Name: intraluminal middle cerebral artery occlusion (mcao) model for ischemic stroke with laser doppler flowmetry guidance in mice
Uploaded: 2011-05-08T14:00:00
Description: Watch this Scientific Journal Video about Intraluminal Middle Cerebral Artery Occlusion MCAO Model for Ischemic Stroke with Laser Doppler Flowmetry Guidance in Mice at JoVE.com

Dit protocol werd goedgekeurd door de Institutional Animal Care en gebruik Comite (IACUC) aan de Universiteit van Florida en is in overeenstemming met het &quot;beginsel van Laboratory Animal Care&quot; (NIH publicatie No 86-23, herzien 1985). 1. Materialen <ol><li> Dieren: Man C57BL / 6 muizen (Harlan Laboratories, Indianapolis, IN, USA) met een gewicht van 20-26 gram op het moment van de operatie. </li><li> Anesthesie: <ol><li> Anesthesieapparaat: VetEquip inhalatie-anesthesie-systeem (V...

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	<li>. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Recommendations+on+stroke+prevention,+diagnosis,+and+therapy.+Report+of+the+WHO+Task+Force+on+Stroke+and+other+Cerebrovascular+Disorders.">Recommendations on stroke prevention, diagnosis, and therapy. Report of the WHO Task Force on Stroke and other Cerebrovascular Disorders.</a> Stroke; a journal of cerebral circulation. 20, 1407-1431 (1989).</li><li>Lloyd-Jones, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Heart+disease+and+stroke+statistics--2009+update:+a+report+from+the+American+Heart+Association+Statistics+Committee+and+Stroke+Statistics+Subcommittee.">Heart disease and stroke statistics--2009 update: a report from the American Heart Association Statistics Committee and Stroke Statistics Subcommittee.</a> Circulation. 119, e21-e181 (2009).</li><li>Longa, E. Z., Weinstein, P. 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P., Eckenstein, F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Monofilament+intraluminal+middle+cerebral+artery+occlusion+in+the+mouse.">Monofilament intraluminal middle cerebral artery occlusion in the mouse.</a> Neurol Res. 19, 641-648 (1997).</li><li>Fujii, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Strain-related+differences+in+susceptibility+to+transient+forebrain+ischemia+in+SV-129+and+C57black/6+mice.+.">Strain-related differences in susceptibility to transient forebrain ischemia in SV-129 and C57black/6 mice. .</a> Stroke; a journal of cerebral circulation. 28, 1805-1810 (1997).</li><li>Bederson, J. 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N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mouse+strain+differences+in+susceptibility+to+cerebral+ischemia+are+related+to+cerebral+vascular+anatomy.">Mouse strain differences in susceptibility to cerebral ischemia are related to cerebral vascular anatomy.</a> J Cereb Blood Flow Metab. 13, 683-692 (1993).</li><li>Tureyen, K., Vemuganti, R., Sailor, K. A., Dempsey, R. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Ideal+suture+diameter+is+critical+for+consistent+middle+cerebral+artery+occlusion+in+mice.">Ideal suture diameter is critical for consistent middle cerebral artery occlusion in mice.</a> Neurosurgery. 56, 196-200 (2005).</li><li>Barber, P. A., Hoyte, L., Colbourne, F., Buchan, A. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Temperature-regulated+model+of+focal+ischemia+in+the+mouse:+a+study+with+histopathological+and+behavioral+outcomes.">Temperature-regulated model of focal ischemia in the mouse: a study with histopathological and behavioral outcomes.</a> Stroke; a journal of cerebral circulation. 35, 1720-1725 (2004).</li><li>Kapinya, K. J., Prass, K., Dirnagl, U. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Isoflurane+induced+prolonged+protection+against+cerebral+ischemia+in+mice:+a+redox+sensitive+mechanism?.">Isoflurane induced prolonged protection against cerebral ischemia in mice: a redox sensitive mechanism?.</a> Neuroreport. 13, 1431-1435 (2002).</li></ol>

