Name: intraluminal middle cerebral artery occlusion (mcao) model for ischemic stroke with laser doppler flowmetry guidance in mice
Uploaded: 2011-05-08T14:00:00
Description: Watch this Scientific Journal Video about Intraluminal Middle Cerebral Artery Occlusion MCAO Model for Ischemic Stroke with Laser Doppler Flowmetry Guidance in Mice at JoVE.com

Este protocolo foi aprovado pelo Institutional Animal Care e Use Committee (IACUC) da Universidade da Flórida e está em conformidade com o &quot;Princípio do Laboratório Animal Care&quot; (NIH Publication No. 86-23, revisado em 1985). 1. Materiais <ol><li> Animais: Camundongos machos C57BL / 6 (Harlan Laboratories, Indianapolis, IN, EUA) pesando 20-26 gramas no momento da cirurgia. </li><li> Anestesia: <ol><li> Aparelho de anestesia: VetEquip sistema de anestesia inalatória (VetEquip ...

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	<li>. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Recommendations+on+stroke+prevention,+diagnosis,+and+therapy.+Report+of+the+WHO+Task+Force+on+Stroke+and+other+Cerebrovascular+Disorders.">Recommendations on stroke prevention, diagnosis, and therapy. Report of the WHO Task Force on Stroke and other Cerebrovascular Disorders.</a> Stroke; a journal of cerebral circulation. 20, 1407-1431 (1989).</li><li>Lloyd-Jones, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Heart+disease+and+stroke+statistics--2009+update:+a+report+from+the+American+Heart+Association+Statistics+Committee+and+Stroke+Statistics+Subcommittee.">Heart disease and stroke statistics--2009 update: a report from the American Heart Association Statistics Committee and Stroke Statistics Subcommittee.</a> Circulation. 119, e21-e181 (2009).</li><li>Longa, E. Z., Weinstein, P. R., Carlson, S., Cummins, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Reversible+middle+cerebral+artery+occlusion+without+craniectomy+in+rats.">Reversible middle cerebral artery occlusion without craniectomy in rats.</a> Stroke; a journal of cerebral circulation. 20, 84-91 (1989).</li><li>Koizumi, J., Yoshida, Y., Nakazawa, T., Ooneda, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Experimental+studies+of+ischemic+brain+edema.+I.+A+new+experimental+model+of+cerebral+embolism+in+rats+in+which+recirculation+can+be+introduced+in+the+ischemic+area.">Experimental studies of ischemic brain edema. I. A new experimental model of cerebral embolism in rats in which recirculation can be introduced in the ischemic area.</a> Jpn J Stroke. 8, 1-8 (1986).</li><li>Clark, W. M., Lessov, N. S., Dixon, M. P., Eckenstein, F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Monofilament+intraluminal+middle+cerebral+artery+occlusion+in+the+mouse.">Monofilament intraluminal middle cerebral artery occlusion in the mouse.</a> Neurol Res. 19, 641-648 (1997).</li><li>Fujii, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Strain-related+differences+in+susceptibility+to+transient+forebrain+ischemia+in+SV-129+and+C57black/6+mice.+.">Strain-related differences in susceptibility to transient forebrain ischemia in SV-129 and C57black/6 mice. .</a> Stroke; a journal of cerebral circulation. 28, 1805-1810 (1997).</li><li>Bederson, J. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Evaluation+of+2,3,5-triphenyltetrazolium+chloride+as+a+stain+for+detection+and+quantification+of+experimental+cerebral+infarction+in+rats.">Evaluation of 2,3,5-triphenyltetrazolium chloride as a stain for detection and quantification of experimental cerebral infarction in rats.</a> Stroke; a journal of cerebral circulation. 17, 1304-1308 (1986).</li><li>Qi, X., Hosoi, T., Okuma, Y., Kaneko, M., Nomura, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Sodium+4-phenylbutyrate+protects+against+cerebral+ischemic+injury.">Sodium 4-phenylbutyrate protects against cerebral ischemic injury.</a> Mol Pharmacol. 66, 899-908 (2004).</li><li>Barone, F. C., Knudsen, D. J., Nelson, A. H., Feuerstein, G. Z., Willette, R. N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mouse+strain+differences+in+susceptibility+to+cerebral+ischemia+are+related+to+cerebral+vascular+anatomy.">Mouse strain differences in susceptibility to cerebral ischemia are related to cerebral vascular anatomy.</a> J Cereb Blood Flow Metab. 13, 683-692 (1993).</li><li>Tureyen, K., Vemuganti, R., Sailor, K. A., Dempsey, R. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Ideal+suture+diameter+is+critical+for+consistent+middle+cerebral+artery+occlusion+in+mice.">Ideal suture diameter is critical for consistent middle cerebral artery occlusion in mice.</a> Neurosurgery. 56, 196-200 (2005).</li><li>Barber, P. A., Hoyte, L., Colbourne, F., Buchan, A. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Temperature-regulated+model+of+focal+ischemia+in+the+mouse:+a+study+with+histopathological+and+behavioral+outcomes.">Temperature-regulated model of focal ischemia in the mouse: a study with histopathological and behavioral outcomes.</a> Stroke; a journal of cerebral circulation. 35, 1720-1725 (2004).</li><li>Kapinya, K. J., Prass, K., Dirnagl, U. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Isoflurane+induced+prolonged+protection+against+cerebral+ischemia+in+mice:+a+redox+sensitive+mechanism?.">Isoflurane induced prolonged protection against cerebral ischemia in mice: a redox sensitive mechanism?.</a> Neuroreport. 13, 1431-1435 (2002).</li></ol>

