Name: screening assay for oxidative stress in a feline astrocyte cell line, g355-5
Uploaded: 2011-07-13T13:00:00
Description: Watch this Scientific Journal Video about Screening Assay for Oxidative Stress in a Feline Astrocyte Cell Line, G355-5 at JoVE.com

우리는 이러한 연구들의 관대한 지원 ReadiSorb 제품 감사합니다.

1. 세포 고양이 astrocytes의 문화와 H 2 O 2와 치료 <ol><li> 37 DMEM 미디어 플러스 영양소와 75cm이 플라스크에 문화 세포주 G355 - 5 ° C와 5% CO 2 60 % 합류 (~ 5 일)까지. 모든 1~2일 미디어를 교체 또는 건강한 문화를 유지하는 데 필요한. </li><li> 미디어를 제거하고 0.25 %의 트립신 2 ML를 추가합니다. 45 S에 대한 pipetting하여 세포를 리프트 - 실내 온도 (RT)에서 1 분. </li><li> 신속 ...

<ol>
	<li>Bukrinsky, M. I., Nottet, H. S., Schmidtmayerova, H. L., Dubrovsky, H., Flanagan, C. R., Mullins, M. E., Lipton, S. A., Gendelman, H. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Regulation+of+nitric+oxide+synthase+activity+in+human+immunodeficiency+virus+type+1+(HIV-1)-infected+monocytes:+implications+for+HIV-associated+neurological+disease.">Regulation of nitric oxide synthase activity in human immunodeficiency virus type 1 (HIV-1)-infected monocytes: implications for HIV-associated neurological disease.</a> J Exp Med. 181, 735-745 (1995).</li><li>Cadet, J. L., Ali, S., Epstein, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Involvement+of+oxygen-based+radicals+in+methamphetamine-induced+neurotoxicity:+evidence+from+the+use+of+CuZnSOD+transgenic+mice.">Involvement of oxygen-based radicals in methamphetamine-induced neurotoxicity: evidence from the use of CuZnSOD transgenic mice.</a> Ann N Y Acad Sci. 738, 388-3891 (1994).</li><li>Cathcart, R., Schwiers, E., Ames, B. N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Detection+of+picomole+levels+of+hydroperoxides+using+a+fluorescent+dichlorofluorescein+assay.">Detection of picomole levels of hydroperoxides using a fluorescent dichlorofluorescein assay.</a> Anal Biochem. 134, 111-116 (1983).</li><li>Chao, C. C., Hu, S., Peterson, P. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Glia:+the+not+so+innocent+bystanders.">Glia: the not so innocent bystanders.</a> J Neurovirol. 2, 234-239 (1996).</li><li>Cooper, A. J., Kristal, B. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Multiple+roles+of+glutathione+in+the+central+nervous+system.">Multiple roles of glutathione in the central nervous system.</a> Biol Chem. 378, 793-802 (1997).</li><li>Cubells, J. F., Rayport, S., Rajendran, G., Sulzer, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Methamphetamine+neurotoxicity+involves+vacuolation+of+endocytic+organelles+and+dopamine-dependent+intracellular+oxidative+stress.">Methamphetamine neurotoxicity involves vacuolation of endocytic organelles and dopamine-dependent intracellular oxidative stress.</a> J Neurosci. 14, 2260-2271 (1994).</li><li>Dawson, V. L., Dawson, T. M., Uhl, G. R., Snyder, S. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Human+immunodeficiency+virus+type+1+coat+protein+neurotoxicity+mediated+by+nitric+oxide+in+primary+cortical+cultures.">Human immunodeficiency virus type 1 coat protein neurotoxicity mediated by nitric oxide in primary cortical cultures.</a> Proc Natl Acad Sci U S A. 90, 3256-329 (1993).</li><li>Desagher, S., Glowinski, J., Premont, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Astrocytes+protect+neurons+from+hydrogen+peroxide+toxicity.">Astrocytes protect neurons from hydrogen peroxide toxicity.</a> J Neurosci. 16, 2553-2562 (1996).</li><li>Dewhurst, S., Gelbard, H. A., Fine, S. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Neuropathogenesis+of+AIDS.">Neuropathogenesis of AIDS.</a> Mol Med Today. 2, 16-23 (1996).</li><li>Dringen, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Metabolism+and+functions+of+glutathione+in+brain.">Metabolism and functions of glutathione in brain.</a> Prog Neurobiol. 62, 649-671 (2000).