Name: intraluminal middle cerebral artery occlusion (mcao) model for ischemic stroke with laser doppler flowmetry guidance in mice
Uploaded: 2011-05-08T14:00:00
Description: Watch this Scientific Journal Video about Intraluminal Middle Cerebral Artery Occlusion MCAO Model for Ischemic Stroke with Laser Doppler Flowmetry Guidance in Mice at JoVE.com

该协议被批准的机构动物护理和使用委员会（IACUC）在佛罗里达大学，并遵守的“实验动物保健的原则”（美国国立卫生研究院出版号86-23，1985年修订）。 1。材料<ol><li>动物：雄性C57BL / 6小鼠（哈伦实验室，印第安纳波利斯，在美国）在手术时重达20-26克。 </li><li>麻醉： <ol><li>麻醉机：VetEquip吸入麻醉系统（VetEquip公司，Pleasanton的，CA，美国）。 </li><li>吸入麻醉药异氟醚（?...

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	<li>. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Recommendations+on+stroke+prevention,+diagnosis,+and+therapy.+Report+of+the+WHO+Task+Force+on+Stroke+and+other+Cerebrovascular+Disorders.">Recommendations on stroke prevention, diagnosis, and therapy. 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MCAO模型是一个既定的模式，复制脑缺血小鼠物种。第一次使用这个模型是在大鼠和以后适应小鼠。避免去骨瓣，以消除对血脑屏障通透性和颅内压手术操作的影响，是该技术的优点。然而，许多变量，如应变相关的差异 10，大小缝合技巧 11，持续时间 6闭塞， 体温 12， 麻醉 13，以及其他因素，可能导致梗塞体积在这个模型不一致。 值得一提的有具?...

<table border="1"><tbody><tr><th>试剂名称</th><th>类型</th><th>公司</th><th>目录编号</th><th>评论（可选） </th></tr><tr><td>雄性C57BL / 6小鼠</td><td>动物</td><td>哈伦实验室，印第安纳波利斯，在美国</td><td></td><td> 20-26 GRS </td></tr><tr><td>解剖镜下</td><td>显微镜</td><td>徕卡立体显微镜MZ6 </td><td></td><td> 6.3:1变焦</td></tr><tr><td>光纤光纤照明</td><td>照明灯</td><td> TechniQuip公司，利弗莫尔，CA，美国</td><td></td><td> FO1 - 150 </td></tr><tr><td>异氟醚</td><td>麻醉</td><td>巴克斯特制药，迪尔菲尔德，白细胞介素</td><td> 1001936060 </td><td></td></tr><tr><td> SuperCut虹膜剪，直</td><td>手术工具</td><td>世界精密仪器公司，美国，佛罗里达州，萨拉索塔</td><td> 14218 </td><td></td></tr><tr><td> Vannas微，剪刀，直</td><td>手术工具</td><td>世界精密仪器公司，美国，佛罗里达州，萨拉索塔</td><td> 14003 </td><td></td></tr><tr><td>杜蒙细尖镊子</td><td>手术工具</td><td>世界精密仪器公司，美国，佛罗里达州，萨拉索塔</td><td> 503290 </td><td></td></tr><tr><td>血管夹</td><td>手术工具</td><td>世界精密仪器公司，美国，佛罗里达州，萨拉索塔</td><td> 14120 </td><td></td></tr><tr><td>高频干燥器900 </td><td>手术工具</td><td>世界精密仪器公司，美国，佛罗里达州，萨拉索塔</td><td> 500397 </td><td></td></tr><tr><td> 7-0手术尼龙单丝缝合</td><td>缝合</td><td>爱惜康公司，美国，新泽西州，的萨默维尔</td><td> 1647G </td><td>缝合闭塞</td></tr><tr><td> 7-0手术丝线缝合</td><td>缝合</td><td>爱惜康公司，美国，新泽西州，的萨默维尔</td><td> 7733G </td><td>缝合结扎</td></tr><tr><td>有机硅室温胶</td><td>硅橡胶</td><td>世界精密仪器公司，美国，佛罗里达州，萨拉索塔</td><td> 1571 </td><td></td></tr><tr><td> Vetbond </td><td>组织粘合剂</td><td> 3M公司，美国明尼苏达州圣保罗， </td><td> 70200746587 </td><td></td></tr><tr><td> Puralube兽医软膏</td><td>眼膏</td><td> Pharmaderm，梅尔维尔，纽约，美国</td><td></td><td></td></tr><tr><td>丁丙诺啡hydrochlorode </td><td>止痛药</td><td> Hospira公司公司，森林湖，IL，美国</td><td> NDC 0409-2012-32 </td><td></td></tr><tr><td>热烧灼组</td><td></td><td>世界精密仪器公司，美国，佛罗里达州，萨拉索塔</td><td> 501292 </td><td></td></tr><tr><td> PeriFlux系统5000 </td><td>经颅多普勒血流</td><td> Perimed，斯德哥尔摩，Sweeden </td><td></td><td></td></tr><tr><td> BAT - 12 </td><td>直肠温度计</td><td>世界精密仪器公司，美国，佛罗里达州，萨拉索塔</td><td>英美烟草- 12R </td><td></td></tr><tr><td> T /泵，TP600 </td><td>隔热毯</td><td>美国，纽约，Gaymar </td><td></td><td></td></tr><tr><td> 2,3,5 - 三苯基氯化</td><td>试剂</td><td> Sigma - Aldrich公司有限公司，美国密苏里州圣路易斯， </td><td> T8877 </td><td></td></tr><tr><td>小鼠脑矩阵切片机</td><td>脑切片</td><td> Zivic米勒实验室，公司艾里逊公园，PA，美国</td><td> BSMAS001 - 1 </td><td></td></tr></tbody></table>

intraluminal middle cerebral artery occlusion (mcao) model for ischemic stroke with laser doppler flowmetry guidance in mice

