
Description: Watch this Scientific Journal Video about Evaluation of Mammary Gland Development and Function in Mouse Models at JoVE.com

这些研究是由CIHR CBCRA补助金，以DWL。知识产权是由CIHR - STP，FRSQ和CIHR奖学金资助。 MKGS是由OGS和CIHR - STP奖学金。作者感谢协助他用鼠标育种凯文巴尔。

1。乳腺淋巴清扫术<ol><li>安乐死的鼠标使用CO 2吸入。如果可能，避免颈椎脱位，在颈部和乳腺周围的血液中积累的结果，因为它可能会损害大血管，使剥离更加困难。但是，如果其他条件，在相同的鼠标，如催产素的血药浓度，评估，替代安乐死方法可能是必要的，尽管这必须由您IACUC评估。 </li><li>在铝箔包裹的聚苯乙烯泡沫塑料，在它的后面放置鼠标和蔓延四肢。引脚牢固四肢使用1...

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乳腺发育和功能的紧密协调。突变小鼠中可能隐藏的基因突变，会损害乳腺的发育和功能，突出了需要评估腺架构13,12。本文中描述的乳腺发育的各个阶段，整个安装可以执行。通常情况下，上皮细胞的发展是评估在青春期开始（〜4周龄），青春期（〜6周龄）中，进入青春期后（约10-12周龄）2,10 。在这几个阶段，管分支的数量，可确定的末端芽和管道的长度以量化程度的</sup...

<table border="1"><tbody><tr><th>试剂</th><th>公司</th><th>目录编号</th></tr><tr><td>胭脂红</td><td> Sigma - Aldrich公司</td><td> C1022 </td></tr><tr><td>硫酸铝钾</td><td> Sigma - Aldrich公司</td><td> A6435 </td></tr><tr><td>百里</td><td> Sigma - Aldrich公司</td><td> T0501 </td></tr><tr><td>水杨酸甲酯</td><td> Sigma - Aldrich公司</td><td> M6752 </td></tr><tr><td>催产素</td><td> Sigma - Aldrich公司</td><td> O3251 </td></tr></tbody></table>

evaluation of mammary gland development and function in mouse models

15-20叶组成的人体乳腺分泌乳汁，到乳头管系统开放的一个分支。这些叶是自己的终末导管小叶分泌肺泡和融合的管道1单位组成。在小鼠实验中，观察到类似的架构在怀孕管道和肺泡内的结缔组织基质中穿插。小鼠乳腺上皮细胞是像由两层细胞，管腔细胞基底膜2的范围表示的肌上皮细胞的外层包围的内层组成的导管系统树。出生时，只有残留的导管树目前，主风管和15-20分支机构组成。伸长率和放大分公司开始在青春期的开始，4周龄左右，3,4,5激素的影响下。在10个星期，大部分基质入侵的管道由一个复杂的系统，将发生在每个发情周期，直到怀孕 2分支和回归周期。在怀孕开始，开始第二阶段的发展，与上皮细胞的增殖和分化形成葡萄状的牛奶分泌结构称为肺泡 6,7的。整个分娩和哺乳，牛奶是生产管腔分泌细胞，并储存在肺泡腔内。催产素的释放，仔兔哺乳引起的神经反射刺激，诱导肺泡周围和沿导管的肌上皮细胞的同步收缩，让牛奶通过管道输送到乳头它成为可用的幼崽 8 。乳腺的发育，分化和功能的紧密配合和要求，不仅基质和上皮细胞之间的相互作用，而且之间的肌上皮细胞和管腔内的上皮细胞9,10,11。从而，在这些相互作用中有牵连的许多基因的突变可能会损害或者在妊娠晚期和分泌的激活 ，导致泌乳12,13在青春期或肺泡在怀孕早期，分化形成导管伸长。在这篇文章中，我们描述了如何解剖小鼠乳腺和评估他们的发展，使用整个坐骑。我们还演示了如何评价肌上皮收缩和牛奶弹射使用前体内催产素的功能测定。对乳腺的发育和功能的基因突变的影响，因此可以在原地决定执行这两种技术在突变体和野生型对照组小鼠。