O modelo MCAO é um modelo estabelecido que replica isquemia cerebral em espécies murina. Este modelo foi usado pela primeira vez em ratos e, posteriormente adaptado para ratos. Evitando craniectomia para eliminar a influência da manipulação cirúrgica na permeabilidade da barreira do sangue cerebral e pressão intracraniana é uma vantagem desta técnica. No entanto, muitas variáveis, tais como tensão diferenças relacionadas 10, tamanho da ponta de sutura 11, a duração da oclusão 6,...

<table border="1"><tbody><tr><th> Nome do reagente </th><th> Tipo </th><th> Companhia </th><th> Número de catálogo </th><th> Comentários (opcional) </th></tr><tr><td> Masculino camundongos C57BL / 6 </td><td> Animal </td><td> Harlan Laboratories, Indianapolis, IN, EUA </td><td></td><td> 20-26 grs </td></tr><tr><td> Microscópio de dissecção </td><td> Microscópio </td><td> Leica Microscópio estéreo MZ6 </td><td></td><td> Zoom 6.3:1 </td></tr><tr><td> Iluminador de fibra óptica </td><td> Iluminador </td><td> TechniQuip Corp, Livermore, CA, EUA </td><td></td><td> Fo1-150 </td></tr><tr><td> Isoflurano </td><td> Anestesia </td><td> Farmacêutica Baxter, Deerfield, IL </td><td> 1001936060 </td><td></td></tr><tr><td> Tesoura Iris SuperCut, em linha reta </td><td> Ferramenta cirúrgica </td><td> Precision mundo Instruments, Inc., Sarasota, FL, EUA </td><td> 14218 </td><td></td></tr><tr><td> Vannas micro-tesoura, em linha reta </td><td> Ferramenta cirúrgica </td><td> Precision mundo Instruments, Inc., Sarasota, FL, EUA </td><td> 14003 </td><td></td></tr><tr><td> Dumont multa forceps ponta </td><td> Ferramenta cirúrgica </td><td> Precision mundo Instruments, Inc., Sarasota, FL, EUA </td><td> 503290 </td><td></td></tr><tr><td> Clipe de navio </td><td> Ferramenta cirúrgica </td><td> Precision mundo Instruments, Inc., Sarasota, FL, EUA </td><td> 14120 </td><td></td></tr><tr><td> Exsicador High Frequency 900 </td><td> Ferramenta cirúrgica </td><td> Precision mundo Instruments, Inc., Sarasota, FL, EUA </td><td> 500397 </td><td></td></tr><tr><td> 7-0 sutura cirúrgica de monofilamento de nylon </td><td> Sutura </td><td> Ethicon, Inc., Somerville, NJ, EUA </td><td> 1647G </td><td> Sutura para oclusão </td></tr><tr><td> 7-0 sutura cirúrgica Silk </td><td> Sutura </td><td> Ethicon, Inc., Somerville, NJ, EUA </td><td> 7733G </td><td> Sutura para ligadura </td></tr><tr><td> Silicone RTV adesiva </td><td> Borracha de silicone </td><td> Precision mundo Instruments, Inc., Sarasota, FL, EUA </td><td> 1571 </td><td></td></tr><tr><td> Vetbond </td><td> Adesivo de tecido </td><td> 3M Company, St Paul, MN, EUA </td><td> 70200746587 </td><td></td></tr><tr><td> Puralube Pomada Vet </td><td> Pomada </td><td> Pharmaderm, Melville, NY, EUA </td><td></td><td></td></tr><tr><td> Buprenorfina hydrochlorode </td><td> Analgésicos </td><td> Hospira Inc., Lake Forest, Illinois, EUA </td><td> NDC 0409-2012-32 </td><td></td></tr><tr><td> Unidade Cauterização Térmica </td><td></td><td> Precision mundo Instruments, Inc., Sarasota, FL, EUA </td><td> 501292 </td><td></td></tr><tr><td> PeriFlux System 5000 </td><td> TCD fluxometria </td><td> Perimed, Estocolmo, Suécia </td><td></td><td></td></tr><tr><td> BAT-12 </td><td> Retal termômetro </td><td> Precision mundo Instruments, Inc., Sarasota, FL, EUA </td><td> BAT-12R </td><td></td></tr><tr><td> T / PUMP, TP600 </td><td> Cobertor térmico </td><td> Gaymar, NY, EUA </td><td></td><td></td></tr><tr><td> 2,3,5-trifeniltetrazólio cloreto </td><td> Reagente </td><td> Sigma-Aldrich Co., St Louis, MO, EUA </td><td> T8877 </td><td></td></tr><tr><td> Camundongos cérebro matriz slicer </td><td> Cérebro slicer </td><td> Zivic-Miller Lab., Inc., Allison Park, PA, EUA </td><td> BSMAS001-1 </td><td></td></tr></tbody></table>

