Name: evaluation of mammary gland development and function in mouse models
Uploaded: 2011-07-21T12:00:00
Description: Watch this Scientific Journal Video about Evaluation of Mammary Gland Development and Function in Mouse Models at JoVE.com

इन अध्ययनों CIHR और DWL के लिए CBCRA अनुदान द्वारा वित्त पोषित किया गया. आईपी ​​CIHR एसटीपी, FRSQ और CIHR से फैलोशिप द्वारा वित्त पोषित किया गया था. MKGS OGS और CIHR - एसटीपी छात्रवृत्ति द्वारा वित्त पोषित किया गया था. लेखकों माउस प्रजनन के साथ अपनी सहायता के लिए केविन बर्र धन्यवाद.

1. स्तन ग्रंथि विच्छेदन <ol><li> सीओ 2 साँस लेना माउस का प्रयोग कर euthanize. यदि संभव हो तो ग्रीवा अव्यवस्था से बचें क्योंकि यह गर्दन और स्तन ग्रंथियों के आसपास रक्त के संचय में परिणाम में प्रमुख रक्त वाहिकाओं...

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स्तन ग्रंथि के विकास और समारोह कस करवाया जाता है. उत्परिवर्ती चूहों में जीन म्यूटेशनों कि स्तन ग्रंथि के विकास और समारोह ग्रंथि 13,12 वास्तुकला का मूल्यांकन करने की आवश्यकता पर प्रकाश डाला ख़राब कर ?...

<table border="1"><tbody><tr><th> अभिकर्मक </th><th> कंपनी </th><th> सूचीपत्र संख्या </th></tr><tr><td> कामैन </td><td> सिग्मा Aldrich </td><td> C1022 </td></tr><tr><td> एल्यूमिनियम पोटेशियम सल्फेट </td><td> सिग्मा Aldrich </td><td> A6435 </td></tr><tr><td> थाइमॉल </td><td> सिग्मा Aldrich </td><td> T0501 </td></tr><tr><td> मिथाइल सैलिसिलेट </td><td> सिग्मा Aldrich </td><td> M6752 </td></tr><tr><td> ऑक्सीटोसिन </td><td> सिग्मा Aldrich </td><td> O3251 </td></tr></tbody></table>

evaluation of mammary gland development and function in mouse models

The human mammary gland is composed of 15-20 lobes that secrete milk into a branching duct system opening at the nipple. Those lobes are themselves composed of a number of terminal duct lobular units made of secretory alveoli and converging ducts1. In mice, a similar architecture is observed at pregnancy in which ducts and alveoli are interspersed within the connective tissue stroma. The mouse mammary gland epithelium is a tree like system of ducts composed of two layers of cells, an inner layer of luminal cells surrounded by an outer layer of myoepithelial cells denoted by the confines of a basement membrane2. At birth, only a rudimental ductal tree is present, composed of a primary duct and 15-20 branches. Branch elongation and amplification start at the beginning of puberty, around 4 weeks old, under the influence of hormones3,4,5. At 10 weeks, most of the stroma is invaded by a complex system of ducts that will undergo cycles of branching and regression in each estrous cycle until pregnancy2. At the onset of pregnancy, a second phase of development begins, with the proliferation and differentiation of the epithelium to form grape-shaped milk secretory structures called alveoli6,7. Following parturition and throughout lactation, milk is produced by luminal secretory cells and stored within the lumen of alveoli. Oxytocin release, stimulated by a neural reflex induced by suckling of pups, induces synchronized contractions of the myoepithelial cells around the alveoli and along the ducts, allowing milk to be transported through the ducts to the nipple where it becomes available to the pups 8. Mammary gland development, differentiation and function are tightly orchestrated and require, not only interactions between the stroma and the epithelium, but also between myoepithelial and luminal cells within the epithelium9,10,11. Thereby, mutations in many genes implicated in these interactions may impair either ductal elongation during puberty or alveoli formation during early pregnancy, differentiation during late pregnancy and secretory activation leading to lactation12,13. In this article, we describe how to dissect mouse mammary glands and assess their development using whole mounts. We also demonstrate how to evaluate myoepithelial contractions and milk ejection using an ex-vivo oxytocin-based functional assay. The effect of a gene mutation on mammary gland development and function can thus be determined in situ by performing these two techniques in mutant and wild-type control mice.

