Name: preparation, purification, and characterization of lanthanide complexes for use as contrast agents for magnetic resonance imaging
Uploaded: 2011-07-21T13:00:00
Description: Watch this Scientific Journal Video about Preparation, Purification, and Characterization of Lanthanide Complexes for Use as Contrast Agents for Magnetic Resonance Imaging at JoVE.com

Noi riconosciamo con gratitudine fondi avvio da Wayne State University (MJA), una borsa di studio dalla Fondazione Americana per Aging Research (SMV), e un percorso di transizione Career Award Indipendenza (R00EB007129) dal National Institute of Biomedical Imaging e Bioingegneria del National Institutes della Sanità (MJA).

1. Metalation utilizzando LnCl 3 sali <ol><li> Sciogliere il ligando in acqua per produrre una soluzione di 30-265 mM. Il ligando 2 - (4-isothiocyanatobenzyl)-dietilentriammina acido pentaacetic (p-SCN-Bn-DTPA) è stato utilizzato in questo video in una concentrazione di 73 mm. </li><li> Regolare il pH della soluzione di ligando compreso tra 5,5 e 7,0 con l&#39;aggiunta di 1 M NH 4 OH. In questo video, 0,2 ml di M 1 soluzione di NH 4 OH è stato utilizzato. </li><li> Scioglier...

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Dato il numero crescente di pubblicazioni che includono lantanidi a base di agenti di contrasto 4-14, è importante che la cura nella preparazione, purificazione e caratterizzazione dei prodotti per garantire risultati riproducibili e comparabili. Questi complessi sono spesso considerati difficili da purificare e caratterizzare rispetto alle molecole organiche a causa della loro natura paramagnetica e la sensibilità di gruppi funzionali che potrebbero essere utilizzati per bioconjugation. Abbiamo descritto i...

<table border="1"><tbody><tr><th> Reagenti e attrezzature </th><th> Azienda </th><th> Numero di catalogo </th></tr><tr><td> EuCl 3 • 6H 2 O </td><td> Sigma-Aldrich </td><td> 203254-5G </td></tr><tr><td> p-SCN-Bn-DTPA </td><td> Macrocyclics </td><td> B-305 </td></tr><tr><td> idrossido di ammonio </td><td> EMD </td><td> AX1303-3 </td></tr><tr><td> Spectra / Por Biotech Cellulosa Ester (CE) membrana di dialisi - 500 D MWCO </td><td> Fisher Scientific </td><td> 68-671-24 </td></tr><tr><td> Millipore IC Millex-LG Filtro Unità </td><td> Fisher Scientific </td><td> SLLG C13 NL </td></tr><tr><td> xilenolo arancione sale tetrasodico </td><td> Alfa Aesar </td><td> 41379 </td></tr><tr><td> acido acetico </td><td> Fluka </td><td> 49199 </td></tr><tr><td> D 2 O </td><td> Cambridge Isotope Laboratories, Inc. </td><td> DLM-4-25 </td></tr><tr><td> purificatore d&#39;acqua </td><td> ELGA </td><td> Purelab Ultra </td></tr><tr><td> cromatografia liquida ad alta prestazione e la spettrometria di massa </td><td> Shimadzu </td><td> LCMS-2010EV </td></tr><tr><td> tempo di rilassamento analizzatore </td><td> Bruker </td><td> mq60 minispec </td></tr><tr><td> Spettrofotometro UV-vis </td><td> Fisher Scientific </td><td> 20-624-00092 </td></tr><tr><td> congelare asciugatrice </td><td> Fisher Scientific </td><td> 10-030-133 </td></tr><tr><td> pH-metro </td><td> Hanna Instruments </td><td> HI 221 </td></tr><tr><td> spettrofluorimetro </td><td> HORIBA Jobin Yvon </td><td> Fluoromax-4 </td></tr><tr><td> Molecular Weight Calculator versione 6,46 da Matthew Monroe, scaricato 17 Ottobre 2009 </td><td> <a href="http://ncrr.pnl.gov/software/">http://ncrr.pnl.gov/software/</a> </td><td> Peso Molecolare Calculator </td></tr></tbody></table>

preparation, purification, and characterization of lanthanide complexes for use as contrast agents for magnetic resonance imaging

Polyaminopolycarboxylate a base di leganti sono comunemente utilizzati per chelare ioni lantanidi, ed i complessi risultanti sono utili come agenti di contrasto per risonanza magnetica (MRI). Molti ligandi disponibili in commercio sono particolarmente utili perché contengono gruppi funzionali che consentono veloci, elevata purezza e ad alto rendimento coniugazione a macromolecole e biomolecole attraverso esteri attivati ​​ammina-reattiva e gruppi isotiocianato o tiolo-reattiva maleimides. Mentre metalation di questi ligandi è considerato conoscenza comune nel campo della chimica bioconjugation, sottili differenze nelle procedure metalation devono essere presi in considerazione nella scelta dei materiali metallici di partenza. Inoltre, più opzioni per la purificazione e la caratterizzazione esistono, e la scelta della procedura più efficace dipende in parte dalla selezione delle materie prime. Queste sottili differenze sono spesso trascurati nei protocolli pubblicati. Qui, il nostro obiettivo è quello di dimostrare metodi comuni per metalation, purificazione e caratterizzazione di complessi di lantanidi, che possono essere usati come agenti di contrasto per risonanza magnetica (Figura 1). Ci aspettiamo che questa pubblicazione permetteranno agli scienziati biomedici di incorporare le reazioni di complessazione lantanidi nel loro repertorio di reazioni comunemente usate dai facilitando la selezione delle materie prime e metodi di purificazione.

Watch this Scientific Journal Video about Preparation, Purification, and Characterization of Lanthanide Complexes for Use as Contrast Agents for Magnetic Resonance Imaging at JoVE.com

Preparation, Purification, and Characterization of Lanthanide Complexes for Use as Contrast Agents for Magnetic Resonance Imaging

Dimostriamo la metalation, purificazione e caratterizzazione di complessi di lantanidi. I complessi qui descritto può essere coniugato con macromolecole di attivare il rilevamento di queste molecole usando la risonanza magnetica.

Preparazione, purificazione e caratterizzazione di complessi lantanidi per l&#39;uso di agenti di contrasto per Risonanza Magnetica

Polyaminopolycarboxylate-based ligands are commonly used to chelate lanthanide ions, and the resulting complexes are 
useful as contrast agents for ...

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