Name: preparation, purification, and characterization of lanthanide complexes for use as contrast agents for magnetic resonance imaging
Uploaded: 2011-07-21T13:00:00
Description: Watch this Scientific Journal Video about Preparation, Purification, and Characterization of Lanthanide Complexes for Use as Contrast Agents for Magnetic Resonance Imaging at JoVE.com

Wir danken Anschubfinanzierung von der Wayne State University (MJA), einen Zuschuss von der American Foundation for Aging Research (SMV) und ein Pathway to Independence Career Transition Award (R00EB007129) aus dem National Institute of Biomedical Imaging and Bioengineering der National Institutes of Health (MJA).

1. Metallierung mit LnCl 3 Salze <ol><li> Lösen des Liganden in Wasser zu einer Lösung von 30-265 mm. Die Liganden 2 - (4-isothiocyanatobenzyl)-Diethylentriaminpentaessigsäure (p-SCN-Bn-DTPA) wurde in diesem Video bei einer Konzentration von 73 mM verwendet. </li><li> Den pH-Wert der Lösung von Ligand zwischen 5,5 und 7,0 durch Zugabe von 1 M NH 4 OH. In diesem Video wurden 0,2 ml der 1 M NH 4 OH-Lösung verwendet. </li><li> Lösen Sie 1-2 Äquivalente der LnCl 3</sub...