Watch this Scientific Journal Video about Evaluation of Mammary Gland Development and Function in Mouse Models at JoVE.com

Evaluation of Mammary Gland Development and Function in Mouse Models

इस विधि का वर्णन कैसे काटना और स्तन ग्रंथि और चूहों से विकास कार्य का आकलन है. Excised स्तन ग्रंथियों पूरे माउंट का उपयोग कर विकास की डिग्री के लिए मूल्यांकन कर रहे हैं जबकि दूध इंजेक्शन एक ऑक्सीटोसिन आधारित myoepithelial सेल संकुचन परख का उपयोग करने के लिए मूल्यांकन किया जाता है.

स्तन ग्रंथि और माउस मॉडल में विकास फंक्शन का मूल्यांकन

The human mammary gland is composed of 15-20 lobes that secrete milk into a branching duct system opening at the nipple. Those lobes are themselves ...

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Biology

In vivo Bioluminescent Imaging of Mammary Tumors Using IVIS Spectrum

In vivo Bioluminescent Imaging of Mammary Tumors Using IVIS Spectrum

स्तन IVIS स्पेक्ट्रम का उपयोग ट्यूमर के vivo bioluminescent इमेजिंग में

4T1 mouse mammary tumor cells can be implanted sub-cutaneously in nu/nu mice to form palpable tumors in 15 to 20 days. This xenograft tumor model system ...

Mouse Mammary Epithelial Cells form Mammospheres During Lactogenic Differentiation

माउस स्तन उपकला कोशिकाओं Lactogenic भेदभाव के दौरान Mammospheres फार्म

A phenotypic measure commonly used to determine the degree of lactogenic differentiation in mouse mammary epithelial cell cultures is the formation of ...

In vivo Imaging and Therapeutic Treatments in an Orthotopic Mouse Model of Ovarian Cancer

In vivo Imaging and Therapeutic Treatments in an Orthotopic Mouse Model of Ovarian Cancer

Vivo इमेजिंग और डिम्बग्रंथि के कैंसर के एक Orthotopic माउस मॉडल में चिकित्सीय उपचार में

Human cancer and response to therapy is better represented in orthotopic animal models.  This paper describes the development of an orthotopic mouse model ...

Changes in Mammary Gland Morphology and Breast Cancer Risk in Rats

स्तन ग्रंथि और चूहे में स्तन कैंसर के खतरे को आकृति विज्ञान में परिवर्तन

Studies in rodent models of breast cancer show that exposures to dietary/hormonal factors during the in utero and pubertal periods, when the mammary gland ...

Isolation of Mouse Salivary Gland Stem Cells

माउस लार ग्रंथि स्टेम सेल के अलगाव

Mature salivary glands of both human and mouse origin comprise a minimum of five cell types, each of which facilitates the production and excretion of ...

Mouse Sperm Cryopreservation and Recovery using the I&middot;Cryo Kit

Mouse Sperm Cryopreservation and Recovery using the IÃƒâ€šÃ‚Â·Cryo Kit

माउस शुक्राणु cryopreservation और रिकवरी मैं · क्रायो किट का उपयोग

Thousands of new genetically modified (GM) strains of mice have been created since the advent of transgenesis and knockout technologies.  Many of these ...

Intraductal Injection for Localized Drug Delivery to the Mouse Mammary Gland

माउस स्तन ग्रंथि के लिए स्थानीय दवा वितरण के लिए intraductal इंजेक्शन

Herein we describe a protocol to deliver various reagents to the mouse mammary gland via intraductal injections. Localized drug delivery and knock-down of ...

Functional Reconstitution and Channel Activity Measurements of Purified Wildtype and Mutant CFTR Protein

शुद्ध wildtype और उत्परिवर्ती CFTR प्रोटीन के कार्यात्मक पुनर्गठन और चैनल गतिविधि माप

The Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) is a unique channel-forming member of the ATP Binding Cassette (ABC) superfamily of ...

Evaluation of Zebrafish Kidney Function Using a Fluorescent Clearance Assay

एक फ्लोरोसेंट क्लीयरेंस परख का उपयोग Zebrafish गुर्दे समारोह का मूल्यांकन

The zebrafish embryo offers a tractable model to study organogenesis and model human genetic disease. Despite its relative simplicity, the zebrafish ...

Indirect Immunofluorescence on Frozen Sections of Mouse Mammary Gland

माउस स्तन ग्रंथि के जमे हुए वर्गों पर अप्रत्यक्ष इम्यूनोफ्लोरेसेंस

Indirect immunofluorescence is used to detect and locate proteins of interest in a tissue. The protocol presented here describes a complete and simple ...