Name: intraluminal middle cerebral artery occlusion (mcao) model for ischemic stroke with laser doppler flowmetry guidance in mice
Uploaded: 2011-05-08T14:00:00
Description: Watch this Scientific Journal Video about Intraluminal Middle Cerebral Artery Occlusion MCAO Model for Ischemic Stroke with Laser Doppler Flowmetry Guidance in Mice at JoVE.com

Bu protokol, Kurumsal Hayvan Bakım tarafından onaylanmış ve Florida Üniversitesi&#39;nde Komitesi (IACUC) kullanın ve &quot;Laboratuvar Hayvanları Bakım ilkesi&quot; (NIH yayın No 86-23, 1985 revize) ile uyum içerisindedir. 1. Malzemeler <ol><li> Hayvanlar: cerrahi anda 20-26 gram ağırlığında Erkek C57BL / 6 fareler (Harlan Laboratuvarları, Indianapolis, IN, ABD). </li><li> Anestezi: <ol><li> Anestezi makinesi: VetEquip inhalasyon anestezi sistemi (VetEquip A.Ş., Pleasanto...

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MCAO model fare türlerinde serebral iskemi çoğaltır kurulmuş bir model. Bu model ilk sıçanlarda kullanılır ve bundan sonra farelere adapte oldu. Kraniektomi kaçınmak, kan-beyin bariyeri geçirgenliği ve intrakranial basınç cerrahi manipülasyonu etkisini ortadan kaldırmak için bu tekniğin bir avantaj. Ancak, ilgili gerilim farklılıkları 10, 6, oklüzyon vücut ısısı 12, anestezi 13 sütür ucu 11, süresi büyüklüğü ve diğer faktörler gibi pek çok...

<table border="1"><tbody><tr><th> Reaktifin Adı </th><th> Tip </th><th> Şirket </th><th> Katalog numarası </th><th> Yorumlar (isteğe bağlı) </th></tr><tr><td> Erkek C57BL / 6 fareler </td><td> Hayvan </td><td> Harlan Laboratuvarları, Indianapolis, IN, ABD </td><td></td><td> 20-26 gr </td></tr><tr><td> Diseksiyon mikroskobu </td><td> Mikroskop </td><td> Leica Stereo Mikroskop MZ6 </td><td></td><td> 6.3:1 zum </td></tr><tr><td> Fiber optik aydınlatma </td><td> Aydınlatıcı </td><td> TechniQuip Corp, Livermore, CA, USA </td><td></td><td> FO1-150 </td></tr><tr><td> İsofluranın </td><td> Anestezi </td><td> Baxter İlaç, Deerfield, IL </td><td> 1001936060 </td><td></td></tr><tr><td> Iris makas düz SUPERCUT </td><td> Cerrahi araç </td><td> Dünya Precision Instruments, Inc, Sarasota, FL, ABD </td><td> 14218 </td><td></td></tr><tr><td> Vannas mikro-makas, düz </td><td> Cerrahi araç </td><td> Dünya Precision Instruments, Inc, Sarasota, FL, ABD </td><td> 14003 </td><td></td></tr><tr><td> Dumont ince ucu forseps </td><td> Cerrahi araç </td><td> Dünya Precision Instruments, Inc, Sarasota, FL, ABD </td><td> 503290 </td><td></td></tr><tr><td> Gemi klip </td><td> Cerrahi araç </td><td> Dünya Precision Instruments, Inc, Sarasota, FL, ABD </td><td> 14120 </td><td></td></tr><tr><td> Yüksek Frekans Desikatör 900 </td><td> Cerrahi araç </td><td> Dünya Precision Instruments, Inc, Sarasota, FL, ABD </td><td> 500397 </td><td></td></tr><tr><td> 7-0 cerrahi naylon monofilament sütür </td><td> Dikiş </td><td> Ethicon, Inc Somerville, NJ, ABD </td><td> 1647G </td><td> Oklüzyonu Sütür </td></tr><tr><td> 7-0 cerrahi İpek sütür </td><td> Dikiş </td><td> Ethicon, Inc Somerville, NJ, ABD </td><td> 7733G </td><td> Ligasyonu için sütür </td></tr><tr><td> Silikon RTV yapıştırıcı </td><td> Silikon kauçuk </td><td> Dünya Precision Instruments, Inc, Sarasota, FL, ABD </td><td> 1571 </td><td></td></tr><tr><td> Vetbond </td><td> Doku yapıştırıcısı </td><td> 3M Company, St Paul, MN, ABD </td><td> 70200746587 </td><td></td></tr><tr><td> Puralube Veteriner Merhem </td><td> Göz merhemi </td><td> Pharmaderm, Melville, NY, ABD </td><td></td><td></td></tr><tr><td> Buprenorfin hydrochlorode </td><td> Analjezikler </td><td> Hospira A.Ş., Lake Forest, IL, ABD </td><td> NDC 0409-2012-32 </td><td></td></tr><tr><td> Termal Koter Ünitesi </td><td></td><td> Dünya Precision Instruments, Inc, Sarasota, FL, ABD </td><td> 501292 </td><td></td></tr><tr><td> PeriFlux Sistemi 5000 </td><td> TCD flowmetre </td><td> Perimed, Stockholm, İsveç </td><td></td><td></td></tr><tr><td> BAT-12 </td><td> Rektal termometre </td><td> Dünya Precision Instruments, Inc, Sarasota, FL, ABD </td><td> Bat-12R </td><td></td></tr><tr><td> T / POMPA, TP600 </td><td> Termal battaniye </td><td> Gaymar, NY, ABD </td><td></td><td></td></tr><tr><td> 2,3,5-triphenyltetrazolium chloride </td><td> Reaktif </td><td> Sigma-Aldrich Co, St Louis, MO, USA </td><td> T8877 </td><td></td></tr><tr><td> Fareler beyin matris dilimleme makinesi </td><td> Beyin dilimleme makinesi </td><td> Zivic-Miller Lab. Inc, Allison Park, PA, USA </td><td> BSMAS001-1 </td><td></td></tr></tbody></table>