<ol>
	<li>Barge, A., Cravotto, G., Gianolio, E., Fedeli, F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=How+to+determine+free+Gd+and+free+ligand+in+solution+of+Gd+chelates.+A+technical+note.">How to determine free Gd and free ligand in solution of Gd chelates. A technical note.</a> Contrast Med. Mol. Imaging. 1, 184-188 (2006).</li><li>Nagaraja, T. N., Croxen, R. L., Panda, S., Knight, R. A., Keenan, K. A., Brown, S. L., Fenstermacher, J. D., Ewing, J. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Application+of+arsenazo+III+in+the+preparation+and+characterization+of+an+albumin-linked,+gadolinium-based+macromolecular+magnetic+resonance+contrast+agent.">Application of arsenazo III in the preparation and characterization of an albumin-linked, gadolinium-based macromolecular magnetic resonance contrast agent.</a> J. Neurosci. Methods. 157, 238-245 (2006).</li><li>Supkowski, R. M., Horrocks, W. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=On+the+determination+of+the+number+of+water+molecules,+q,+coordinated+to+europium(III)+ions+in+solution+from+luminescence+decay+lifetimes.">On the determination of the number of water molecules, q, coordinated to europium(III) ions in solution from luminescence decay lifetimes.</a> Inorg. Chim. Acta. 340, 44-48 (2002).</li><li>Menjoge, A. R., Kannan, R. M., Tomalia, D. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Dendrimer-based+drug+and+imaging+conjugates:+design+considerations+for+nanomedical+applications.">Dendrimer-based drug and imaging conjugates: design considerations for nanomedical applications.</a> Drug Discovery Today. 15, 171-185 (2010).</li><li>Que, E. L., Chang, C. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Responsive+magnetic+resonance+imaging+contrast+agents+as+chemical+sensors+for+metals+in+biology+and+medicine.">Responsive magnetic resonance imaging contrast agents as chemical sensors for metals in biology and medicine.</a> Chem. Soc. Rev. 39, 51-60 (2010).</li><li>Uppal, R., Caravan, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Targeted+probes+for+cardiovascular+MR+imaging.">Targeted probes for cardiovascular MR imaging.</a> Future Med. Chem. 2, 451-470 (2010).</li><li>Major, J. L., Meade, T. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Bioresponsive,+cell-penetrating,+and+multimeric+MR+contrast+agents.">Bioresponsive, cell-penetrating, and multimeric MR contrast agents.</a> Acc. Chem. Res. 42, 893-903 (2009).</li><li>Datta, A., Raymond, K. N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Gd-hydroxypyridinone+(HOPO)-based+high-relaxivity+magnetic+resonance+imaging+(MRI)+contrast+agents.">Gd-hydroxypyridinone (HOPO)-based high-relaxivity magnetic resonance imaging (MRI) contrast agents.</a> Acc. Chem. Res. 42, 938-947 (2009).</li><li>Le&#243;n-Rodr&#237;guez, L. M. D., Lubag, A. J. M., Malloy, C. R., Martinez, G. V., Gillies, R. J., Sherry, A. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Responsive+MRI+agents+for+sensing+metabolism+in+vivo.">Responsive MRI agents for sensing metabolism in vivo.</a> Acc. Chem. Res. 42, 948-957 (2009).</li><li>Castelli, D. D., Gianolio, E., Crich, S. G., Terreno, E., Aime, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Metal+containing+nanosized+systems+for+MR-molecular+imaging+applications.">Metal containing nanosized systems for MR-molecular imaging applications.</a> Coord. Chem. Rev. 252, 2424-2443 (2008).</li><li>Caravan, P., Ellison, J. J., McMurry, T. J., Lauffer, R. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Gadolinium(III)+chelates+as+MRI+contrast+agents:+structure,+dynamics,+and+applications.">Gadolinium(III) chelates as MRI contrast agents: structure, dynamics, and applications.</a> Chem. Rev. 99, 2293-2352 (1999).</li><li>Lauffer, R. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Paramagnetic+metal+complexes+as+water+proton+relaxation+agents+for+NMR+imaging:+theory+and+design.">Paramagnetic metal complexes as water proton relaxation agents for NMR imaging: theory and design.</a> Chem. Rev. 87, 901-927 (1987).</li><li>Yoo, B., Pagel, <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=An+overview+of+responsive+MRI+contrast+agents+for+molecular+imaging.">An overview of responsive MRI contrast agents for molecular imaging.</a> Front. Biosci. 13, 1733-1752 (2008).</li><li>Pandya, S., Yu, J., Parker, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Engineering+emissive+europium+and+terbium+complexes+for+molecular+imaging+and+sensing.">Engineering emissive europium and terbium complexes for molecular imaging and sensing.</a> Dalton Trans. 23, 2757-2766 (2006).</li><li>Nwe, K., Xu, H., Regino, C. A. S., Bernardo, M., Ileva, L., Riffle, L., Wong, K. J., Brechbiel, M. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+new+approach+in+the+preparation+of+dendrimer-based+bifunctional+diethylenetriaminepentaacetic+acid+MR+contrast+agent+derivatives.">A new approach in the preparation of dendrimer-based bifunctional diethylenetriaminepentaacetic acid MR contrast agent derivatives.</a> Bioconjugate Chem. 20, 1412-1418 (2009).</li><li>Nwe, K., Bernardo, M., Regino, C. A. S., Williams, M., Brechbiel, M. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Comparison+of+MRI+properties+between+derivatized+DTPA+and+DOTA+gadolinium-dendrimer+conjugates.">Comparison of MRI properties between derivatized DTPA and DOTA gadolinium-dendrimer conjugates.</a> Bioorg. Med. Chem. 18, 5925-5931 (2010).</li><li>Caravan, P., Das, B., Deng, Q., Dumas, S., Jacques, V., Koerner, S. K., Kolodziej, A., Looby, R. J., Sun, W. -. C., Zhang, Z. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+lysine+walk+to+high+relaxivity+collagen-targeted+MRI+contrast+agents.">A lysine walk to high relaxivity collagen-targeted MRI contrast agents.</a> Chem. Commun. , 430-432 (2009).</li><li>Le&#243;n-Rodr&#237;guez, L. M. D., Kovacs, Z. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+synthesis+and+chelation+chemistry+of+DOTA-peptide+conjugates.">The synthesis and chelation chemistry of DOTA-peptide conjugates.</a> Bioconjugate Chem. 19, 391-402 (2008).</li><li>Boswell, C. A., Eck, P. K., Regino, C. A. S., Bernardo, M., Wong, K. J., Milenic, D. E., Choyke, P. L., Brechbiel, M. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Synthesis,+characterization,+and+biological+evaluation+of+integrin+&#945;V&#946;3-targeted+PAMAM+dendrimers.">Synthesis, characterization, and biological evaluation of integrin &#945;V&#946;3-targeted PAMAM dendrimers.</a> Mol. Pharm. 5, 527-539 (2008).</li></ol>

Angesichts der zunehmenden Zahl von Publikationen, die Lanthanoid-basierte Kontrastmittel 4-14 zählen, ist es wichtig, dass Pflege in der Vorbereitung, Reinigung und Charakterisierung von Produkten, um reproduzierbare und vergleichbare Ergebnisse zu gewährleisten, wird übernommen. Diese Komplexe werden oft als schwierig zu reinigen und zu charakterisieren gegenüber organischen Molekülen aufgrund ihrer paramagnetischen Natur und die Empfindlichkeit eines funktionellen Gruppen, die für Biokonjugation verw...

