Name: intraluminal middle cerebral artery occlusion (mcao) model for ischemic stroke with laser doppler flowmetry guidance in mice
Uploaded: 2011-05-08T14:00:00
Description: Watch this Scientific Journal Video about Intraluminal Middle Cerebral Artery Occlusion MCAO Model for Ischemic Stroke with Laser Doppler Flowmetry Guidance in Mice at JoVE.com

Detta protokoll godkändes av Institutional Animal Care och användning kommittén (IACUC) vid University of Florida och är i överensstämmelse med &quot;principen om Laboratory Animal Care&quot; (NIH publikation nr 86-23, reviderad 1985). 1. Material <ol><li> Djur: Man C57BL / 6 möss (Harlan Laboratories, Indianapolis, IN, USA) som väger 20-26 gram vid tidpunkten för operation. </li><li> Anestesi: <ol><li> Anestesiapparaten: VetEquip inhalationsanestesi systemet (VetEquip Inc., San F...

<ol>
	<li>. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Recommendations+on+stroke+prevention,+diagnosis,+and+therapy.+Report+of+the+WHO+Task+Force+on+Stroke+and+other+Cerebrovascular+Disorders.">Recommendations on stroke prevention, diagnosis, and therapy. Report of the WHO Task Force on Stroke and other Cerebrovascular Disorders.</a> Stroke; a journal of cerebral circulation. 20, 1407-1431 (1989).</li><li>Lloyd-Jones, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Heart+disease+and+stroke+statistics--2009+update:+a+report+from+the+American+Heart+Association+Statistics+Committee+and+Stroke+Statistics+Subcommittee.">Heart disease and stroke statistics--2009 update: a report from the American Heart Association Statistics Committee and Stroke Statistics Subcommittee.</a> Circulation. 119, e21-e181 (2009).</li><li>Longa, E. Z., Weinstein, P. R., Carlson, S., Cummins, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Reversible+middle+cerebral+artery+occlusion+without+craniectomy+in+rats.">Reversible middle cerebral artery occlusion without craniectomy in rats.</a> Stroke; a journal of cerebral circulation. 20, 84-91 (1989).</li><li>Koizumi, J., Yoshida, Y., Nakazawa, T., Ooneda, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Experimental+studies+of+ischemic+brain+edema.+I.+A+new+experimental+model+of+cerebral+embolism+in+rats+in+which+recirculation+can+be+introduced+in+the+ischemic+area.">Experimental studies of ischemic brain edema. I. A new experimental model of cerebral embolism in rats in which recirculation can be introduced in the ischemic area.</a> Jpn J Stroke. 8, 1-8 (1986).</li><li>Clark, W. M., Lessov, N. S., Dixon, M. P., Eckenstein, F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Monofilament+intraluminal+middle+cerebral+artery+occlusion+in+the+mouse.">Monofilament intraluminal middle cerebral artery occlusion in the mouse.</a> Neurol Res. 19, 641-648 (1997).</li><li>Fujii, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Strain-related+differences+in+susceptibility+to+transient+forebrain+ischemia+in+SV-129+and+C57black/6+mice.+.">Strain-related differences in susceptibility to transient forebrain ischemia in SV-129 and C57black/6 mice. .</a> Stroke; a journal of cerebral circulation. 28, 1805-1810 (1997).</li><li>Bederson, J. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Evaluation+of+2,3,5-triphenyltetrazolium+chloride+as+a+stain+for+detection+and+quantification+of+experimental+cerebral+infarction+in+rats.">Evaluation of 2,3,5-triphenyltetrazolium chloride as a stain for detection and quantification of experimental cerebral infarction in rats.</a> Stroke; a journal of cerebral circulation. 17, 1304-1308 (1986).</li><li>Qi, X., Hosoi, T., Okuma, Y., Kaneko, M., Nomura, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Sodium+4-phenylbutyrate+protects+against+cerebral+ischemic+injury.">Sodium 4-phenylbutyrate protects against cerebral ischemic injury.</a> Mol Pharmacol. 66, 899-908 (2004).</li><li>Barone, F. C., Knudsen, D. J., Nelson, A. H., Feuerstein, G. Z., Willette, R. N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mouse+strain+differences+in+susceptibility+to+cerebral+ischemia+are+related+to+cerebral+vascular+anatomy.">Mouse strain differences in susceptibility to cerebral ischemia are related to cerebral vascular anatomy.</a> J Cereb Blood Flow Metab. 13, 683-692 (1993).</li><li>Tureyen, K., Vemuganti, R., Sailor, K. A., Dempsey, R. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Ideal+suture+diameter+is+critical+for+consistent+middle+cerebral+artery+occlusion+in+mice.">Ideal suture diameter is critical for consistent middle cerebral artery occlusion in mice.</a> Neurosurgery. 56, 196-200 (2005).</li><li>Barber, P. A., Hoyte, L., Colbourne, F., Buchan, A. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Temperature-regulated+model+of+focal+ischemia+in+the+mouse:+a+study+with+histopathological+and+behavioral+outcomes.">Temperature-regulated model of focal ischemia in the mouse: a study with histopathological and behavioral outcomes.</a> Stroke; a journal of cerebral circulation. 35, 1720-1725 (2004).</li><li>Kapinya, K. J., Prass, K., Dirnagl, U. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Isoflurane+induced+prolonged+protection+against+cerebral+ischemia+in+mice:+a+redox+sensitive+mechanism?.">Isoflurane induced prolonged protection against cerebral ischemia in mice: a redox sensitive mechanism?.</a> Neuroreport. 13, 1431-1435 (2002).</li></ol>