</li><li>Dringen, R., Hamprecht, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Glutathione+restoration+as+indicator+for+cellular+metabolism+of+astroglial+cells.">Glutathione restoration as indicator for cellular metabolism of astroglial cells.</a> Dev Neurosci. 20, 401-407 (1998).</li><li>Dringen, R., Pfeiffer, B., Hamprecht, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Synthesis+of+the+Antioxidant+Glutathione+in+Neurons:+Supply+by+Astrocytes+of+CysGly+as+Precursor+for+Neuronal+Glutathione.">Synthesis of the Antioxidant Glutathione in Neurons: Supply by Astrocytes of CysGly as Precursor for Neuronal Glutathione.</a> J Neurosci. 19, 562-569 (1999).</li><li>Everall, I. P. H. u. d. s. o. n., L, R. W. K. e. r. w. i. n. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Decreased+absolute+levels+of+ascorbic+acid+and+unaltered+vasoactive+intestinal+polypeptide+receptor+binding+in+the+frontal+cortex+in+acquired+immunodeficiency+syndrome.">Decreased absolute levels of ascorbic acid and unaltered vasoactive intestinal polypeptide receptor binding in the frontal cortex in acquired immunodeficiency syndrome.</a> Neurosci Lett. 224, 119-1122 (1997).</li><li>Gabriel, C., Camins, A., Sureda, F. X., Aquirre, L., Escubedo, E., Pallas, M., Camarasa, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Determination+of+nitric+oxide+generation+in+mammalian+neurons+using+dichlorofluorescin+diacetate+and+flow+cytometry.">Determination of nitric oxide generation in mammalian neurons using dichlorofluorescin diacetate and flow cytometry.</a> J Pharmacol Toxicol Methods. 38, 93-938 (1997).</li><li>Gendelman, H. E., Genis, P., Jett, M., Zhai, Q. H., Nottet, H. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=An+experimental+model+system+for+HIV-1-induced+brain+injury.">An experimental model system for HIV-1-induced brain injury.</a> Adv Neuroimmunol. 4, 189-1893 (1994).</li><li>Hayman, M., Arbuthnott, G., Harkiss, G., Brace, H., Filippi, P., Philippon, V., Thomson, D., Vigne, R., Wright, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Neurotoxicity+of+peptide+analogues+of+the+transactivating+protein+tat+from+Maedi-Visna+virus+and+human+immunodeficiency+virus.">Neurotoxicity of peptide analogues of the transactivating protein tat from Maedi-Visna virus and human immunodeficiency virus.</a> Neuroscience. 53, 1-6 (1993).</li><li>Hirata, H., Ladenheim, B., Rothman, R. B., Epstein, C., Cadet, J. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Methamphetamine-induced+serotonin+neurotoxicity+is+mediated+by+superoxide+radicals.">Methamphetamine-induced serotonin neurotoxicity is mediated by superoxide radicals.</a> Brain Res. , 677-6345 (1995).</li><li>Koka, P., He, K., Zack, J. A., Kitchen, S., Peacock, W., Fried, I., Tran, T., Yashar, S. S., Merrill, J. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Human+immunodeficiency+virus+1+envelope+proteins+induce+interleukin+1,+tumor+necrosis+factor+alpha,+and+nitric+oxide+in+glial+cultures+derived+from+fetal,+neonatal,+and+adult+human+brain.">Human immunodeficiency virus 1 envelope proteins induce interleukin 1, tumor necrosis factor alpha, and nitric oxide in glial cultures derived from fetal, neonatal, and adult human brain.</a> J Exp Med. 182, 941-951 (1995).</li><li>Kong, L. Y., Wilson, B. C., McMillian, M. K., Bing, G., Hudson, P. M., Hong, J. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+effects+of+the+HIV-1+envelope+protein+gp120+on+the+production+of+nitric+oxide+and+proinflammatory+cytokines+in+mixed+glial+cell+cultures.">The effects of the HIV-1 envelope protein gp120 on the production of nitric oxide and proinflammatory cytokines in mixed glial cell cultures.</a> Cell Immunol. 172, 77-83 (1996).</li><li>Koutsilieri, E., Gotz, M. E., Sopper, S., Sauer, U., Demuth, M., ter Meulen, V., Riederer, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Regulation+of+glutathione+and+cell+toxicity+following+exposure+to+neurotropic+substances+and+human+immunodeficiency+virus-1+in+vitro.">Regulation of glutathione and cell toxicity following exposure to neurotropic substances and human immunodeficiency virus-1 in vitro.