Het MCAO model is een model dat vastgesteld cerebrale ischemie repliceert in muriene soorten. Dit model werd voor het eerst gebruikt in ratten en daarna aangepast aan de muizen. Het vermijden van craniectomy om de invloed van chirurgische manipulatie op de bloed-hersen barrière permeabiliteit en intracraniale druk te elimineren is een voordeel van deze techniek. Echter, veel variabelen, zoals de stam-gerelateerde verschillen 10, de grootte van hechtdraad tip 11, duur van de occlusie 6, ...

<table border="1"><tbody><tr><th> Naam van het serum </th><th> Type </th><th> Vennootschap </th><th> Catalogus nummer </th><th> Commentaar (optioneel) </th></tr><tr><td> Man C57BL / 6 muizen </td><td> Dier </td><td> Harlan Laboratories, Indianapolis, IN, USA </td><td></td><td> 20-26 grs </td></tr><tr><td> Dissection microscoop </td><td> Microscoop </td><td> Leica stereo microscoop MZ6 </td><td></td><td> 6.3:1 zoom </td></tr><tr><td> Fiber optic verlichting </td><td> Illuminator </td><td> TechniQuip Corp, Livermore, CA, USA </td><td></td><td> FO1-150 </td></tr><tr><td> Isofluraan </td><td> Anesthesie </td><td> Baxter Pharmaceutics, Deerfield, IL </td><td> 1001936060 </td><td></td></tr><tr><td> SuperCut Iris schaar, recht </td><td> Chirurgisch instrument </td><td> Wereld precisie-instrumenten, Inc, Sarasota, FL, USA </td><td> 14218 </td><td></td></tr><tr><td> Vannas micro-schaar, recht </td><td> Chirurgisch instrument </td><td> Wereld precisie-instrumenten, Inc, Sarasota, FL, USA </td><td> 14003 </td><td></td></tr><tr><td> Dumont fijne punt tang </td><td> Chirurgisch instrument </td><td> Wereld precisie-instrumenten, Inc, Sarasota, FL, USA </td><td> 503290 </td><td></td></tr><tr><td> Vessel clip </td><td> Chirurgisch instrument </td><td> Wereld precisie-instrumenten, Inc, Sarasota, FL, USA </td><td> 14120 </td><td></td></tr><tr><td> High Frequency Exsiccator 900 </td><td> Chirurgisch instrument </td><td> Wereld precisie-instrumenten, Inc, Sarasota, FL, USA </td><td> 500397 </td><td></td></tr><tr><td> 7-0 chirurgische Nylon monofilament hechtmateriaal </td><td> Hechting </td><td> Ethicon, Inc, Somerville, NJ, USA </td><td> 1647G </td><td> Suture voor occlusie </td></tr><tr><td> 7-0 chirurgisch hechtdraad Silk </td><td> Hechting </td><td> Ethicon, Inc, Somerville, NJ, USA </td><td> 7733G </td><td> Suture voor ligatie </td></tr><tr><td> Siliconen RTV lijm </td><td> Silicone rubber </td><td> Wereld precisie-instrumenten, Inc, Sarasota, FL, USA </td><td> 1571 </td><td></td></tr><tr><td> Vetbond </td><td> Tissue lijm </td><td> 3M Company, St. Paul, MN, USA </td><td> 70200746587 </td><td></td></tr><tr><td> Puralube Vet Zalf </td><td> Oogzalf </td><td> Pharmaderm, Melville, NY, USA </td><td></td><td></td></tr><tr><td> Buprenorfine hydrochlorode </td><td> Analgetica </td><td> Hospira Inc, Lake Forest, Illinois, USA </td><td> NDC 0409-2012-32 </td><td></td></tr><tr><td> Thermische cauterisatie Unit </td><td></td><td> Wereld precisie-instrumenten, Inc, Sarasota, FL, USA </td><td> 501292 </td><td></td></tr><tr><td> PeriFlux System 5000 </td><td> TCD flowmetry </td><td> Perimed, Stockholm, Sweeden </td><td></td><td></td></tr><tr><td> BAT-12 </td><td> Rectale thermometer </td><td> Wereld precisie-instrumenten, Inc, Sarasota, FL, USA </td><td> BAT-12R </td><td></td></tr><tr><td> T / POMP, TP600 </td><td> Thermische deken </td><td> Gaymar, NY, USA </td><td></td><td></td></tr><tr><td> 2,3,5-triphenyltetrazolium chloride </td><td> Reagens </td><td> Sigma-Aldrich Co, St Louis, MO, USA </td><td> T8877 </td><td></td></tr><tr><td> Muizen hersenen matrix slicer </td><td> Brain slicer </td><td> Zivic-Miller Lab., Inc, Allison Park, PA, USA </td><td> BSMAS001-1 </td><td></td></tr></tbody></table>

