Name: cecal ligation puncture procedure
Uploaded: 2011-05-07T14:00:00
Description: Watch this Scientific Journal Video about Cecal Ligation Puncture Procedure at JoVE.com

इस काम के स्वास्थ्य के पेंसिल्वेनिया विभाग से अनुदान द्वारा समर्थित किया गया था. डॉ. Miguel Garcia Toscano मंदिर विश्वविद्यालय में एक postdoctoral साथी, इस अध्ययन के दौरान Alfonso मार्टिन Escudero फाउंडेशन द्वारा वित्त पोषित किया गया था. हम वीडियो के बनाने के लिए Iliya Yordanov और केविन Kotredes शुक्रिया अदा करना चाहता हूँ.

1. Cecal और मानव पूति के लिए एक माउस मॉडल के रूप में Ligation पंचर / 6 चूहों (7-9 पुराने सप्ताह) इस प्रक्रिया C57BL के लिए उपयोग किया जाता है. <ol><li> Intraperitoneally 01:01 (75mg/kg) ketamine और xylazine (15mg/kg) के एक समाधान इंजेक्शन द्वारा माउस anesthetize. ...

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यहाँ हम विस्तार से बताते हैं कि कैसे चूहों में CLP मॉडल का प्रदर्शन करने के लिए और गंभीरता के ग्रेड मिलाना. पूति के अन्य पशु मॉडल की तुलना में, CLP विभिन्न आयु और लिंग के किसी भी माउस तनाव में प्रदर...

<table border="1"><tbody><tr><th> अभिकर्मक के नाम </th><th> कंपनी </th><th> सूचीपत्र संख्या </th><th> टिप्पणियाँ </th></tr><tr><td> Ketamine </td><td> Ketaset </td><td> 0856-2013-01 </td><td></td></tr><tr><td> Xylazine </td><td> AnaSed </td><td> 139-236 # नाडा </td><td></td></tr><tr><td> इंसुलिन सिरिंज 29G </td><td> Exel </td><td> 26028 </td><td></td></tr><tr><td> सिल्क सीवन, 6-0 PROLENE </td><td> Ethicon </td><td> 8680G </td><td></td></tr><tr><td> 19G और 25g सुई </td><td> BD </td><td> 305186 </td><td></td></tr><tr><td> रेज़र </td><td> जनरल आपूर्ति </td><td> जनरल आपूर्ति </td><td></td></tr><tr><td> इन्फ्रारेड ताप दीपक </td><td> जनरल आपूर्ति </td><td> जनरल आपूर्ति </td><td></td></tr><tr><td> मिशेल क्लिप 7mm घाव </td><td> Roboz सर्जिकल निर्देप्राप्तगुम </td><td> RS-9270 </td><td></td></tr><tr><td> कान लूप मास्क </td><td> फिशर साइंटिफिक </td><td> 19-130-4181 </td><td></td></tr><tr><td> विच्छेदन कैंची </td><td> Roboz सर्जिकल निर्देप्राप्तगुम </td><td> RS-6702 </td><td></td></tr><tr><td> Betadine समाधान </td><td> VWR </td><td> 63410-992 </td><td></td></tr><tr><td> सर्जिकल संदंश </td><td> Roboz सर्जिकल निर्देप्राप्तगुम </td><td> RS-5135 </td><td></td></tr><tr><td> 70% Isopropyl शराब पैड </td><td> फिशर साइंटिफिक </td><td> 22-031-350 </td><td></td></tr><tr><td> Buprenorphine </td><td> बेडफोर्ड लैब्स </td><td> 55390-100-10 </td><td></td></tr><tr><td> Tramadol </td><td> सिग्मा Aldrich </td><td> 42965 </td><td></td></tr></tbody></table>

cecal ligation puncture procedure

Human sepsis is characterized by a set of systemic reactions in response to intensive and massive infection that failed to be locally contained by the host. Currently, sepsis ranks among the top ten causes of mortality in the USA intensive care units 1. During sepsis there are two established haemodynamic phases that may overlap. The initial phase (hyperdynamic) is defined as a massive production of proinflammatory cytokines and reactive oxygen species by macrophages and neutrophils that affects vascular permeability (leading to hypotension), cardiac function and induces metabolic changes culminating in tissue necrosis and organ failure. Consequently, the most common cause of mortality is acute kidney injury. The second phase (hypodynamic) is an anti-inflammatory process involving altered monocyte antigen presentation, decreased lymphocyte proliferation and function and increased apoptosis. This state known as immunosuppression or immune depression sharply increases the risk of nocosomial infections and ultimately, death. The mechanisms of these pathophysiological processes are not well characterized. Because both phases of sepsis may cause irreversible and irreparable damage, it is essential to determine the immunological and physiological status of the patient. This is the main reason why many therapeutic drugs have failed. The same drug given at different stages of sepsis may be therapeutic or otherwise harmful or have no effect 2,3. To understand sepsis at various levels it is crucial to have a suitable and comprehensive animal model that reproduces the clinical course of the disease. It is important to characterize the pathophysiological mechanisms occurring during sepsis and control the model conditions for testing potential therapeutic agents. 
To study the etiology of human sepsis researchers have developed different animal models. The most widely used clinical model is cecal ligation and puncture (CLP). The CLP model consists of the perforation of the cecum allowing the release of fecal material into the peritoneal cavity to generate an exacerbated immune response induced by polymicrobial infection. This model fulfills the human condition that is clinically relevant. As in humans, mice that undergo CLP with fluid resuscitation show the first (early) hyperdynamic phase that in time progresses to the second (late) hypodynamic phase. In addition, the cytokine profile is similar to that seen in human sepsis where there is increased lymphocyte apoptosis (reviewed in 4,5). Due to the multiple and overlapping mechanisms involved in sepsis, researchers need a suitable sepsis model of controlled severity in order to obtain consistent and reproducible results.

