Name: intraluminal middle cerebral artery occlusion (mcao) model for ischemic stroke with laser doppler flowmetry guidance in mice
Uploaded: 2011-05-08T14:00:00
Description: Watch this Scientific Journal Video about Intraluminal Middle Cerebral Artery Occlusion MCAO Model for Ischemic Stroke with Laser Doppler Flowmetry Guidance in Mice at JoVE.com

Questo protocollo è stato approvato dalla cura degli animali e utilizzo Comitato Istituzionale (IACUC) presso l&#39;Università della Florida ed è conforme con il &quot;Principio di prodotti per animali da laboratorio&quot; (NIH pubblicazione n ° 86-23, riveduta 1985). 1. Materiale <ol><li> Animali: maschio topi C57BL / 6 (Laboratori di Harlan, Indianapolis, IN, USA) pesa 20-26 grammi al momento della chirurgia. </li><li> Anestesia: <ol><li> Anestesia macchina: sistema di inalazione Vet...

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	<li>. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Recommendations+on+stroke+prevention,+diagnosis,+and+therapy.+Report+of+the+WHO+Task+Force+on+Stroke+and+other+Cerebrovascular+Disorders.">Recommendations on stroke prevention, diagnosis, and therapy. Report of the WHO Task Force on Stroke and other Cerebrovascular Disorders.</a> Stroke; a journal of cerebral circulation. 20, 1407-1431 (1989).</li><li>Lloyd-Jones, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Heart+disease+and+stroke+statistics--2009+update:+a+report+from+the+American+Heart+Association+Statistics+Committee+and+Stroke+Statistics+Subcommittee.">Heart disease and stroke statistics--2009 update: a report from the American Heart Association Statistics Committee and Stroke Statistics Subcommittee.</a> Circulation. 119, e21-e181 (2009).</li><li>Longa, E. Z., Weinstein, P. R., Carlson, S., Cummins, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Reversible+middle+cerebral+artery+occlusion+without+craniectomy+in+rats.">Reversible middle cerebral artery occlusion without craniectomy in rats.</a> Stroke; a journal of cerebral circulation. 20, 84-91 (1989).</li><li>Koizumi, J., Yoshida, Y., Nakazawa, T., Ooneda, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Experimental+studies+of+ischemic+brain+edema.+I.+A+new+experimental+model+of+cerebral+embolism+in+rats+in+which+recirculation+can+be+introduced+in+the+ischemic+area.">Experimental studies of ischemic brain edema. I. A new experimental model of cerebral embolism in rats in which recirculation can be introduced in the ischemic area.</a> Jpn J Stroke. 8, 1-8 (1986).</li><li>Clark, W. M., Lessov, N. S., Dixon, M. P., Eckenstein, F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Monofilament+intraluminal+middle+cerebral+artery+occlusion+in+the+mouse.">Monofilament intraluminal middle cerebral artery occlusion in the mouse.</a> Neurol Res. 19, 641-648 (1997).</li><li>Fujii, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Strain-related+differences+in+susceptibility+to+transient+forebrain+ischemia+in+SV-129+and+C57black/6+mice.+.">Strain-related differences in susceptibility to transient forebrain ischemia in SV-129 and C57black/6 mice. .</a> Stroke; a journal of cerebral circulation. 28, 1805-1810 (1997).</li><li>Bederson, J. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Evaluation+of+2,3,5-triphenyltetrazolium+chloride+as+a+stain+for+detection+and+quantification+of+experimental+cerebral+infarction+in+rats.">Evaluation of 2,3,5-triphenyltetrazolium chloride as a stain for detection and quantification of experimental cerebral infarction in rats.</a> Stroke; a journal of cerebral circulation. 17, 1304-1308 (1986).</li><li>Qi, X., Hosoi, T., Okuma, Y., Kaneko, M., Nomura, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Sodium+4-phenylbutyrate+protects+against+cerebral+ischemic+injury.">Sodium 4-phenylbutyrate protects against cerebral ischemic injury.</a> Mol Pharmacol. 66, 899-908 (2004).</li><li>Barone, F. C., Knudsen, D. J., Nelson, A. H., Feuerstein, G. Z., Willette, R. N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mouse+strain+differences+in+susceptibility+to+cerebral+ischemia+are+related+to+cerebral+vascular+anatomy.">Mouse strain differences in susceptibility to cerebral ischemia are related to cerebral vascular anatomy.</a> J Cereb Blood Flow Metab. 13, 683-692 (1993).</li><li>Tureyen, K., Vemuganti, R., Sailor, K. A., Dempsey, R. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Ideal+suture+diameter+is+critical+for+consistent+middle+cerebral+artery+occlusion+in+mice.">Ideal suture diameter is critical for consistent middle cerebral artery occlusion in mice.</a> Neurosurgery. 56, 196-200 (2005).</li><li>Barber, P. A., Hoyte, L., Colbourne, F., Buchan, A. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Temperature-regulated+model+of+focal+ischemia+in+the+mouse:+a+study+with+histopathological+and+behavioral+outcomes.">Temperature-regulated model of focal ischemia in the mouse: a study with histopathological and behavioral outcomes.</a> Stroke; a journal of cerebral circulation. 35, 1720-1725 (2004).</li><li>Kapinya, K. J., Prass, K., Dirnagl, U. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Isoflurane+induced+prolonged+protection+against+cerebral+ischemia+in+mice:+a+redox+sensitive+mechanism?.">Isoflurane induced prolonged protection against cerebral ischemia in mice: a redox sensitive mechanism?.</a> Neuroreport. 13, 1431-1435 (2002).</li></ol>