</a> J Neurovirol. 3, 342-349 (1997).</li><li>Lipton, S. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Similarity+of+neuronal+cell+injury+and+death+in+AIDS+dementia+and+focal+cerebral+ischemia:+potential+treatment+with+NMDA+open-channel+blockers+and+nitric+oxide-related+species.">Similarity of neuronal cell injury and death in AIDS dementia and focal cerebral ischemia: potential treatment with NMDA open-channel blockers and nitric oxide-related species.</a> Brain Pathol. 6, 507-517 (1996).</li><li>Lipton, S. A., Yeh, M., Dreyer, E. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Update+on+current+models+of+HIV-related+neuronal+injury:+platelet-activating+factor,+arachidonic+acid+and+nitric+oxide.">Update on current models of HIV-related neuronal injury: platelet-activating factor, arachidonic acid and nitric oxide.</a> Adv Neuroimmunol. 4, 181-188 (1994).</li><li>Mills, E. M., Takeda, K., Yu, Z. X., Ferrans, V., Katagiri, Y., Jiang, H., Lavigne, M. C., Leto, T. L., Guroff, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Nerve+growth+factor+treatment+prevents+the+increase+in+superoxide+produced+by+epidermal+growth+factor+in+PC12+cells.">Nerve growth factor treatment prevents the increase in superoxide produced by epidermal growth factor in PC12 cells.</a> J Biol Chem. 273, 22165-22168 (1998).</li><li>Peuchen, S., Duchen, M. R., Clark, J. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Modulation+of+the+glutathione+redox+state+in+adult+astrocytes.">Modulation of the glutathione redox state in adult astrocytes.</a> Biochem Soc Trans. 24, 449S-449S (1996).</li><li>Possel, H., Noack, H., Augustin, W., Keilhoff, G., Wolf, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=2,7-Dihydrodichlorofluorescein+diacetate+as+a+fluorescent+marker+for+peroxynitrite+formation.">2,7-Dihydrodichlorofluorescein diacetate as a fluorescent marker for peroxynitrite formation.</a> FEBS Lett. 416, 175-178 (1997).</li><li>Sagara, J., Makino, N., Bannai, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Glutathione+efflux+from+cultured+astrocytes.">Glutathione efflux from cultured astrocytes.</a> J Neurochem. 66, 1876-1881 (1996).</li><li>Stefano, G. B., Smith, E. M., Paemen, L. R., Hughes, T. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=HIV+gp120+associated+neurological+deficits:+a+potential+role+for+nitric+oxide+and+other+signal+molecules.">HIV gp120 associated neurological deficits: a potential role for nitric oxide and other signal molecules.</a> Advances in Neuroimmunology. 3, 47-57 (1993).</li><li>Sundaresan, M., Yu, Z. X., Ferrans, V. J., Irani, K., Finkel, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Requirement+for+generation+of+H2O2+for+platelet-derived+growth+factor+signal+transduction.">Requirement for generation of H2O2 for platelet-derived growth factor signal transduction.</a> Science. 270, 296-299 (1995).</li><li>Wang, X. F., Cynader, M. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Astrocytes+provide+cysteine+to+neurons+by+releasing+glutathione.">Astrocytes provide cysteine to neurons by releasing glutathione.</a> J Neurochem. 74, 1434-1442 (2000).</li><li>Wilson, J. X. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Antioxidant+defense+of+the+brain:+a+role+for+astrocytes.">Antioxidant defense of the brain: a role for astrocytes.</a> Can J Physiol Pharmacol. 75, 1149-1163 (1997).</li><li>Zenger, E., Collisson, E. W., Barhoumi, R., Burghardt, R. C., Danave, I. R., Tiffany-Castiglioni, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Laser+cytometric+analysis+of+FIV-induced+injury+in+astroglia.">Laser cytometric analysis of FIV-induced injury in astroglia.</a> Glia. 13, 92-100 (1995).</li></ol>