Den kortex modellen är en etablerad modell som replikerar cerebral ischemi i murina arter. Denna modell användes först på råttor och därefter anpassas till möss. Undvika craniectomy att eliminera inverkan av kirurgisk manipulation av blod-hjärnbarriären permeabilitet och intrakraniellt tryck är en fördel med denna teknik. Däremot kan många variabler, såsom stam-relaterade skillnader 10, storlek sutur spets 11, längd ocklusion 6, kroppstemperatur 12, anestesi <su...

<table border="1"><tbody><tr><th> Reagensets namn </th><th> Typ </th><th> Företag </th><th> Katalognummer </th><th> Kommentarer (frivilligt) </th></tr><tr><td> Man C57BL / 6 möss </td><td> Animal </td><td> Harlan Laboratories, Indianapolis, IN, USA </td><td></td><td> 20-26 GRS </td></tr><tr><td> Dissection mikroskop </td><td> Mikroskop </td><td> Leica stereomikroskop MZ6 </td><td></td><td> 6.3:1 zoom </td></tr><tr><td> Fiberoptisk belysning </td><td> Illuminator </td><td> TechniQuip Corp, Livermore, CA, USA </td><td></td><td> FO1-150 </td></tr><tr><td> Isofluran </td><td> Anestesi </td><td> Baxter Pharmaceutics, Deerfield, IL </td><td> 1001936060 </td><td></td></tr><tr><td> SuperCut Iris sax, rak </td><td> Kirurgiska verktyg </td><td> Världen Precision Instruments, Inc., Sarasota, FL, USA </td><td> 14.218 </td><td></td></tr><tr><td> Vannas mikro-sax, rak </td><td> Kirurgiska verktyg </td><td> Världen Precision Instruments, Inc., Sarasota, FL, USA </td><td> 14.003 </td><td></td></tr><tr><td> Dumont fin spets tång </td><td> Kirurgiska verktyg </td><td> Världen Precision Instruments, Inc., Sarasota, FL, USA </td><td> 503.290 </td><td></td></tr><tr><td> Fartyg klipp </td><td> Kirurgiska verktyg </td><td> Världen Precision Instruments, Inc., Sarasota, FL, USA </td><td> 14.120 </td><td></td></tr><tr><td> High Frequency Exsickator 900 </td><td> Kirurgiska verktyg </td><td> Världen Precision Instruments, Inc., Sarasota, FL, USA </td><td> 500.397 </td><td></td></tr><tr><td> 7-0 kirurgiska nylon monofilament sutur </td><td> Sutur </td><td> Ethicon, Inc., Somerville, NJ, USA </td><td> 1647G </td><td> Sutur för ocklusion </td></tr><tr><td> 7-0 kirurgiska Silk sutur </td><td> Sutur </td><td> Ethicon, Inc., Somerville, NJ, USA </td><td> 7733G </td><td> Sutur för ligering </td></tr><tr><td> Silikon eltejp </td><td> Silikongummi </td><td> Världen Precision Instruments, Inc., Sarasota, FL, USA </td><td> 1571 </td><td></td></tr><tr><td> Vetbond </td><td> Tissue lim </td><td> 3M Company, St Paul, MN, USA </td><td> 70200746587 </td><td></td></tr><tr><td> Puralube Vet Salva </td><td> Ögonsalva </td><td> Pharmaderm, Melville, NY, USA </td><td></td><td></td></tr><tr><td> Buprenorfin hydrochlorode </td><td> Analgetika </td><td> Hospira Inc., Lake Forest, IL, USA </td><td> NDC 0409-2012-32 </td><td></td></tr><tr><td> Termisk cautery Enhet </td><td></td><td> Världen Precision Instruments, Inc., Sarasota, FL, USA </td><td> 501.292 </td><td></td></tr><tr><td> PeriFlux System 5000 </td><td> TCD flowmetry </td><td> Perimed, Stockholm, Sweeden </td><td></td><td></td></tr><tr><td> BAT-12 </td><td> Rektal termometer </td><td> Världen Precision Instruments, Inc., Sarasota, FL, USA </td><td> BAT-12R </td><td></td></tr><tr><td> T / pump, TP600 </td><td> Värmefilt </td><td> Gaymar, NY, USA </td><td></td><td></td></tr><tr><td> 2,3,5-triphenyltetrazolium klorid </td><td> Reagens </td><td> Sigma-Aldrich Co, St Louis, MO, USA </td><td> T8877 </td><td></td></tr><tr><td> Möss hjärnan matris slicer </td><td> Brain skivare </td><td> Zivic-Miller Lab. Inc., Allison Park, PA, USA </td><td> BSMAS001-1 </td><td></td></tr></tbody></table>