Watch this Scientific Journal Video about Evaluation of Mammary Gland Development and Function in Mouse Models at JoVE.com

Evaluation of Mammary Gland Development and Function in Mouse Models

这种方法介绍如何剖析和评估小鼠乳腺发育和功能。切除乳腺评估使用全挂载的发展程度，而牛奶弹射使用催产素为基础的肌上皮细胞收缩测定评价。

乳腺的发育和功能在小鼠模型的评价

The human mammary gland is composed of 15-20 lobes that secrete milk into a branching duct system opening at the nipple. Those lobes are themselves ...

Research

JoVE Journal

Biology

In vivo Bioluminescent Imaging of Mammary Tumors Using IVIS Spectrum

In vivo Bioluminescent Imaging of Mammary Tumors Using IVIS Spectrum

使用IVIS频谱的乳腺肿瘤在体内生物发光成像

4T1 mouse mammary tumor cells can be implanted sub-cutaneously in nu/nu mice to form palpable tumors in 15 to 20 days. This xenograft tumor model system ...

科研

生物学

Mouse Mammary Epithelial Cells form Mammospheres During Lactogenic Differentiation

小鼠乳腺上皮细胞形成过程中Lactogenic分化的微球体

A phenotypic measure commonly used to determine the degree of lactogenic differentiation in mouse mammary epithelial cell cultures is the formation of ...

In vivo Imaging and Therapeutic Treatments in an Orthotopic Mouse Model of Ovarian Cancer

In vivo Imaging and Therapeutic Treatments in an Orthotopic Mouse Model of Ovarian Cancer

活体成像和治疗卵巢癌的原位小鼠模型

Human cancer and response to therapy is better represented in orthotopic animal models.  This paper describes the development of an orthotopic mouse model ...

Changes in Mammary Gland Morphology and Breast Cancer Risk in Rats

变化在大鼠乳腺形态和患乳腺癌的风险

Studies in rodent models of breast cancer show that exposures to dietary/hormonal factors during the in utero and pubertal periods, when the mammary gland ...

Isolation of Mouse Salivary Gland Stem Cells

鼠标涎腺干细胞的分离

Mature salivary glands of both human and mouse origin comprise a minimum of five cell types, each of which facilitates the production and excretion of ...

Mouse Sperm Cryopreservation and Recovery using the I&middot;Cryo Kit

Mouse Sperm Cryopreservation and Recovery using the IÃƒâ€šÃ‚Â·Cryo Kit

精子冷冻保存和恢复使用I ·冷冻包

Thousands of new genetically modified (GM) strains of mice have been created since the advent of transgenesis and knockout technologies.  Many of these ...

Intraductal Injection for Localized Drug Delivery to the Mouse Mammary Gland

导管内注射局部给药的小鼠乳腺

Herein we describe a protocol to deliver various reagents to the mouse mammary gland via intraductal injections. Localized drug delivery and knock-down of ...

Functional Reconstitution and Channel Activity Measurements of Purified Wildtype and Mutant CFTR Protein

纯化野生型和突变CFTR蛋白的功能重建和通道活性测定

The Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) is a unique channel-forming member of the ATP Binding Cassette (ABC) superfamily of ...

Evaluation of Zebrafish Kidney Function Using a Fluorescent Clearance Assay

斑马鱼肾功能评价采用荧光测定间隙

The zebrafish embryo offers a tractable model to study organogenesis and model human genetic disease. Despite its relative simplicity, the zebrafish ...

Indirect Immunofluorescence on Frozen Sections of Mouse Mammary Gland

对小鼠乳腺冰冻切片间接免疫荧光

Indirect immunofluorescence is used to detect and locate proteins of interest in a tissue. The protocol presented here describes a complete and simple ...