바이러스 자주 제안된 메커니즘의 연결 손상이 산화 스트레스 1, 4, 7, 9, 13, 15, 16, 18-22, 27, 31 유도. 기본적으로, 그것은 제안 이러한 수산화, 초과 산화물의 음이온, 질산 산화물 및 / 또는 신경에​​ 독성이 과산화수소, 같은 바이러스성 노출을 통해 glia (astrocytes와 microglia)가 릴리스 반응 산소 래디 칼. 마찬가지로, 산화 스트레스는 또한 암페타민 유발 neurotoxicity 2, 6, 17의 주요 patho...

<table border="1"><tbody><tr><th> 시약의 이름 </th><th> 회사 </th><th> 카탈로그 번호 </th><th> 댓글 (옵션) </th></tr><tr><td> DMEM </td><td> Cellgro </td><td> 15-013 - CV </td><td></td></tr><tr><td> 멤 비타민 </td><td> Cellgro </td><td> 25-020 - CL </td><td> 1퍼센트 (V / V) </td></tr><tr><td> L - 글루타민 </td><td> Hyclone </td><td> 17 - 605E </td><td> 1퍼센트 (V / V) </td></tr><tr><td> 비 필수 아미노산 </td><td> Hyclone </td><td> 25-025 - CL </td><td> 1퍼센트 (V / V) </td></tr><tr><td> FBS </td><td> Hyclone </td><td> SH30071.03 </td><td> 20% (V / V) </td></tr><tr><td> 필수 아미노산 </td><td> Cellgro </td><td> 25-030 - CL </td><td> 0.2 % (V / V) </td></tr><tr><td> 500ml 진공 여과 시스템 </td><td> VWR </td><td> 87006-076 </td><td></td></tr><tr><td> 15 ML 원뿔 튜브 </td><td> 매 </td><td> 352097 </td><td></td></tr><tr><td> 75cm 두 조직 문화 flasks </td><td> 매 </td><td> 353136 </td><td></td></tr><tr><td> 6 잘 조직 배양 플레이트 </td><td> 매 </td><td> 353224 </td><td></td></tr><tr><td> CM - H 2 DCFDA </td><td> Invitrogen </td><td> C6827 </td><td></td></tr><tr><td> Propidium의 이우 (PI) </td><td> 시그마 </td><td> P4170 - 10mg </td><td></td></tr><tr><td> 트립신 </td><td> Lonza </td><td> 17 - 160F </td><td></td></tr><tr><td> H 2 O 2 </td><td> 시그마 </td><td> H6520 </td><td></td></tr><tr><td> HyQ의 항생제 </td><td> Hyclone </td><td> SV30079.01 </td><td> 0.1 % (V / V) </td></tr><tr><td> G355 - 5 전지 </td><td> ATCC </td><td> CRL - 2033 </td><td> 일반 고양이 뇌 </td></tr></tbody></table>

screening assay for oxidative stress in a feline astrocyte cell line, g355-5

의 연결을 손상을 유도 바이러스의 자주 제안 메커니즘은 산화 스트레스입니다. Astrocytes는 중추 신경계 (CNS)의 산화 스트레스를 조절에 중요​​한 역할을했습니다. Astrocytes는 반응 산소 종 (ROS)에서 뉴런뿐만 아니라 보호하는 뉴런에 생체 항상성 환경을 유지할 수 있도록 도와주십시오. CM - H2DCFDA는 ROS의 존재에 대한 세포 침투성 표시기입니다. CM - H 2 DCFDA이 아닌 형광 화합물로 세포를 입력하고, 휴대폰 esterases은 아세테이트 그룹을 제거한 후 형광되고, 화합물이 산화됩니다. 녹색 fluorescing 것으로 판단될 유동세포계측법에 의해 측정한 세포의 숫자는 산화 상태에있는 세포의 수를 나타냅니다. CM - H 2 DCFDA 다른 ROS 다수에 의해 산화 쉽습니다. 이 분석에 관계없이하는 산소의 산화 세포 환경을 화면으로 사용할 수로 ROS는 CM - H 2 DCFDA을 산화 수있는에 대한 특이성의이 부족은,이 화합물 pathogenesis 조사의 초기 단계에 사용하기 위해 가치있는 리젠트합니다 ROS의 급진 또는 조합은 세포 조건에 책임이 있습니다. 일단 그것은 ROS가 CM - H 2 DCFDA의 산화에 의해 현재 다음 추가 실험이 ROS 또는 로스의 조합이 특정 pathogenesis 과정에 참여하는 결정하기 위해 수행할 수있는 것으로 설립되었습니다. 본 연구의 결과는 과산화수소 CM - H 2 DCFDA의 증가 또한이 분석은 G355 - 5 세포 내에서 산화 환경을 검출을위한 가치있는 테스트입니다 나타내는 생리 컨트롤에 상대적으로 감지되었습니다 형광 것을 입증 고양이 astrocyte 세포 라인.

Watch this Scientific Journal Video about Screening Assay for Oxidative Stress in a Feline Astrocyte Cell Line, G355-5 at JoVE.com

Screening Assay for Oxidative Stress in a Feline Astrocyte Cell Line, G355-5

산화 세포 환경을 감지하는 심사 방법은 CM - H2DCFDA의 산화를 측정하는 것입니다. 일단 비 형광의 형광 화합물로 세포, CM - H2DCFDA 변경 내에 산화. 형광에이 변경 사항은 유동세포계측법으로 측정하고 산화 환경에서 세포의 수를 나타냅니다.