intraluminal middle cerebral artery occlusion (mcao) model for ischemic stroke with laser doppler flowmetry guidance in mice

İnme, 1,2 2009 yılında Amerika Birleşik Devletleri 70 milyar ABD doları yakın bir tahmini maliyeti, üçüncü önde gelen ölüm nedeni ve özürlülük dünyanın önde gelen nedenidir. Koizumi 4 sıçan bu etkileyici insan patoloji simüle etmek için 1986 yılında intraluminal orta serebral arter tıkanıklığı (MCAO) modeli geliştirilmiştir. MCAO yöntemi bir değişiklik daha sonra Longa 3 tarafından takdim edildi. Her iki teknikte de yaygın iskemik inme ve olası tedavi yöntemleri 5 kaynaklanan beyin hasarının moleküler mekanizmaları tanımlamak için kullanılmaktadır. Bu nispeten noninvaziv bir metod sıçanlarda, farelerde transgenik ve nakavt suşları 6,7 yararlanmak kullanmak uzatıldı . Fokal serebral iskemi modeli için, intraluminal sütür internal karotid arter yoluyla MCA tabanı kapamak için ileri düzeydedir. Katlanması zaman belirli bir süre sonra dikiş spontan reperfüzyon taklit eder, ancak sütür de kalıcı olarak muhafaza edilebilir. Bu video, kademeli bir biçimde farelerde intraluminal MCAO prosedürü gerçekleştirmek için iki ana yaklaşım gösteren yanı sıra potansiyel sakıncaları ve tuzaklar anlayışlar olacaktır. Iskemik beyin dokusu daha sonra beyin enfarktüsü 8 ölçüde değerlendirmek için 2,3,5-triphenyltetrazolium klorür (TTC) ile boyanan olacaktır .

Watch this Scientific Journal Video about Intraluminal Middle Cerebral Artery Occlusion MCAO Model for Ischemic Stroke with Laser Doppler Flowmetry Guidance in Mice at JoVE.com

Intraluminal Middle Cerebral Artery Occlusion MCAO Model for Ischemic Stroke with Laser Doppler Flowmetry Guidance in Mice

Intraluminal orta serebral arter tıkanıklığı (MCAO) modeli deneysel iskemik inme modelleri arasında en sık kullanılan model. Burada Lazer Doppler flowmetre kılavuzu ile ayrıntılı olarak tüm model ve onun temsilcisi sonuçları gösterecektir.

Fare lazer Doppler flowmetre Rehberlik İskemik İnme intraluminal Orta Serebral Arter Tıkanıklığı (MCAO) Model

Stroke is the third leading cause of death and the leading cause of disability in the world, with an estimated cost of near $70 billion 
in the United ...

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Medicine

Intraluminal Middle Cerebral Artery Occlusion (MCAO) Model for Ischemic Stroke with Laser Doppler Flowmetry Guidance in Mice

Modeling Stroke in Mice - Middle Cerebral Artery Occlusion with the Filament Model

Fare Modelleme İnme - Filament Model Orta Serebral Arter Tıkanıklığı

Stroke is among the most frequent causes of death and adult disability, especially in highly developed countries. However, treatment options to date are ...

Mouse Model of Middle Cerebral Artery Occlusion

Orta Serebral Arter Tıkanıklığı Fare Modeli

Stroke is the most common fatal neurological disease in the United States 1. The majority of strokes (88%) result from blockage of blood vessels in the ...

Bilateral Common Carotid Artery Occlusion as an Adequate Preconditioning Stimulus to Induce Early Ischemic Tolerance to Focal Cerebral Ischemia

Odak Serebral İskemi Erken İskemik Tolerans tetikleyebilecek bir Yeterli Önkoşullama Uyarıcı olarak bilateral Ortak Karotis Arter Tıkanıklığı

There is accumulating evidence, that ischemic preconditioning - a non-damaging ischemic challenge to the brain - confers a transient protection to a ...

Endothelin-1 Induced Middle Cerebral Artery Occlusion Model for Ischemic Stroke with Laser Doppler Flowmetry Guidance in Rat

Rat Lazer Doppler Flowmetri Rehberlik ile İskemik İnme için Endotelin-1 Bağlı Orta Serebral Arter Tıkanıklığı Modeli

Stroke is the number one cause of disability and third leading cause of death in the world, costing an estimated $70 billion in the United States in ...

Utilizing a Cranial Window to Visualize the Middle Cerebral Artery During Endothelin-1 Induced Middle Cerebral Artery Occlusion

Endotelin-1 Bağlı Orta Serebral Arter Tıkanıklığı sırasında Orta Serebral Arter görselleştirin bir Kranial Pencere kullanmak

Creation of a cranial window is a method that allows direct visualization of structures on the cortical surface of the brain1-3. This technique can be ...

Optimized System for Cerebral Perfusion Monitoring in the Rat Stroke Model of Intraluminal Middle Cerebral Artery Occlusion

Intraluminar Orta Serebral Arter Tıkanıklığı ve Sıçan Strok Model Serebral Perfüzyon İzleme için Optimize Sistemi

The translational potential of pre-clinical stroke research depends on the accuracy of experimental modeling. Cerebral perfusion monitoring in animal ...

Modeling Stroke in Mice: Permanent Coagulation of the Distal Middle Cerebral Artery

Farelerde Modelleme İnme: Distal Orta Serebral Arter Daimi Pıhtılaşma

Stroke is the third most common cause of death and a main cause of acquired adult disability in developed countries. Only very limited therapeutical ...

Embolic Middle Cerebral Artery Occlusion (MCAO) for Ischemic Stroke with Homologous Blood Clots in Rats

Embolic Middle Cerebral Artery Occlusion MCAO for Ischemic Stroke with Homologous Blood Clots in Rats

Sıçanlar Homolog Kan pıhtısı ile İskemik İnme emboli Orta Serebral Arter Tıkanıklığı (MCAO)

Clinically, thrombolytic therapy with use of recombinant tissue plasminogen activator (tPA) remains the most effective treatment for acute ischemic ...

Quantification of Neurovascular Protection Following Repetitive Hypoxic Preconditioning and Transient Middle Cerebral Artery Occlusion in Mice

Farelerde Tekrarlanan Hipoksik Önkoşullanma ve Geçici Orta Serebral Arter Tıkanıklığı ardından Nörovasküler Koruma Kantitasyonu

Experimental animal models of stroke are invaluable tools for understanding stroke pathology and developing more effective treatment strategies. A 2 week ...

Performing Permanent Distal Middle Cerebral with Common Carotid Artery Occlusion in Aged Rats to Study Cortical Ischemia with Sustained Disability

Yaşlı Sıçanlarda Ortak Karotis Arter Tıkanıklığı ile Kalıcı Distal Orta Serebral Sahne Kortikal İskemi Sürekli Engelli Eğitim için

Stroke typically occurs in elderly people with a range of comorbidities including carotid (or other arterial) atherosclerosis, high blood pressure, ...