Il modello MCAO è un modello stabilito che replica ischemia cerebrale in specie murina. Questo modello è stato utilizzato nel ratto e successivamente adattato alle topi. Evitare craniectomia per eliminare l&#39;influenza di manipolazione chirurgica sulla permeabilità della barriera emato-encefalica e della pressione intracranica è un vantaggio di questa tecnica. Tuttavia, molte variabili, come il ceppo differenze legate al 10, dimensioni della punta di sutura 11, durata dell&#39;occlusione <sup...

<table border="1"><tbody><tr><th> Nome del reagente </th><th> Tipo </th><th> Azienda </th><th> Numero di catalogo </th><th> Commenti (opzionale) </th></tr><tr><td> Maschio C57BL / 6 topi </td><td> Animale </td><td> Laboratori di Harlan, Indianapolis, IN, USA </td><td></td><td> 20-26 gr </td></tr><tr><td> Dissezione microscopio </td><td> Microscopio </td><td> Leica stereo microscopio MZ6 </td><td></td><td> 6.3:1 zoom </td></tr><tr><td> Illuminatore in fibra ottica </td><td> Illuminatore </td><td> TechniQuip Corp., Livermore, CA, USA </td><td></td><td> FO1-150 </td></tr><tr><td> Isoflurano </td><td> Anestesia </td><td> Farmaceutica Baxter, Deerfield, IL </td><td> 1001936060 </td><td></td></tr><tr><td> SuperCut Iris forbice, diritto </td><td> Strumento chirurgico </td><td> Mondo Precision Instruments, Inc., Sarasota, FL, USA </td><td> 14218 </td><td></td></tr><tr><td> Vännäs micro-forbice, diritto </td><td> Strumento chirurgico </td><td> Mondo Precision Instruments, Inc., Sarasota, FL, USA </td><td> 14003 </td><td></td></tr><tr><td> Pinze punta Dumont multa </td><td> Strumento chirurgico </td><td> Mondo Precision Instruments, Inc., Sarasota, FL, USA </td><td> 503290 </td><td></td></tr><tr><td> Clip nave </td><td> Strumento chirurgico </td><td> Mondo Precision Instruments, Inc., Sarasota, FL, USA </td><td> 14120 </td><td></td></tr><tr><td> Essiccatore ad alta frequenza 900 </td><td> Strumento chirurgico </td><td> Mondo Precision Instruments, Inc., Sarasota, FL, USA </td><td> 500397 </td><td></td></tr><tr><td> 7-0 sutura chirurgica nylon monofilamento </td><td> Sutura </td><td> Ethicon, Inc., Somerville, NJ, USA </td><td> 1647G </td><td> Sutura per l&#39;occlusione </td></tr><tr><td> 7-0 sutura chirurgica della Seta </td><td> Sutura </td><td> Ethicon, Inc., Somerville, NJ, USA </td><td> 7733G </td><td> Sutura di legatura </td></tr><tr><td> Silicone RTV adesivo </td><td> Gomma siliconica </td><td> Mondo Precision Instruments, Inc., Sarasota, FL, USA </td><td> 1571 </td><td></td></tr><tr><td> Vetbond </td><td> Tessuto adesivo </td><td> 3M Company, St. Paul, MN, USA </td><td> 70200746587 </td><td></td></tr><tr><td> Puralube Vet Unguento </td><td> Occhio unguento </td><td> Pharmaderm, Melville, NY, USA </td><td></td><td></td></tr><tr><td> Buprenorfina hydrochlorode </td><td> Analgesici </td><td> Hospira Inc., Lake Forest, Illinois, USA </td><td> NDC 0409-2012-32 </td><td></td></tr><tr><td> Cauterio unità termica </td><td></td><td> Mondo Precision Instruments, Inc., Sarasota, FL, USA </td><td> 501292 </td><td></td></tr><tr><td> PeriFlux System 5000 </td><td> TCD flussimetria </td><td> Perimed, Stoccolma, Svezia </td><td></td><td></td></tr><tr><td> BAT-12 </td><td> Termometro rettale </td><td> Mondo Precision Instruments, Inc., Sarasota, FL, USA </td><td> BAT-12R </td><td></td></tr><tr><td> T / POMPA, TP600 </td><td> Coperta termica </td><td> Gaymar, NY, USA </td><td></td><td></td></tr><tr><td> 2,3,5-trifeniltetrazolio cloruro </td><td> Reagente </td><td> Sigma-Aldrich, St. Louis, MO, USA </td><td> T8877 </td><td></td></tr><tr><td> I topi cervello matrice affettatrice </td><td> Cervello affettatrice </td><td> Zivic Miller-Lab. Inc., Allison Park, PA, USA </td><td> BSMAS001-1 </td><td></td></tr></tbody></table>

intraluminal middle cerebral artery occlusion (mcao) model for ischemic stroke with laser doppler flowmetry guidance in mice

L&#39;ictus è la terza causa di morte e la principale causa di disabilità nel mondo, con un costo stimato di quasi 70 miliardi dollari negli Stati Uniti nel 2009 1,2. Il intraluminale occlusione dell&#39;arteria cerebrale media (MCAO) il modello è stato sviluppato da Koizumi 4 nel 1986 per simulare questa patologia di forte impatto umano nel ratto. Una modifica del metodo di MCAO è stato poi presentato dalla Longa 3. Entrambe le tecniche sono stati ampiamente utilizzati per identificare i meccanismi molecolari delle lesioni cerebrali da ictus ischemico e potenziali modalità terapeutiche 5. Questo metodo relativamente non invasivo nei ratti è stata estesa da utilizzare nei topi per sfruttare ceppi transgenici e knockout 6,7. Per modello di ischemia cerebrale focale, una sutura intraluminale è avanzato attraverso l&#39;arteria carotide interna per occludere la base del MCA. Ritraendo la sutura dopo un determinato periodo di tempo imita riperfusione spontanea, ma la sutura può essere mantenuto in permanenza. Questo video sarà dimostrare i due principali approcci per l&#39;esecuzione di intraluminale procedura MCAO nei topi in modo graduale, oltre a fornire spunti per potenziali svantaggi e le insidie. Il tessuto cerebrale ischemico sarà successivamente colorate con 2,3,5-trifeniltetrazolio cloruro (TTC) per valutare il grado di infarto cerebrale 8.