고양이 Astrocyte 세포주에서 산화 스트레스에 대한 심사 분석, G355 - 5

An often-suggested mechanism of virus induced neuronal damage is oxidative stress. Astrocytes have an important role in controlling oxidative stress of ...

Research

JoVE Journal

Neuroscience

Real-Time Impedance-based Cell Analyzer as a Tool to Delineate Molecular Pathways Involved in Neurotoxicity and Neuroprotection in a Neuronal Cell Line

신경 세포주에 신경 독성과 신경에 관여하는 분자 진학의 윤곽을하는 도구로 실시간 임피던스 기반의 세포 분석기

Many brain-related disorders have neuronal cell death involved in their pathophysiology. Improved in vitro models to study neuroprotective or neurotoxic ...

연구

신경과학

The Indirect Neuron-astrocyte Coculture Assay: An In Vitro Set-up for the Detailed Investigation of Neuron-glia Interactions

The Indirect Neuron-astrocyte Coculture Assay: An In Vitro Set-up for the Detailed Investigation of Neuron-glia Interactions

간접 신경-성상 세포 공동 배양 분석 일 :<em&gt; 체외</em&gt; 신경 세포 - glia 상호 작용의 상세한 조사를위한 셋업

Proper neuronal development and function is the prerequisite of the developing and the adult brain. However, the mechanisms underlying the highly ...

Triggering Cell Stress and Death Using Conventional UV Laser Confocal Microscopy

기존의 UV 레이저 공 초점 현미경을 사용하여 트리거 세포 스트레스와 죽음

Using a standard confocal setup, a UV ablation method can be utilized to selectively induce cellular injury and to visualize single-cell responses and ...

Determining Cell-surface Expression and Endocytic Rate of Proteins in Primary Astrocyte Cultures Using Biotinylation

Biotinylation을 사용하여 기본 Astrocyte 문화에서 단백질의 세포 표면 표현 및 Endocytic 속도를 결정

Cell-surface proteins mediate a wide array of functions. In many cases, their activity is regulated by endocytic processes that modulate their levels at ...

Assay Development for High Content Quantification of Sod1 Mutant Protein Aggregate Formation in Living Cells

살아있는 세포에 Sod1 돌연변이 단백질 집계 형성의 높은 콘텐츠 정량화에 대 한 분석 결과 개발

Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease that can be caused by inherited mutations in the gene encoding copper-zinc ...

A Novel In Vitro Live-imaging Assay of Astrocyte-mediated Phagocytosis Using pH Indicator-conjugated Synaptosomes

A Novel In Vitro Live-imaging Assay of Astrocyte-mediated Phagocytosis Using pH Indicator-conjugated Synaptosomes

소설 체 외에 라이브 이미징 분석 결과의 사이토 중재 먹어서 사용 하 여 pH 지시자 활용 Synaptosomes

Astrocytes are the major cell type in the brain and directly contact synapses and blood vessels. Although microglial cells have been considered the major ...

In Vitro Assay for Studying the Aggregation of Tau Protein and Drug Screening

In Vitro Assay for Studying the Aggregation of Tau Protein and Drug Screening

생체 외에서 타우 단백질 및 마약 검사의 집계를 공부에 대 한 분석 결과

Aggregation of tau protein and formation of paired helical filaments is a hallmark of Alzheimer&#39;s disease and other tauopathies. Compared to other ...

In Vivo Calcium Imaging of Lateral-line Hair Cells in Larval Zebrafish

애벌레 Zebrafish의 측면 라인 세포의 생체 내 칼슘 이미징

Sensory hair cells are mechanoreceptors found in the inner ear that are required for hearing and balance. Hair cells are activated in response to sensory ...

Subretinal Transplantation of Human Embryonic Stem Cell-Derived Retinal Tissue in a Feline Large Animal Model

고양이과 거대 동물 모델에서 인간 배아 줄기 세포 유래 망막 조직의 망막하 이식

Retinal degenerative (RD) conditions associated with photoreceptor loss such as age-related macular degeneration (AMD), retinitis pigmentosa (RP) and ...

Cell-Type Specific Protein Purification and Identification from Complex Tissues Using a Mutant Methionine tRNA Synthetase Mouse Line

돌연변이 메티오닌 tRNA 신테타제 마우스 라인을 사용한 복합 조직으로부터의 세포형 특이적 단백질 정제 및 식별

Understanding protein homeostasis in vivo is key to knowing how the cells work in both physiological and disease conditions. The present protocol ...