<table border="1"><tbody><tr><th> Reagenzien und Geräte </th><th> Firma </th><th> Katalog-Nummer </th></tr><tr><td> EuCl 3 • 6 H 2 O </td><td> Sigma-Aldrich </td><td> 203254-5G </td></tr><tr><td> p-SCN-Bn-DTPA </td><td> Makrocyclen </td><td> B-305 </td></tr><tr><td> Ammoniumhydroxid </td><td> EMD </td><td> AX1303-3 </td></tr><tr><td> Spectra / Por Biotech Cellulose-Ester (CE) Dialysemembran - 500 D MWCO </td><td> Fisher Scientific </td><td> 68-671-24 </td></tr><tr><td> Millipore IC Millex-LG Filter Units </td><td> Fisher Scientific </td><td> SLLG C13 NL </td></tr><tr><td> Xylenolorange Tetranatriumsalz </td><td> Alfa Aesar </td><td> 41379 </td></tr><tr><td> Essigsäure </td><td> Fluka </td><td> 49199 </td></tr><tr><td> D 2 O </td><td> Cambridge Isotope Laboratories, Inc. </td><td> DLM-4-25 </td></tr><tr><td> Wasserreiniger </td><td> ELGA </td><td> Purelab Ultra- </td></tr><tr><td> Hochleistungs-Flüssigkeits-Chromatographie und Massenspektrometrie </td><td> Shimadzu </td><td> LCMS-2010EV </td></tr><tr><td> Relaxationszeit Analysator </td><td> Bruker </td><td> mq60 minispec </td></tr><tr><td> UV-VIS Spektralphotometer </td><td> Fisher Scientific </td><td> 20-624-00092 </td></tr><tr><td> Gefriertrockner </td><td> Fisher Scientific </td><td> 10-030-133 </td></tr><tr><td> pH-Meter </td><td> Hanna Instruments </td><td> HALLO 221 </td></tr><tr><td> Spektrofluorometer </td><td> HORIBA Jobin Yvon </td><td> Fluoromax-4 </td></tr><tr><td> Molecular Weight Calculator Version 6,46 von Matthew Monroe, 17. Oktober 2009 heruntergeladen </td><td> <a href="http://ncrr.pnl.gov/software/">http://ncrr.pnl.gov/software/</a> </td><td> Molecular Weight Calculator </td></tr></tbody></table>

preparation, purification, and characterization of lanthanide complexes for use as contrast agents for magnetic resonance imaging

Polyaminopolycarboxylate-Liganden werden häufig verwendet, um Lanthanidionen Chelat, und die resultierenden Komplexe eignen sich als Kontrastmittel für die Kernspintomographie (MRI). Viele im Handel erhältliche Liganden sind besonders nützlich, da sie funktionelle Gruppen, die für schnelle, hochreine ermöglichen, und mit hoher Ausbeute Konjugation an Makromolekülen und Biomolekülen über aminreaktiven aktivierten Estern und Isothiocyanat-Gruppen oder Thiol-reaktive Maleinimide enthalten. Während Metallierung dieser Liganden wird als allgemein bekannt, im Bereich der Biokonjugation Chemie, subtile Unterschiede in Metallierung Verfahren müssen berücksichtigt werden bei der Auswahl Metall Ausgangsstoffe. Darüber hinaus mehrere Optionen für die Reinigung und Charakterisierung existieren, und die Auswahl der effektivsten Verfahren teilweise abhängig von der Auswahl der Ausgangsstoffe. Diese feinen Unterschiede sind oft in veröffentlichten Protokollen vernachlässigt. Hier ist es unser Ziel, gemeinsame Methoden für die Metallierung, Reinigung und Charakterisierung von Lanthanoid-Komplexe, die als Kontrastmittel für die MRT (Abb. 1) verwendet werden können demonstrieren. Wir erwarten, dass diese Publikation ermöglicht biomedizinische Wissenschaftler Lanthanoid Komplexierung Reaktionen in ihr Repertoire von häufig verwendeten Reaktionen integrieren durch Lockerung der Auswahl der Ausgangsstoffe und Reinigungsverfahren.