Watch this Scientific Journal Video about Cecal Ligation Puncture Procedure at JoVE.com

Cecal Ligation Puncture Procedure

cecal और मानव पूति के अध्ययन के लिए एक महत्वपूर्ण उपकरण के रूप में ligation पंचर के माउस मॉडल.

Cecal Ligation पंचर प्रक्रिया

Human sepsis is characterized by a set of systemic reactions in response to intensive and massive infection that failed to be locally contained by the ...

Research

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Medicine

Coronary Artery Ligation and Intramyocardial Injection in a Murine Model of Infarction

कोरोनरी धमनी Ligation और रोधगलन की एक murine मॉडल में Intramyocardial इंजेक्शन

Mouse models are a valuable tool for studying acute injury and chronic remodeling of the myocardium in vivo.  With the advent of genetic modifications to ...

The Application Of Permanent Middle Cerebral Artery Ligation in the Mouse

माउस में स्थायी मध्य सेरेब्रल धमनी Ligation के आवेदन

Focal cerebral ischemia is among the most common type of stroke seen in patients. Due to the clinical significance there has been a prolonged effort to ...

Modified Technique for Coronary Artery Ligation in Mice

चूहे में कोरोनरी धमनी ligation के लिए संशोधित तकनीक

Myocardial infarction (MI) is one of the most important causes of mortality in humans1-3. In order to improve morbidity and mortality in patients with MI ...

Murine Spinotrapezius Model to Assess the Impact of Arteriolar Ligation on Microvascular Function and Remodeling

Murine Spinotrapezius Microvascular फंक्शन और Remodeling पर Arteriolar Ligation के प्रभाव का आकलन मॉडल

The murine spinotrapezius is a thin, superficial skeletal support muscle that extends from T3 to L4, and is easily accessible via dorsal skin incision. ...

Bile Duct Ligation in Mice: Induction of Inflammatory Liver Injury and Fibrosis by Obstructive Cholestasis

चूहे में पित्त नली Ligation: ऑब्सट्रक्टिव पित्तस्थिरता द्वारा भड़काऊ जिगर चोट और फाइब्रोसिस की प्रेरण

In most vertebrates, the liver produces bile that is necessary to emulsify absorbed fats and enable the digestion of lipids in the small intestine as well ...

Reduction of Iatrogenic Atrial Septal Defects with an Anterior and Inferior Transseptal Puncture Site when Operating the Cryoballoon Ablation Catheter

एक पूर्वकाल और अवर Transseptal पंचर साइट के साथ चिकित्सकजनित एट्रियल सेप्टल दोष की कमी Cryoballoon एबलेशन कैथेटर जब ऑपरेटिंग

The cryoballoon catheter ablates atrial fibrillation (AF) triggers in the left atrium (LA) and pulmonary veins (PVs) via transseptal access. The typical ...

Murine Left Anterior Descending (LAD) Coronary Artery Ligation: An Improved and Simplified Model for Myocardial Infarction

Murine Left Anterior Descending LAD Coronary Artery Ligation: An Improved and Simplified Model for Myocardial Infarction

म्यूरीन वाम अवरोही धमनी (LAD) कोरोनरी धमनी Ligation: रोधगलन के लिए एक बेहतर और सरलीकृत मॉडल

Ischemic heart disease (IHD), or acute coronary syndrome (ACS), is one of the leading causes of death in the United States. IHD is characterized by ...

Rat Model of the Associating Liver Partition and Portal Vein Ligation for Staged Hepatectomy (ALPPS) Procedure

Rat Model of the Associating Liver Partition and Portal Vein Ligation for Staged Hepatectomy ALPPS Procedure

चूहा मॉडल के लिए संबद्ध जिगर विभाजन और पोर्टल नस बंधाव के मंचन के लिए Hepatectomy (ALPPS) प्रक्रिया

Recent clinical data support an aggressive surgical approach to both primary and metastatic liver tumors. For some indications, like colorectal liver ...

Puncture-Induced Iris Neovascularization as a Mouse Model of Rubeosis Iridis

Rubeosis Iridis के माउस मॉडल के रूप में पंचर-प्रेरित आइरिस Neovascularization

We describe a model of puncture-induced iris neovascularization as a general model for noninvasive evaluation of angiogenesis. The model is also relevant ...

Subclavian Vein Puncture As an Alternative Method of Blood Sample Collection in Rats

चूहों में रक्त नमूना संग्रह की एक वैकल्पिक विधि के रूप में अवजत्रुकी नस पंचर

Blood collection with enough blood volume is essential in animal experiments. Blood collection from the tail vein of rats is popular and less stressful ...