Name: preparation, purification, and characterization of lanthanide complexes for use as contrast agents for magnetic resonance imaging
Uploaded: 2011-07-21T13:00:00
Description: Watch this Scientific Journal Video about Preparation, Purification, and Characterization of Lanthanide Complexes for Use as Contrast Agents for Magnetic Resonance Imaging at JoVE.com

我们非常感谢来自韦恩州立大学（MJA），从美国的老龄化研究（SMV）基金会的赠款，并以独立职业过渡奖（R00EB007129）来自国家生物医学成像研究所和国家研究院​​生物的途径启动资金卫生（MJA）。

1。 Metalation使用LnCl 3盐<ol><li>溶解在水中的配体产生30-265 mM的解决方案。在这个视频配体2 - （4 - isothiocyanatobenzyl）-二乙三胺五乙酸（P - SCN - BN - DTPA的）浓度在73毫米。 </li><li>通过添加1个M NH 4 OH解决配体的pH值调整到5.5和7.0之间。在这段视频中，1米的NH 4 OH溶液0.2毫升。 </li><li>溶解于水1-2 LnCl 3等值5-1000 mM的浓度与生产的解决方案。在这个视频，EuCl 3</s...

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鉴于越来越多的出版物，包括镧系元素的造影剂4-14，这是重要的准备，净化和表征产品，以确保重现性好，比较的结果，采取照顾。这些复合物通常被认为是具有挑战性的净化和表征有机分子由于其顺磁性质和任何可以使用bioconjugation的功能组别的敏感性相对。我们已经描述了稀土配合物的合成，纯化，并表征的常用方法。然而，选择其中一种方法时，重要的是要考虑的具体制度，正在?...

<table border="1"><tbody><tr><th>试剂和仪器</th><th>公司</th><th>目录编号</th></tr><tr><td> EuCl 3•6H 2 O </td><td> Sigma - Aldrich公司</td><td> 203254 - 5G </td></tr><tr><td> P - SCN - BN - DTPA </td><td> Macrocyclics </td><td> B - 305 </td></tr><tr><td>氢氧化铵</td><td>电解二氧化锰</td><td> AX1303 - 3 </td></tr><tr><td>光谱/ POR生物纤维素酯（CE）的透析膜 - 500 ð截留分子量</td><td> Fisher Scientific则</td><td> 68-671-24 </td></tr><tr><td> Millipore公司IC Millex - LG过滤单元</td><td> Fisher Scientific则</td><td> SLLG C13 NL </td></tr><tr><td>二甲酚橙四钠盐</td><td>阿法埃莎</td><td> 41379 </td></tr><tr><td>乙酸</td><td> Fluka公司</td><td> 49199 </td></tr><tr><td> D 2 O </td><td>剑桥同位素实验室公司</td><td> DLM - 4 - 25 </td></tr><tr><td>净水器</td><td> ELGA </td><td> Purelab超</td></tr><tr><td>高效液相色谱法和质谱</td><td>日本岛津</td><td> LCMS - 2010EV </td></tr><tr><td>弛豫时间分析仪</td><td>布鲁克</td><td> mq60 minispec </td></tr><tr><td>紫外可见分光光度计</td><td> Fisher Scientific则</td><td> 20-624-00092 </td></tr><tr><td>冷冻干燥机</td><td> Fisher Scientific则</td><td> 10-030-133 </td></tr><tr><td>酸度计</td><td> HANNA INSTRUMENTS </td><td>您好221 </td></tr><tr><td>分光光度计</td><td> HORIBA Jobin Yvon公司</td><td> Fluoromax - 4 </td></tr><tr><td>分子量计算器马修梦露6.46版本，下载2009年10月17日，日</td><td> <a href="http://ncrr.pnl.gov/software/">http://ncrr.pnl.gov/software/</a> </td><td>分子量计算器</td></tr></tbody></table>

preparation, purification, and characterization of lanthanide complexes for use as contrast agents for magnetic resonance imaging

常用Polyaminopolycarboxylate基于配体螯合稀土离子，并由此产生的复合物作为磁共振成像（MRI）造影剂。许多市售的配体是特别有用，因为它们包含的功能组别，允许快速，高纯度和高产通过胺反应活性酯和异硫氰酸团体或巯基反应马来酰亚胺的大分子和生物分子共轭。虽然这些配体metalation bioconjugation化学细微的差别，在metalation程序方面的常识，必须考虑选择金属起始原料时。此外，纯化及鉴定的存在，并选择最有效的程序的多个选项部分取决于起始材料的选择。这些细微的差别往往被忽视发布的协议。在这里，我们的目标是展示metalation，纯化和特​​性，可作为MRI（图1）造影剂使用的稀土配合物的常用方法。我们期望本出版物将使生物医学科学家将其纳入自己的常用反应剧目稀土络合反应，由宽松的起始材料和净化方法的选择。

Watch this Scientific Journal Video about Preparation, Purification, and Characterization of Lanthanide Complexes for Use as Contrast Agents for Magnetic Resonance Imaging at JoVE.com

Preparation, Purification, and Characterization of Lanthanide Complexes for Use as Contrast Agents for Magnetic Resonance Imaging

我们展示metalation，净化，稀土配合物的特性。这里所描述的复合物，可以结合大分子，使这些分子利用磁共振成像进行跟踪。

制备，纯化和磁共振成像造影剂的使用稀土配合物的表征

Polyaminopolycarboxylate-based ligands are commonly used to chelate lanthanide ions, and the resulting complexes are 
useful as contrast agents for ...

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