Watch this Scientific Journal Video about Preparation, Purification, and Characterization of Lanthanide Complexes for Use as Contrast Agents for Magnetic Resonance Imaging at JoVE.com

Preparation, Purification, and Characterization of Lanthanide Complexes for Use as Contrast Agents for Magnetic Resonance Imaging

Wir zeigen die Metallierung, Reinigung und Charakterisierung von Lanthanoid-Komplexen. Die hier beschriebenen Komplexe können konjugiert werden, um Makromoleküle, damit diese Rückverfolgung Moleküle mittels Magnetresonanztomographie.

Herstellung, Reinigung und Charakterisierung von Lanthanoidkomplexe für die Verwendung als Kontrastmittel für die Magnetresonanz-Tomographie

Polyaminopolycarboxylate-based ligands are commonly used to chelate lanthanide ions, and the resulting complexes are 
useful as contrast agents for ...

Research

JoVE Journal

Biology

Labeling hESCs and hMSCs with Iron Oxide Nanoparticles for Non-Invasive in vivo Tracking with MR Imaging

Labeling hESCs und hMSCs mit Eisenoxid-Nanopartikel für Non-Invasive in vivo-Tracking mit MR-Bildgebung

In recent years, stem cell research has led to a better understanding of developmental biology, various diseases and its potential impact on regenerative ...

Forschung

Biologie

Labeling Stem Cells with Fluorescent Dyes for non-invasive Detection with Optical Imaging

Labeling Stammzellen mit fluoreszierenden Farbstoffen zur nicht-invasiven Detektion mit Optical Imaging

Optical imaging (OI) is an easy, fast and inexpensive tool for in vivo monitoring of new stem cell based therapies. The technique is based on ex vivo ...

In vitro Labeling of Human Embryonic Stem Cells for Magnetic Resonance Imaging

In-vitro-Markierung von humanen embryonalen Stammzellen für Magnetic Resonance Imaging

Human embryonic stem cells (hESC) have demonstrated the ability to restore the injured myocardium. Magnetic resonance imaging (MRI) has emerged as one of ...

Phase Contrast and Differential Interference Contrast (DIC) Microscopy

Phase Contrast and Differential Interference Contrast DIC Microscopy

Phasenkontrast und Differential Interferenz Kontrast (DIC) Mikroskopie

Phase-contrast microscopy is often used to produce contrast for transparent, non light-absorbing, biological specimens. The technique was discovered by ...

Isolation and Purification of Drosophila Peripheral Neurons by Magnetic Bead Sorting

Isolation and Purification of Drosophila Peripheral Neurons by Magnetic Bead Sorting

Isolierung und Reinigung von Drosophila Peripheren Neuronen durch Magnetic Bead Sorting

The Drosophila peripheral nervous system (PNS) is a powerful model for investigating the complex processes of neuronal development and dendrite ...

Microinjection of Medaka Embryos for use as a Model Genetic Organism

Mikroinjektion von Medaka Embryonen für die Verwendung als Modell Genetic Organism

In this video, we demonstrate the technique of microinjection into one-cell stage medaka embryos. Medaka is a small egg-laying freshwater fish that allows ...

Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification

Automatisierte Hydrophobe Interaktions-Chromatographie Spaltenauswahl für den Einsatz in Protein Purification

In contrast to other chromatographic methods for purifying proteins (e.g. gel filtration, affinity, and ion exchange), hydrophobic interaction ...

Quantifying Mixing using Magnetic Resonance Imaging

Quantifizierung Mixing mit Magnetic Resonance Imaging

Mixing is a unit operation that combines two or more components into a homogeneous mixture.  This work involves mixing two viscous liquid streams using an ...

Generation and Purification of Human INO80 Chromatin Remodeling Complexes and Subcomplexes

Generation und Reinigung von Menschen INO80 Chromatin Remodeling Komplexe und Subkomplexe

INO80 chromatin remodeling complexes regulate nucleosome dynamics and DNA accessibility by catalyzing ATP-dependent nucleosome remodeling. Human INO80 ...

FRET Imaging in Three-dimensional Hydrogels

FRET-Imaging im dreidimensionalen Hydrogelen

Imaging of F&#246;rster resonance energy transfer (FRET)&#160;is a powerful tool for examining cell biology in real-time. Studies utilizing FRET commonly ...