intraluminal middle cerebral artery occlusion (mcao) model for ischemic stroke with laser doppler flowmetry guidance in mice

Stroke är den tredje vanligaste dödsorsaken och den vanligaste orsaken till funktionshinder i världen, med en beräknad kostnad på nära $ 70 miljarder USA under 2009 1,2. Den intraluminal mellersta cerebral artär ocklusion (kortex) modellen utvecklades av Koizumi 4 1986 för att simulera detta effektfulla mänskliga patologi hos råtta. En ändring av kortex metoden senare presenterades av Longa 3. Båda teknikerna har ofta använts för att identifiera molekylära mekanismer för hjärnskador till följd av ischemisk stroke och potentiella terapeutiska metoder 5. Denna relativt icke-invasiv metod hos råttor har utvidgats till att använda i möss att dra nytta av transgena och stammar knockout 6,7. Att modellera fokal cerebral ischemi, är en intraluminal sutur avancerat via det interna halspulsådern för att täppa till basen på MCA. Dra tillbaka sutur efter en viss tid härmar spontan reperfusion, men sutur kan också vara permanent behållas. Denna video kommer att demonstrera de två stora metoder för att utföra intraluminal kortex förfarande i möss i en stegvis, samt att ge insikter för potentiella nackdelar och fallgropar. Den ischemisk hjärnvävnad kommer senare att färgas av 2,3,5-triphenyltetrazolium klorid (TTC) för att utvärdera omfattningen av hjärninfarkt 8.

Watch this Scientific Journal Video about Intraluminal Middle Cerebral Artery Occlusion MCAO Model for Ischemic Stroke with Laser Doppler Flowmetry Guidance in Mice at JoVE.com

Intraluminal Middle Cerebral Artery Occlusion MCAO Model for Ischemic Stroke with Laser Doppler Flowmetry Guidance in Mice

Den intraluminal mellersta cerebral artär ocklusion (kortex) modellen är den vanligaste använda modellen bland experimentella ischemisk stroke modeller. Här kommer vi att visa hela modellen i detalj med guide med Laser Doppler flowmetry, och dess resultat representant.

Intraluminal Middle Cerebral artärocklusion (kortex) Modell för ischemisk stroke med Laser Doppler Flowmetry Vägledning hos möss

Stroke is the third leading cause of death and the leading cause of disability in the world, with an estimated cost of near $70 billion 
in the United ...