中风是第三大死因，在世界上的残疾的首要原因，美国在2009 年 1,2的近70亿美元，估计所需费用。腔内大脑中动脉闭塞（MCAO）模型，于1986年，以模拟在大鼠有影响力的人类病理学由小泉纯一郎 4 。缺血法的修改后由龙格 3 。这两种技术已被广泛用来识别脑损伤的分子机制，导致缺血性中风和潜在的治疗方式5。一直延续这相对无创的方法，在大鼠，小鼠的使用，利用转基因和基因敲除株6,7优势。局灶性脑缺血模型，线栓是先进的经颈内动脉闭塞MCA的基地。回缩后，在指定的时间内缝合模仿自发再灌注损伤，但缝合，也可永久保留。该视频将展示在小鼠进行腔内缺血过程在逐步时尚的两个主要方法，以及为潜在的弊端和缺陷的见解。缺血性脑组织也将随之由2,3,5 -三苯基氯化四氮唑（TTC）染色，评估脑梗塞 8的程度。

Watch this Scientific Journal Video about Intraluminal Middle Cerebral Artery Occlusion MCAO Model for Ischemic Stroke with Laser Doppler Flowmetry Guidance in Mice at JoVE.com

Intraluminal Middle Cerebral Artery Occlusion MCAO Model for Ischemic Stroke with Laser Doppler Flowmetry Guidance in Mice

腔内大脑中动脉闭塞（MCAO）模型是最常见的缺血性中风的实验模型中使用的模型。在这里，我们将展示在整个模型用激光多普勒血流计指南的细节，其代表性的成果。

腔内中东大脑中动脉闭塞（MCAO）缺血性中风模型在小鼠体内的激光多普勒血流指导

Stroke is the third leading cause of death and the leading cause of disability in the world, with an estimated cost of near $70 billion 
in the United ...

Research

JoVE Journal

Medicine

Intraluminal Middle Cerebral Artery Occlusion (MCAO) Model for Ischemic Stroke with Laser Doppler Flowmetry Guidance in Mice

Modeling Stroke in Mice - Middle Cerebral Artery Occlusion with the Filament Model

建模在小鼠体内的行程 - 中东与灯丝模型大脑中动脉闭塞

Stroke is among the most frequent causes of death and adult disability, especially in highly developed countries. However, treatment options to date are ...

科研

医学

Mouse Model of Middle Cerebral Artery Occlusion

大脑中动脉闭塞的小鼠模型

Stroke is the most common fatal neurological disease in the United States 1. The majority of strokes (88%) result from blockage of blood vessels in the ...

Bilateral Common Carotid Artery Occlusion as an Adequate Preconditioning Stimulus to Induce Early Ischemic Tolerance to Focal Cerebral Ischemia

双侧颈总动脉闭塞足够的预处理刺激诱导局灶性脑缺血早期缺血耐受

There is accumulating evidence, that ischemic preconditioning - a non-damaging ischemic challenge to the brain - confers a transient protection to a ...

Endothelin-1 Induced Middle Cerebral Artery Occlusion Model for Ischemic Stroke with Laser Doppler Flowmetry Guidance in Rat

内皮素-1诱导大脑中动脉闭塞模型缺血性中风激光多普勒血流仪在大鼠指导

Stroke is the number one cause of disability and third leading cause of death in the world, costing an estimated $70 billion in the United States in ...

Utilizing a Cranial Window to Visualize the Middle Cerebral Artery During Endothelin-1 Induced Middle Cerebral Artery Occlusion

利用颅窗口，在可视化的大脑中动脉内皮素-1诱导大脑中动脉闭塞

Creation of a cranial window is a method that allows direct visualization of structures on the cortical surface of the brain1-3. This technique can be ...

Optimized System for Cerebral Perfusion Monitoring in the Rat Stroke Model of Intraluminal Middle Cerebral Artery Occlusion

优化系统的脑灌注监测在中风模型大鼠的大脑中动脉闭塞腔内

The translational potential of pre-clinical stroke research depends on the accuracy of experimental modeling. Cerebral perfusion monitoring in animal ...

Modeling Stroke in Mice: Permanent Coagulation of the Distal Middle Cerebral Artery

造型中风小鼠：在远端大脑中动脉永久性混凝

Stroke is the third most common cause of death and a main cause of acquired adult disability in developed countries. Only very limited therapeutical ...

Embolic Middle Cerebral Artery Occlusion (MCAO) for Ischemic Stroke with Homologous Blood Clots in Rats

Embolic Middle Cerebral Artery Occlusion MCAO for Ischemic Stroke with Homologous Blood Clots in Rats

Clinically, thrombolytic therapy with use of recombinant tissue plasminogen activator (tPA) remains the most effective treatment for acute ischemic ...

Quantification of Neurovascular Protection Following Repetitive Hypoxic Preconditioning and Transient Middle Cerebral Artery Occlusion in Mice

神经血管保护定量继在小鼠重复缺氧预处理和瞬态大脑中动脉闭塞

Experimental animal models of stroke are invaluable tools for understanding stroke pathology and developing more effective treatment strategies. A 2 week ...

Performing Permanent Distal Middle Cerebral with Common Carotid Artery Occlusion in Aged Rats to Study Cortical Ischemia with Sustained Disability

执行永久远端大脑中与老年大鼠颈总动脉闭塞研究皮层缺血具有持续残疾

Stroke typically occurs in elderly people with a range of comorbidities including carotid (or other arterial) atherosclerosis, high blood pressure, ...