intraluminal middle cerebral artery occlusion (mcao) model for ischemic stroke with laser doppler flowmetry guidance in mice

AVC é a terceira principal causa de morte e a principal causa de incapacidade no mundo, com um custo estimado de perto de US $ 70 bilhões nos Estados Unidos em 2009 1,2. O intraluminal cerebral média de oclusão da artéria modelo (MCAO) foi desenvolvido por Koizumi 4 em 1986 para simular esta patologia de grande impacto humano no rato. A modificação do método de MCAO mais tarde foi apresentado por Longa 3. Ambas as técnicas têm sido amplamente utilizados para identificar mecanismos moleculares de lesão cerebral resultante de acidente vascular cerebral isquêmico e potencial modalidades terapêuticas 5. Este método relativamente não-invasivo em ratos foi estendido para uso em camundongos para tirar proveito de transgênicos e knockout cepas 6,7. À isquemia focal modelo cerebral, uma sutura intraluminal é avançado através da artéria carótida interna para ocluir a base do MCA. Retração da sutura, após um período de tempo especificado imita reperfusão espontânea, mas a sutura pode também ser mantida em permanência. Este vídeo estará demonstrando as duas abordagens principais para a realização de procedimento MCAO intraluminal em ratos de forma gradual, bem como fornecer insights para potenciais inconvenientes e perigos. O tecido cerebral isquêmico, posteriormente, ser manchada por 2,3,5-trifeniltetrazólio cloreto (TTC) para avaliar a extensão do infarto cerebral 8.

Watch this Scientific Journal Video about Intraluminal Middle Cerebral Artery Occlusion MCAO Model for Ischemic Stroke with Laser Doppler Flowmetry Guidance in Mice at JoVE.com

Intraluminal Middle Cerebral Artery Occlusion MCAO Model for Ischemic Stroke with Laser Doppler Flowmetry Guidance in Mice

O intraluminal cerebral média de oclusão da artéria modelo (MCAO) é o modelo mais freqüente dentre os modelos experimentais acidente vascular cerebral isquêmico. Aqui vamos demonstrar todo o modelo em detalhes com o guia de Laser Doppler fluxometria, e os resultados do seu representante.

Intraluminal artéria cerebral média Oclusão Model (MCAO) para Acidente Vascular Cerebral Isquêmico com Laser Doppler Fluxometria Orientação em Ratos

Stroke is the third leading cause of death and the leading cause of disability in the world, with an estimated cost of near $70 billion 
in the United ...