Research

JoVE Journal

Medicine

Intraluminal Middle Cerebral Artery Occlusion (MCAO) Model for Ischemic Stroke with Laser Doppler Flowmetry Guidance in Mice

Modeling Stroke in Mice - Middle Cerebral Artery Occlusion with the Filament Model

Modellering stroke hos möss - Middle Cerebral artärocklusion med glödlamporna modell

Stroke is among the most frequent causes of death and adult disability, especially in highly developed countries. However, treatment options to date are ...

Mouse Model of Middle Cerebral Artery Occlusion

Musmodell av Middle Cerebral artärocklusion

Stroke is the most common fatal neurological disease in the United States 1. The majority of strokes (88%) result from blockage of blood vessels in the ...

Bilateral Common Carotid Artery Occlusion as an Adequate Preconditioning Stimulus to Induce Early Ischemic Tolerance to Focal Cerebral Ischemia

Bilateralt gemensamma halspulsådern Ocklusion som en fullgod Konditioneringscykel Stimulus att framkalla tidig ischemisk tolerans till Focal cerebral ischemi

There is accumulating evidence, that ischemic preconditioning - a non-damaging ischemic challenge to the brain - confers a transient protection to a ...

Endothelin-1 Induced Middle Cerebral Artery Occlusion Model for Ischemic Stroke with Laser Doppler Flowmetry Guidance in Rat

Endotelin-1 inducerad Middle cerebral artärocklusion modell för ischemisk stroke med laser Doppler Flowmetry Vägledning i råtta

Stroke is the number one cause of disability and third leading cause of death in the world, costing an estimated $70 billion in the United States in ...

Utilizing a Cranial Window to Visualize the Middle Cerebral Artery During Endothelin-1 Induced Middle Cerebral Artery Occlusion

Använda en Kranial fönster för att visualisera den mellersta cerebrala artären under Endotelin-1 inducerad Middle cerebral artärocklusion

Creation of a cranial window is a method that allows direct visualization of structures on the cortical surface of the brain1-3. This technique can be ...

Optimized System for Cerebral Perfusion Monitoring in the Rat Stroke Model of Intraluminal Middle Cerebral Artery Occlusion

Optimerat system för cerebral perfusion övervakning i råtta Stroke Modell av Intraluminal Middle cerebral artärocklusion

The translational potential of pre-clinical stroke research depends on the accuracy of experimental modeling. Cerebral perfusion monitoring in animal ...

Modeling Stroke in Mice: Permanent Coagulation of the Distal Middle Cerebral Artery

Modellering stroke hos möss: Permanent Koagulation av den distala Middle Cerebral Artery

Stroke is the third most common cause of death and a main cause of acquired adult disability in developed countries. Only very limited therapeutical ...

Embolic Middle Cerebral Artery Occlusion (MCAO) for Ischemic Stroke with Homologous Blood Clots in Rats

Embolic Middle Cerebral Artery Occlusion MCAO for Ischemic Stroke with Homologous Blood Clots in Rats

Emboli Middle Cerebral artärocklusion (kortex) för ischemisk stroke med Homologa Blodproppar i råttor

Clinically, thrombolytic therapy with use of recombinant tissue plasminogen activator (tPA) remains the most effective treatment for acute ischemic ...

Quantification of Neurovascular Protection Following Repetitive Hypoxic Preconditioning and Transient Middle Cerebral Artery Occlusion in Mice

Kvantifiering av neurovaskulär skydd Efter Repetitiva Hypoxisk Förkonditionering och Transient Middle Cerebral artärocklusion hos möss

Experimental animal models of stroke are invaluable tools for understanding stroke pathology and developing more effective treatment strategies. A 2 week ...

Performing Permanent Distal Middle Cerebral with Common Carotid Artery Occlusion in Aged Rats to Study Cortical Ischemia with Sustained Disability

Utföra ständiga Distal Middle Cerebral med gemensamma hals artärocklusion i åldrade råttor för att studera kortikal ischemi med fördröjd funktionshinder

Stroke typically occurs in elderly people with a range of comorbidities including carotid (or other arterial) atherosclerosis, high blood pressure, ...