intraluminal middle cerebral artery occlusion (mcao) model for ischemic stroke with laser doppler flowmetry guidance in mice

Beroerte is de derde doodsoorzaak en de belangrijkste oorzaak van invaliditeit in de wereld, met een geschatte kost van bijna 70 miljard dollar in de Verenigde Staten in 2009 1,2. De intraluminale midden cerebrale slagader occlusie (MCAO) model is ontwikkeld door vier Koizumi in 1986 aan deze indrukwekkende menselijke pathologie te simuleren in de rat. Een wijziging van de MCAO methode werd later door Longa 3. Beide technieken zijn op grote schaal gebruikt om de moleculaire mechanismen van hersenletsel als gevolg van ischemische beroerte en potentiële therapeutische modaliteiten 5 te identificeren. Deze relatief niet-invasieve methode bij ratten is uitgebreid om te gebruiken in muizen om te profiteren van transgene en knock-out stammen 6,7. Voor het modelleren van focale cerebrale ischemie, is een intraluminale hechtdraad geavanceerde via de interne halsslagader aan de basis van de MCA af te sluiten. Het terugtrekken van de hechting na een bepaalde periode nabootst spontane reperfusie, maar de hechting kan ook permanent worden bewaard. Deze video demonstreert de twee grote benaderingen voor het uitvoeren van intraluminale MCAO procedure bij muizen op een stapsgewijze manier, alsmede het verschaffen van inzichten voor potentiële nadelen en valkuilen. De ischemische hersenweefsel wordt vervolgens gekleurd door 2,3,5-triphenyltetrazolium chloride (TTC) om de mate van herseninfarct acht te evalueren.

Watch this Scientific Journal Video about Intraluminal Middle Cerebral Artery Occlusion MCAO Model for Ischemic Stroke with Laser Doppler Flowmetry Guidance in Mice at JoVE.com

Intraluminal Middle Cerebral Artery Occlusion MCAO Model for Ischemic Stroke with Laser Doppler Flowmetry Guidance in Mice

De intraluminale midden cerebrale slagader occlusie (MCAO) model is de meest frequente gebruikte model onder experimentele ischemische beroerte modellen. Hier zullen we demonstreren het gehele model in detail met de gids van Laser Doppler flowmetry, en zijn vertegenwoordiger resultaten.

Intraluminale midden cerebrale slagader Occlusie (MCAO) Model voor ischemische beroerte met Laser Doppler Flowmetry Begeleiding in Muizen

Stroke is the third leading cause of death and the leading cause of disability in the world, with an estimated cost of near $70 billion 
in the United ...

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Medicine

Intraluminal Middle Cerebral Artery Occlusion (MCAO) Model for Ischemic Stroke with Laser Doppler Flowmetry Guidance in Mice

Modeling Stroke in Mice - Middle Cerebral Artery Occlusion with the Filament Model

Modellering Stroke in Muizen - midden cerebrale slagader occlusie met de gloeidraad Model

Stroke is among the most frequent causes of death and adult disability, especially in highly developed countries. However, treatment options to date are ...

