Name: modeling neural immune signaling of episodic and chronic migraine using spreading depression in vitro
Uploaded: 2011-06-13T13:00:00
Duration: 16 min 13 s
Description: Watch this Scientific Journal Video about Modeling Neural Immune Signaling of Episodic and Chronic Migraine Using Spreading Depression In Vitro at JoVE.com

这项工作是由国家神经紊乱和中风研究所（NS - 19108），全国儿童健康与疾病研究所（PO1 - HD09402），偏头痛研究基金会和白基金会的赠款支持。玛西娅体育Kraig女士的协助下在编制和维护文化系统。

<ol>
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SD是阵发性脑扰动，其在所有物种和大脑区域高度保守的特点研究迄今。 SD通常是在大脑皮层的研究。然而，这种结构的神经回路的复杂性（海马），以及无法保持大脑皮层长期时期的静态免疫功能的体外存活，在体外制备它作为一个有用的定义的长期后果SD的易感性。相比之下，海马不仅是大脑的结构是最敏感到SD的，它也是一个更简化的神经回路的结构和功能，这是适合定义神经活动可以调节的SD易感性neuroimmune手段archicortical结构。

modeling neural immune signaling of episodic and chronic migraine using spreading depression in vitro

偏头痛和慢性偏头痛的转变是需要改进治疗方案的医疗负担。我们寻求定义神经免疫信号调制易患偏头痛为蓝本， 在体外使用扩散性抑制（SD）作为一种手段来发展新的治疗靶点，为阵发性和慢性偏头痛。 SD是偏头痛先兆偏头痛的可能原因。它是一个由最初增加神经元的活动，慢慢易受影响的大脑区域内传播引发的神经功能阵发性的损失。正常的脑功能是极其敏感，依赖，同步低层次的免疫信号。因此，神经免疫信号可能会影响电活动的SD，因此，偏头痛。 SD疼痛的感知，在整个动物的研究困难重重，但很适合整体动物研究偏头痛的系统生物学方面，因为SD激活三叉神经的痛觉通路。不过，整体动物研究本身不能被用来破译的SD细胞和神经回路机制。相反， 在体外可以控制的环境条件下的准备工作是必要的。在这里，重要的是要承认急性切片和海马脑片培养具有明显的优势的局限性。急性脑切片不能透露，因为准备的片单独触发免疫信号的微妙的变化：亲炎性改变，最后几天，癫痫行为由于高氧张力的水平，需要振兴的切片，和不可逆的细胞损伤，缺氧片中心。 相比之下，我们在成熟的海马脑片培养的免疫信号，因为文化紧密平行在体内对口，具有成熟的trisynaptic功能;显示静态的星形胶质细胞，小胶质细胞和细胞因子水平; SD很容易诱发unanesthetized准备。此外，片长寿命和SD可以连续两天无损伤引起的，这种准备的唯一手段日期建模慢性SD neuroimmune后果，因此也许是慢性偏头痛。我们使用的电生理技术和非侵入性的成像测量神经细胞和电路功能与SD重合。神经免疫基因的表达变量定量PCR筛选，qPCR的阵列，cDNA的前置放大，更重要的是，使用超低水平，如γ-干扰素使用整个区域，或特定的细胞增强的目标检测与测量（通过激光清扫显微镜）抽样检验。细胞因子的级联信号的进一步评估复磷相关的目标与基因的表达，并通过细胞特异性免疫证实磷的变化。药理和siRNA的策略是用来模拟和调节免疫信号的SD 。

Watch this Scientific Journal Video about Modeling Neural Immune Signaling of Episodic and Chronic Migraine Using Spreading Depression In Vitro at JoVE.com

Modeling Neural Immune Signaling of Episodic and Chronic Migraine Using Spreading Depression In Vitro

偏头痛和慢性偏头痛的转变是巨大的医疗负担，需要改进治疗方案。我们寻求神经免疫信号调制易患偏头痛如何定义，建模<em&gt;在体外</em使用在海马脑片培养扩散性抑制，作为一种手段，以开发新的治疗靶点。

使用扩频萧条的情节和慢性偏头痛的神经免疫信号建模<em在体外</em

Migraine and its transformation to chronic migraine are healthcare burdens in need of improved treatment options.  We seek to define how neural immune ...

Research

JoVE Journal

Neuroscience

Modeling Neural Immune Signaling of Episodic and Chronic Migraine Using Spreading Depression In Vitro

A Thin-skull Window Technique for Chronic Two-photon In vivo Imaging of Murine Microglia in Models of Neuroinflammation

A Thin-skull Window Technique for Chronic Two-photon In vivo Imaging of Murine Microglia in Models of Neuroinflammation

一个慢性双光子薄颅骨窗口技术在体内 Neuroinflammation模型小鼠小胶质细胞成像

Traditionally in neuroscience, in vivo two photon imaging of the murine central nervous system has either involved the use of open-skull1,2 or ...

科研

神经科学

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睾酮和社会决策，结合行为内分泌和实验经济学：

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用于记录单机活动和Electrocorticographic信号的慢性神经电极植入手术

The success of long-term electrophysiological recordings often depends on the quality of the implantation surgery.  Here we provide useful information for ...

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设计和组装的超轻型电动微型硬盘（Microdrive）慢性神经录音的小动物

The ability to chronically record from populations of neurons in freely behaving animals has proven an invaluable tool for dissecting the function of ...

Modeling Astrocytoma Pathogenesis In Vitro and In Vivo Using Cortical Astrocytes or Neural Stem Cells from Conditional, Genetically Engineered Mice

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建模星形细胞瘤发病机制<em&gt;在体外</em&gt;和<em&gt;体内</em&gt;使用星形胶质细胞或条件，遗传工程小鼠神经干细胞

Current astrocytoma models are limited in their ability to define the roles of oncogenic mutations in specific brain cell types during disease ...

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神经细胞类型的流式细胞仪协议的表面和细胞内抗原分析

Flow cytometry has been extensively used to define cell populations in immunology, hematology and oncology. Here, we provide a detailed description of ...

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In Vitro Modeling of Cancerous Neural Invasion: The Dorsal Root Ganglion Model

在癌神经侵袭的体外模型：背根神经节型号

One way that solid tumors disseminate is through neural invasion. This route is well-known in cancers of the head and neck, prostate, and pancreas. These ...

Culturing In Vivo-like Murine Astrocytes Using the Fast, Simple, and Inexpensive AWESAM Protocol

Culturing In Vivo-like Murine Astrocytes Using the Fast, Simple, and Inexpensive AWESAM Protocol

使用快速、简单且便宜的 AWESAM 协议培养在体内像小鼠星形胶质细胞

The AWESAM (a low-cost easy stellate astrocyte method) protocol entails a fast, simple, and inexpensive way to generate large quantities of in vivo-like ...

Chronic Implantation of Whole-cortical Electrocorticographic Array in the Common Marmoset

常见马莫塞的全皮质电皮质阵列的慢性植入

Electrocorticography (ECoG) allows the monitoring of electrical field potentials from the cerebral cortex with high spatiotemporal resolution. Recent ...

Combining Behavior and EEG to Study the Effects of Mindfulness Meditation on Episodic Memory

结合行为和脑电图研究正念冥想对情景记忆的影响

Although there has been recent interest in how mindfulness meditation can affect episodic memory as well as brain structure and function, no study has ...