Research

JoVE Journal

Medicine

Intraluminal Middle Cerebral Artery Occlusion (MCAO) Model for Ischemic Stroke with Laser Doppler Flowmetry Guidance in Mice

Modeling Stroke in Mice - Middle Cerebral Artery Occlusion with the Filament Model

Curso de modelagem em Ratos - Oclusão da Artéria Cerebral Média com o modelo de Filamento

Stroke is among the most frequent causes of death and adult disability, especially in highly developed countries. However, treatment options to date are ...

Medicina

Mouse Model of Middle Cerebral Artery Occlusion

Modelo do rato de Oclusão da Artéria Cerebral Média

Stroke is the most common fatal neurological disease in the United States 1. The majority of strokes (88%) result from blockage of blood vessels in the ...

Bilateral Common Carotid Artery Occlusion as an Adequate Preconditioning Stimulus to Induce Early Ischemic Tolerance to Focal Cerebral Ischemia

Oclusão bilateral artéria carótida comum como um estímulo pré-condicionamento adequados para induzir a tolerância isquêmica início da Isquemia Cerebral Focal

There is accumulating evidence, that ischemic preconditioning - a non-damaging ischemic challenge to the brain - confers a transient protection to a ...

Endothelin-1 Induced Middle Cerebral Artery Occlusion Model for Ischemic Stroke with Laser Doppler Flowmetry Guidance in Rat

Endotelina-1 Induzida Médio modelo de oclusão da artéria cerebral para AVC isquêmico com Laser Orientação Fluxometria Doppler em ratos

Stroke is the number one cause of disability and third leading cause of death in the world, costing an estimated $70 billion in the United States in ...

Utilizing a Cranial Window to Visualize the Middle Cerebral Artery During Endothelin-1 Induced Middle Cerebral Artery Occlusion

Utilizando uma janela craniana para Visualizar a artéria cerebral média Durante endotelina 1 Induzida por oclusão da artéria cerebral média

Creation of a cranial window is a method that allows direct visualization of structures on the cortical surface of the brain1-3. This technique can be ...

Optimized System for Cerebral Perfusion Monitoring in the Rat Stroke Model of Intraluminal Middle Cerebral Artery Occlusion

Sistema otimizado para Monitorização de perfusão cerebral no modelo do rato do curso de oclusão da artéria cerebral média intraluminal

The translational potential of pre-clinical stroke research depends on the accuracy of experimental modeling. Cerebral perfusion monitoring in animal ...

Modeling Stroke in Mice: Permanent Coagulation of the Distal Middle Cerebral Artery

Curso de modelagem em Ratos: Coagulação permanente da artéria distal Cerebral Média

Stroke is the third most common cause of death and a main cause of acquired adult disability in developed countries. Only very limited therapeutical ...

Embolic Middle Cerebral Artery Occlusion (MCAO) for Ischemic Stroke with Homologous Blood Clots in Rats

Embolic Middle Cerebral Artery Occlusion MCAO for Ischemic Stroke with Homologous Blood Clots in Rats

Embolic artéria cerebral média Oclusão (MCAO) para Acidente Vascular Cerebral Isquêmico com homólogos Coágulos de sangue em ratos

Clinically, thrombolytic therapy with use of recombinant tissue plasminogen activator (tPA) remains the most effective treatment for acute ischemic ...

Quantification of Neurovascular Protection Following Repetitive Hypoxic Preconditioning and Transient Middle Cerebral Artery Occlusion in Mice

Quantificação de Proteção Neurovascular sequência repetitiva Hypoxic Pré-condicionamento e Transient Oriente oclusão da artéria cerebral em ratos

Experimental animal models of stroke are invaluable tools for understanding stroke pathology and developing more effective treatment strategies. A 2 week ...

Performing Permanent Distal Middle Cerebral with Common Carotid Artery Occlusion in Aged Rats to Study Cortical Ischemia with Sustained Disability

Realizando Permanente Distal Oriente cerebral com carótida comum Oclusão da Artéria em ratos idosos para estudar Cortical Isquemia com Deficiência Sustentada

Stroke typically occurs in elderly people with a range of comorbidities including carotid (or other arterial) atherosclerosis, high blood pressure, ...