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DOI :
10.3791/2941-v
•
12:03 min
July 25th, 2011
Chapters
0:05
Title
1:46
Cell Lines, Vector Construction, and Lentiviral Production
3:19
Production of Head and Neck Cell Lines with Stable LifeAct-mCherry Expression
4:30
Orthotopic Tumor Xenograft Formation
5:56
Preparation of Mouse Tongues for Ex-vivo Imaging
6:27
Imaging of Tongue Tumors with Two-photon Microscopy and Image Analysis
9:55
Representative Tumor Images
11:04
Conclusion
产生的口腔癌和肿瘤浸润的定量监测的小鼠模型,通过多光子显微镜标记细胞内的舌头所涉及的技术的一个全面的概述。该系统可以作为一个有用的平台和反侵入性化合物的分子评估药物疗效。
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