Watch this Scientific Journal Video about Intraluminal Middle Cerebral Artery Occlusion MCAO Model for Ischemic Stroke with Laser Doppler Flowmetry Guidance in Mice at JoVE.com

Intraluminal Middle Cerebral Artery Occlusion MCAO Model for Ischemic Stroke with Laser Doppler Flowmetry Guidance in Mice

Il intraluminale occlusione dell&#39;arteria cerebrale media (MCAO) modello è il modello più frequente tra i modelli sperimentali utilizzati ictus ischemico. Qui dimostrerà tutto il modello in dettaglio con la guida di flussimetria laser Doppler, ed i suoi risultati rappresentativi.

Intraluminali occlusione dell&#39;arteria cerebrale media (MCAO) Modello per l&#39;ictus ischemico con la guida flussimetria laser Doppler in topi

Stroke is the third leading cause of death and the leading cause of disability in the world, with an estimated cost of near $70 billion 
in the United ...

Research

JoVE Journal

Medicine

Intraluminal Middle Cerebral Artery Occlusion (MCAO) Model for Ischemic Stroke with Laser Doppler Flowmetry Guidance in Mice

Modeling Stroke in Mice - Middle Cerebral Artery Occlusion with the Filament Model

Corsa di modellazione in Mouse - Medio occlusione dell&#39;arteria cerebrale con il Modello Filament

Stroke is among the most frequent causes of death and adult disability, especially in highly developed countries. However, treatment options to date are ...

Ricerca

Medicina

Mouse Model of Middle Cerebral Artery Occlusion

Modello murino di Mezzo occlusione dell&#39;arteria cerebrale

Stroke is the most common fatal neurological disease in the United States 1. The majority of strokes (88%) result from blockage of blood vessels in the ...

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Bilaterale occlusione comune Arteria carotide come uno stimolo precondizionamento in grado di indurre tolleranza ischemica precoce di focale Ischemia Cerebrale

There is accumulating evidence, that ischemic preconditioning - a non-damaging ischemic challenge to the brain - confers a transient protection to a ...

Endothelin-1 Induced Middle Cerebral Artery Occlusion Model for Ischemic Stroke with Laser Doppler Flowmetry Guidance in Rat

L&#39;endotelina-1 indotta cerebrale media occlusione dell&#39;arteria modello per l&#39;ictus ischemico con guida laser flussimetria Doppler in Rat

Stroke is the number one cause of disability and third leading cause of death in the world, costing an estimated $70 billion in the United States in ...

Utilizing a Cranial Window to Visualize the Middle Cerebral Artery During Endothelin-1 Induced Middle Cerebral Artery Occlusion

Utilizzando una finestra craniale per visualizzare l&#39;arteria cerebrale media Durante endotelina-1 indotta da occlusione dell&#39;arteria cerebrale media

Creation of a cranial window is a method that allows direct visualization of structures on the cortical surface of the brain1-3. This technique can be ...

Optimized System for Cerebral Perfusion Monitoring in the Rat Stroke Model of Intraluminal Middle Cerebral Artery Occlusion

Sistema ottimizzato per il monitoraggio perfusione cerebrale nel modello Rat Stroke of intraluminale occlusione dell&#39;arteria cerebrale media

The translational potential of pre-clinical stroke research depends on the accuracy of experimental modeling. Cerebral perfusion monitoring in animal ...

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Corsa di modellazione in Mouse: Coagulazione permanente dell&#39;arteria cerebrale media distale

Stroke is the third most common cause of death and a main cause of acquired adult disability in developed countries. Only very limited therapeutical ...

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Embolic occlusione dell&#39;arteria cerebrale media (MCAO) per l&#39;ictus ischemico con omologhi coaguli di sangue nei ratti

Clinically, thrombolytic therapy with use of recombinant tissue plasminogen activator (tPA) remains the most effective treatment for acute ischemic ...

Quantification of Neurovascular Protection Following Repetitive Hypoxic Preconditioning and Transient Middle Cerebral Artery Occlusion in Mice

Quantificazione della Protezione Neurovascular seguito ripetitivo ipossico precondizionamento e transitoria occlusione dell&#39;arteria cerebrale media nei topi

Experimental animal models of stroke are invaluable tools for understanding stroke pathology and developing more effective treatment strategies. A 2 week ...

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Stroke typically occurs in elderly people with a range of comorbidities including carotid (or other arterial) atherosclerosis, high blood pressure, ...