August 13th, 2011
•We describe a low cost, high throughput method to screen for fungal endoglucanase activity in E. coli. The method relies on a simple visual readout of substrate degradation, does not require enzyme purification, and is highly scalable. This allows for the rapid screening of large libraries of enzyme variants.
Tags
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved