Name: shape memory polymers for active cell culture
Uploaded: 2011-07-04T15:00:00
Description: Watch this Scientific Journal Video about Shape Memory Polymers for Active Cell Culture at JoVE.com

作者要感谢提供技术援助与ACC基材的准备凯利A.伯克。的文章发表在生物材料，戴维斯KA， 等 ，动态细胞行为的形状记忆聚合物衬底材料 ，DOI：10.1016/j.biomaterials.2010.12.006，版权爱思唯尔（2011年）的基础上。基于这种材料是由美国国家科学基金会的支持下批准号DMR - 0907578工作。

1。温紫外光固化的NOA63 <ol><li>开发一个自定义的固化室商会使用载玻片（75毫米x 25毫米x 1毫米），1毫米厚的聚四氟乙烯垫片，及铝合金板（75毫米× 25毫米× 3毫米），如图1所示。使用小型长尾夹一起举行。 </li><li>通过在聚四氟乙烯垫片孔使用18号针头注入到腔NOA63。 NOA63可以轻轻加热，以减轻注射。 </li><li>广场上设置在125 ° C，并允许以一个统一的温度加热5分钟热板腔。 </li><li>预...

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的NOA63 的 Tg可以很容易地通过固化温度控制。我们用这个生成SMP基板，可以在单元格中的兼容范围引发。 NOA63是由水，降低干燥的 Tg塑化，所以我们增加了固化在125 ° C至30日和37 ° C之间移动的Tg范围湿干的 Tg 激活的细胞培养基质表明，能够控制细胞的行为。微丝重组的结果，强调控制和化验动态基板上的细胞行为的潜力。硫化模具和压花形状的基础?...

<table border="1"><tbody><tr><td>试剂或仪器名称</td><td>公司</td><td>目录编号</td><td>评论（可选） </td></tr><tr><td> NOA63 </td><td>诺兰产品公司</td><td> NOA63 </td><td>批号111 </td></tr><tr><td>狗骨头打孔</td><td> TestResource公司Shakopee监狱，MN </td><td></td><td>按比例缩小的IV型狗骨头（ASTM D638 - 03） </td></tr><tr><td>台式液压出版社</td><td>卡弗</td><td> 3851 </td><td></td></tr><tr><td> C3H10T1 / 2小鼠胚胎成纤维</td><td> ATCC </td><td> CCL - 226 </td><td></td></tr><tr><td>生物安全柜</td><td>赛默飞世尔</td><td> 1357 </td><td></td></tr><tr><td>紫外灯</td><td> Spectroline </td><td> SB - 100PC </td><td></td></tr><tr><td>动态力学分析仪（DMA） </td><td> TA仪器公司</td><td> Q800 </td><td></td></tr><tr><td>倒置荧光显微镜</td><td>徕卡</td><td>徕卡的DMI 4000B </td><td></td></tr><tr><td>激光共聚焦显微镜</td><td>蔡司</td><td> LSM 710 </td><td> 20x/0.8 NA空气或一个40x/1.30无油客观</td></tr></tbody></table>

shape memory polymers for active cell culture

形状记忆聚合物（SMPS）是一类的“智能”材料，有能力从一个固定的，临时的形状改变预先确定的形状后应用的刺激， 如热1-5永久的。 SMP是一个典型的形状记忆周期，首先在比其转变温度T 反 [熔融温度（T 米 ）或玻璃化转变温度（Tg）]更高的高温变形。变形是弹性的性质和主要信息的构成的网络链（以下橡胶弹性理论）的构象熵的减少。变形的SMP，然后冷却到低于其T反温度，同时保持外部的应变或应力常数。在冷却过程中，材料过渡到更严格的状态（半结晶或玻璃状），其中动力学陷阱或“冻结”在这种低熵状态，导致宏观形状固定的材料。触发形状恢复是通过不断加热材料通过T 跨下一个无压力的条件（无约束）。使连锁网络（收复流动）放宽到其热力学的青睐，最大熵的状态，从临时形状的重大变化的永久的形状。 细胞是能够测量的力学性能及其周边环境6。机械细胞之间的相互作用和他们的物理环境控制细胞的行为的机制，通过活跃的研究领域。定义地形基板已成为功能强大的工具，在这些机制的调查。尺度，微观和纳米基板地形模式，已被证明直接单元格对齐方式，细胞粘附和细胞牵引力7-14。这些发现强调基板地形控制和检测机械在细胞培养细胞和它们的物理环境之间的相互作用的潜力，但日期基板一般都被动，不能编程的显着变化，在文化。这个物理瘀有限地形基板，以控制细胞培养的潜力。 在这里，活跃的细胞培养（ACC），SMP的基板介绍，聘请的表面形状记忆提供编程控制基板地形和变形。这些基板展示从一个临时的沟槽地形过渡到第二个，近持平记忆地形的能力。这种地形的变化，可以用来控制细胞标准的细胞培养条件下的行为。

Watch this Scientific Journal Video about Shape Memory Polymers for Active Cell Culture at JoVE.com

Shape Memory Polymers for Active Cell Culture

一个能力地形变化，在文化发展与细胞培养基质的方法是描述。该方法使被称为形状记忆聚合物，有能力记住一个永久的形状的智能材料的使用。这个概念是适应的材料和应​​用的广泛。

形状记忆聚合物主动细胞培养

Shape memory polymers (SMPs) are a class of "smart" materials that have the ability to change from a fixed, temporary shape to a pre-determined permanent ...

Research

JoVE Journal

Bioengineering

Microfabricated Platforms for Mechanically Dynamic Cell Culture

微机械动态细胞培养平台

The ability to systematically probe in vitro cellular response to combinations of mechanobiological stimuli for tissue engineering, drug discovery or ...

科研

生物工程

Rotating Cell Culture Systems for Human Cell Culture: Human Trophoblast Cells as a Model

旋转的人类细胞培养的细胞培养系统：作为一种模式的滋养细胞

The field of human trophoblast research aids in  understanding the complex environment established during placentation. Due to the  nature of these ...

Self-reporting Scaffolds for 3-Dimensional Cell Culture

自我报告的支架3维细胞培养

Culturing cells in 3D on appropriate scaffolds is thought to better mimic the in vivo microenvironment and increase cell-cell interactions. The resulting ...

Microfluidic Fabrication of Polymeric and Biohybrid Fibers with Predesigned Size and Shape

预设计尺寸和形状的聚合物纤维和生物贿赂纤维的微流体制造

A &#8220;sheath&#8221; fluid passing through a microfluidic channel at low Reynolds number can be directed around another &#8220;core&#8221; stream and ...

In vitro Cell Culture Model for Toxic Inhaled Chemical Testing

In vitro Cell Culture Model for Toxic Inhaled Chemical Testing

对于中吸入有毒化学测试体外细胞培养模型

Cell cultures are indispensable to develop and study efficacy of therapeutic agents, prior to their use in animal models. We have the unique ability to ...

Fabrication of a Bioactive, PCL-based "Self-fitting" Shape Memory Polymer Scaffold

生物活性，PCL为基础的“自我装配”形状记忆聚合物支架的制备

Tissue engineering has been explored as an alternative strategy for the treatment of critical-sized cranio-maxillofacial (CMF) bone defects. Essential to ...

Optical Control of Living Cells Electrical Activity by Conjugated Polymers

由共轭聚合物活细胞电活动的光控制

Hybrid interfaces between organic semiconductors and living tissues represent a new tool for in-vitro and in-vivo applications. In particular, conjugated ...

High-throughput Identification of Bacteria Repellent Polymers for Medical Devices

医疗器械细菌高通量鉴定驱蚊聚合物

Medical devices are often associated with hospital-acquired infections, which place enormous strain on patients and the healthcare system as well as ...

Engineering Molecular Recognition with Bio-mimetic Polymers on Single Walled Carbon Nanotubes

工程分子识别与单壁碳纳米管仿生聚合物

Semiconducting single-wall carbon nanotubes (SWNTs) are a class of optically active nanomaterial that fluoresce in the near infrared, coinciding with the ...

Tunable Hydrogels from Pulmonary Extracellular Matrix for 3D Cell Culture

可调水凝胶由肺细胞外基质的三维细胞培养

Here we present a method for establishing multiple component cell culture hydrogels for in vitro lung cell culture. Beginning with healthy en bloc lung ...