Mouse Model of Middle Cerebral Artery Occlusion

Muis model van de midden cerebrale slagader Occlusion

Stroke is the most common fatal neurological disease in the United States 1. The majority of strokes (88%) result from blockage of blood vessels in the ...

Bilateral Common Carotid Artery Occlusion as an Adequate Preconditioning Stimulus to Induce Early Ischemic Tolerance to Focal Cerebral Ischemia

Bilaterale Gemeenschappelijke occlusie van de halsslagader als een adequate Voorconditionering Stimulus naar Early ischemische tolerantie te induceren bij focale ischemie

There is accumulating evidence, that ischemic preconditioning - a non-damaging ischemic challenge to the brain - confers a transient protection to a ...

Endothelin-1 Induced Middle Cerebral Artery Occlusion Model for Ischemic Stroke with Laser Doppler Flowmetry Guidance in Rat

Endotheline-1 geïnduceerde midden cerebrale slagader occlusie Model voor ischemische beroerte met Laser Doppler flowmetrie Begeleiding in Rat

Stroke is the number one cause of disability and third leading cause of death in the world, costing an estimated $70 billion in the United States in ...

Utilizing a Cranial Window to Visualize the Middle Cerebral Artery During Endothelin-1 Induced Middle Cerebral Artery Occlusion

Met behulp van een Craniale venster om de midden cerebrale slagader Visualiseer Tijdens Endotheline-1 geïnduceerde midden cerebrale slagader occlusie

Creation of a cranial window is a method that allows direct visualization of structures on the cortical surface of the brain1-3. This technique can be ...

Optimized System for Cerebral Perfusion Monitoring in the Rat Stroke Model of Intraluminal Middle Cerebral Artery Occlusion

Geoptimaliseerde Systeem voor cerebrale perfusie Monitoring in het Rat Stroke Model van intraluminale midden cerebrale slagader occlusie

The translational potential of pre-clinical stroke research depends on the accuracy of experimental modeling. Cerebral perfusion monitoring in animal ...

Modeling Stroke in Mice: Permanent Coagulation of the Distal Middle Cerebral Artery

Modellering Stroke in Muizen: Permanente Coagulatie van de distale midden cerebrale slagader

Stroke is the third most common cause of death and a main cause of acquired adult disability in developed countries. Only very limited therapeutical ...

Embolic Middle Cerebral Artery Occlusion (MCAO) for Ischemic Stroke with Homologous Blood Clots in Rats

Embolic Middle Cerebral Artery Occlusion MCAO for Ischemic Stroke with Homologous Blood Clots in Rats

Embolische midden cerebrale slagader occlusie (MCAO) voor ischemische beroerte met homologe bloedstolsels in Ratten

Clinically, thrombolytic therapy with use of recombinant tissue plasminogen activator (tPA) remains the most effective treatment for acute ischemic ...

Quantification of Neurovascular Protection Following Repetitive Hypoxic Preconditioning and Transient Middle Cerebral Artery Occlusion in Mice

Kwantificering van Neurovasculaire Protection Na Repetitive Hypoxische Voorconditionering en Transient midden cerebrale slagader Occlusie in Muizen

Experimental animal models of stroke are invaluable tools for understanding stroke pathology and developing more effective treatment strategies. A 2 week ...

Performing Permanent Distal Middle Cerebral with Common Carotid Artery Occlusion in Aged Rats to Study Cortical Ischemia with Sustained Disability

Het uitvoeren van permanente distale midden cerebrale met Common occlusie van de halsslagader in oude ratten te studeren Cortical ischemie met Aanhoudende Disability

Stroke typically occurs in elderly people with a range of comorbidities including carotid (or other arterial) atherosclerosis, high blood pressure, ...