Name: preparation, purification, and characterization of lanthanide complexes for use as contrast agents for magnetic resonance imaging
Uploaded: 2011-07-21T13:00:00
Description: Watch this Scientific Journal Video about Preparation, Purification, and Characterization of Lanthanide Complexes for Use as Contrast Agents for Magnetic Resonance Imaging at JoVE.com

Wij dankbaar erkennen het opstarten van fondsen van Wayne State University (MJA), een subsidie ​​van de Amerikaanse Foundation for Aging Research (SMV) en een Pathway to Independence Career Transition Award (R00EB007129) van het National Institute of Biomedical Imaging and Bioengineering van de National Institutes of Health (MJA).

1. Metalation gebruik LnCl 3 zouten <ol><li> Los het ligand in het water aan een oplossing van 30-265 mM te produceren. Het ligand 2 - (4-isothiocyanatobenzyl)-diethyleentriamine pentaacetic zuur (p-SCN-Bn-DTPA) gebruikt werd in deze video met een concentratie van 73 mM. </li><li> Breng de pH van de oplossing van ligand aan tussen 5,5 en 7,0 door toevoeging van 1 M NH 4 OH. In deze video werd 0,2 ml van de 1 M NH 4 OH-oplossing gebruikt. </li><li> Los 1-2 equivalenten LnCl <su...

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Gezien het toenemende aantal publicaties dat lanthanide-gebaseerde contrastmiddelen 4-14 omvatten, is het belangrijk dat zorg wordt besteed bij de voorbereiding, het zuiveren en karakteriseren van producten aan reproduceerbare en vergelijkbare resultaten te garanderen. Deze complexen worden vaak beschouwd als een uitdaging om te zuiveren en ten opzichte van organische moleculen als gevolg van hun paramagnetisch de natuur en de gevoeligheid van een functionele groepen die kunnen worden gebruikt voor bioconjuga...

<table border="1"><tbody><tr><th> Reagentia en apparatuur </th><th> Vennootschap </th><th> Catalogus nummer </th></tr><tr><td> EuCl 3 • 6H 2 O </td><td> Sigma-Aldrich </td><td> 203254-5G </td></tr><tr><td> p-SCN-Bn-DTPA </td><td> Macrocyclics </td><td> B-305 </td></tr><tr><td> ammonium hydroxide </td><td> EMD </td><td> AX1303-3 </td></tr><tr><td> Spectra / Por Biotech cellulose-ester (CE) dialysemembraan - 500 D MWCO </td><td> Fisher Scientific </td><td> 68-671-24 </td></tr><tr><td> Millipore IC Millex-LG Filter Units </td><td> Fisher Scientific </td><td> SLLG C13 NL </td></tr><tr><td> xylenol oranje tetranatriumzout </td><td> Alfa Aesar </td><td> 41379 </td></tr><tr><td> azijnzuur </td><td> Fluka </td><td> 49199 </td></tr><tr><td> D 2 O </td><td> Cambridge Isotope Laboratories, Inc </td><td> DLM-4-25 </td></tr><tr><td> waterzuiveraar </td><td> ELGA </td><td> Purelab Ultra </td></tr><tr><td> high performance liquid chromatografie en massaspectrometrie </td><td> Shimadzu </td><td> LCMS-2010EV </td></tr><tr><td> relaxatietijd analyzer </td><td> Bruker </td><td> mq60 minispec </td></tr><tr><td> UV-VIS spectrofotometer </td><td> Fisher Scientific </td><td> 20-624-00092 </td></tr><tr><td> vriesdroger </td><td> Fisher Scientific </td><td> 10-030-133 </td></tr><tr><td> pH-meter </td><td> Hanna Instruments </td><td> HI 221 </td></tr><tr><td> Spectrofluorometer </td><td> HORIBA Jobin Yvon </td><td> FluoroMax-4 </td></tr><tr><td> Moleculair gewicht Calculator versie 6.46 van Matthew Monroe, gedownload 17 oktober 2009 </td><td> <a href="http://ncrr.pnl.gov/software/">http://ncrr.pnl.gov/software/</a> </td><td> Moleculair gewicht Calculator </td></tr></tbody></table>

preparation, purification, and characterization of lanthanide complexes for use as contrast agents for magnetic resonance imaging

Polyaminopolycarboxylate-gebaseerde liganden worden vaak gebruikt om lanthanide-ionen-chelaat, en de resulterende complexen zijn bruikbaar als contrastmiddelen voor magnetische resonantie beeldvorming (MRI). Veel commercieel verkrijgbare liganden zijn bijzonder nuttig omdat ze functionele groepen bevatten die het mogelijk maken voor snelle, hoge zuiverheid en hoge opbrengst conjugatie van macromoleculen en biomoleculen via amine-reactieve geactiveerde esters en isothiocyanaatgroepen groepen of thiol-reactieve maleïmiden. Terwijl metalation van deze liganden wordt beschouwd als gemeenschappelijke kennis op het gebied van de bioconjugation chemie, subtiele verschillen in de metalation procedures moet rekening worden gehouden bij de keuze van metaal uitgangsmateriaal. Bovendien meerdere opties voor de zuivering en karakterisering bestaan, en selectie van de meest doeltreffende procedure gedeeltelijk afhankelijk van de keuze van de grondstoffen. Deze subtiele verschillen zijn vaak verwaarloosd in gepubliceerde protocollen. Hier is ons doel om aan te tonen gemeenschappelijke methoden voor het metalation, zuivering en karakterisering van lanthanide complexen die kunnen worden gebruikt als contrastmiddelen voor MRI (figuur 1). We verwachten dat deze publicatie in staat zal stellen biomedische wetenschappers lanthanide complexatie reacties te nemen in hun repertoire van de meest gebruikte reacties door het verlichten van de selectie van de grondstoffen-en zuiveringsmethoden.

Watch this Scientific Journal Video about Preparation, Purification, and Characterization of Lanthanide Complexes for Use as Contrast Agents for Magnetic Resonance Imaging at JoVE.com

Preparation, Purification, and Characterization of Lanthanide Complexes for Use as Contrast Agents for Magnetic Resonance Imaging

We tonen de metalation, zuivering en karakterisering van lanthanide-complexen. De complexen hier beschreven kunnen worden geconjugeerd aan macromoleculen om het volgen van deze moleculen met behulp van magnetische resonantie beeldvorming.

Voorbereiding, Zuivering en karakterisering van lanthanide-complexen voor gebruik als contraststoffen voor Magnetic Resonance Imaging

Polyaminopolycarboxylate-based ligands are commonly used to chelate lanthanide ions, and the resulting complexes are 
useful as contrast agents for ...

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