Name: introduction to the ultrasound targeted microbubble destruction technique
Uploaded: 2011-06-12T16:00:00
Description: Watch this Scientific Journal Video about Introduction to the Ultrasound Targeted Microbubble Destruction Technique at JoVE.com

格兰特支持包括NHLBI HL080532，NHLBI HL073449，NCRR RR16453的AHA国家批准的援助奖（RVS）。特别感谢延长距离的课程设计和咨询组（DCDC），dcdcgroup.org，协助视频制作和美国能源部的教育补助金号P336C050047创办的DCDC。

1。微气泡的股票准备<ol><li>在10毫升的PBS混合1克葡萄糖200毫克1,2 - 二棕榈酰- SN -甘油-3 - 磷脂和50毫克1,2 - 二棕榈酰- SN -甘油-3 - 磷脂。 </li><li>在沸水浴20-30分钟中加热的混合物，吸管搅拌，每隔5分钟。 </li><li>该解决方案可以存储在4℃至6个月。 </li></ol> 2。微泡的制备<ol><li>以250μL的准备微泡造影剂原液和孵化，在40 ° 15分钟。 </li><li>预热的微泡造影剂的解决方案，然后转移?...

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	<li>Bekeredjian, R., Chen, S., Frenkel, P. A., Grayburn, P. A., Shohet, R. V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Ultrasound-targeted+microbubble+destruction+can+repeatedly+direct+highly+specific+plasmid+expression+to+the+heart.">Ultrasound-targeted microbubble destruction can repeatedly direct highly specific plasmid expression to the heart.</a> Circulation. 108, 1022-1026 (2003).</li><li>Bekeredjian, R., Katus, H. A., Kuecherer, H. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Therapeutic+use+of+ultrasound+targeted+microbubble+destruction:+a+review+of+non-cardiac+applications.">Therapeutic use of ultrasound targeted microbubble destruction: a review of non-cardiac applications.</a> Ultraschall Med. 27, 134-140 (2006).</li><li>Chen, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Regeneration+of+pancreatic+islets+in+vivo+by+ultrasound-targeted+gene+therapy.">Regeneration of pancreatic islets in vivo by ultrasound-targeted gene therapy.</a> Gene Ther. 17, 1411-1420 (2010).</li><li>Miao, C. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Ultrasound+enhances+gene+delivery+of+human+factor+IX+plasmid.">Ultrasound enhances gene delivery of human factor IX plasmid.</a> Hum Gene Ther. 16, 893-905 (2005).</li><li>Shimoda, M., Chen, S., Noguchi, H., Matsumoto, S., Grayburn, P. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In+vivo+non-viral+gene+delivery+of+human+vascular+endothelial+growth+factor+improves+revascularisation+and+restoration+of+euglycaemia+after+human+islet+transplantation+into+mouse+liver.">In vivo non-viral gene delivery of human vascular endothelial growth factor improves revascularisation and restoration of euglycaemia after human islet transplantation into mouse liver.</a> Diabetologia. 53, 1669-1679 (2010).</li><li>Shohet, R. V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Echocardiographic+destruction+of+albumin+microbubbles+directs+gene+delivery+to+the+myocardium.">Echocardiographic destruction of albumin microbubbles directs gene delivery to the myocardium.</a> Circulation. 101, 2554-2556 (2000).</li><li>Sirsi, S., Borden, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Microbubble+Compositions,+Properties+and+Biomedical+Applications.">Microbubble Compositions, Properties and Biomedical Applications.</a> Bubble Sci Eng Technol. 1, 3-17 (2009).</li><li>Li, H. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Ultrasound-targeted+microbubble+destruction+enhances+AAV-mediated+gene+transfection+in+human+RPE+cells+in+vitro+and+rat+retina+in+vivo.">Ultrasound-targeted microbubble destruction enhances AAV-mediated gene transfection in human RPE cells in vitro and rat retina in vivo.</a> Gene Ther. 16, 1146-1153 (2009).</li><li>Lindner, J. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Microbubbles+in+medical+imaging:+current+applications+and+future+directions.">Microbubbles in medical imaging: current applications and future directions.</a> Nat Rev Drug Discov. 3, 527-532 (2004).</li><li>Newman, C. M., Bettinger, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Gene+therapy+progress+and+prospects:+ultrasound+for+gene+transfer.">Gene therapy progress and prospects: ultrasound for gene transfer.</a> Gene Ther. 14, 465-475 (2007).</li><li>Vancraeynest, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Myocardial+injury+induced+by+ultrasound-targeted+microbubble+destruction:+evidence+for+the+contribution+of+myocardial+ischemia.">Myocardial injury induced by ultrasound-targeted microbubble destruction: evidence for the contribution of myocardial ischemia.</a> Ultrasound Med Biol. 35, 672-679 (2009).</li></ol>

UTMD代表一个基因传递的新方法。作为一个平台技术，它可以与许多潜在的基因治疗策略相结合，提供无数时需要高度的组织特异性的生物活性分子。生物技术的主要限制是转染效率低。另一个重要的考虑是方便的靶器官超声，可显着减少干预骨或空气。该技术需要优化技术的基础上靶组织11以及声学的一个基本的了解，以限制组织损伤 11，提高工作效率。但是，它提供了一种廉?...

<table border="1"><tbody><tr><th>试剂/设备名称</th><th>公司</th><th>目录编号</th><th>评论</th></tr><tr><td> 1,2 - 二棕榈酰- SN -甘油-3 - 磷脂</td><td> Sigma - Aldrich公司</td><td> P - 5911 </td><td>微泡造影剂的脂质外壳组件</td></tr><tr><td> 1,2 - 二棕榈酰- SN -甘油-3 - 磷脂</td><td> Sigma - Aldrich公司</td><td> P - 3275 </td><td>微泡造影剂的脂质外壳组件</td></tr><tr><td>葡萄糖</td><td> Sigma - Aldrich公司</td><td> G5400 </td><td>认为稳定微泡</td></tr><tr><td>磷酸盐缓冲液</td><td> Sigma - Aldrich公司</td><td> P5368 </td><td></td></tr><tr><td>甘油</td><td> Sigma - Aldrich公司</td><td> G5516 </td><td>相信，以防止凝聚微泡</td></tr><tr><td> Octafluoropropane气体</td><td>公司Airgas </td><td> N / A </td><td>在临床应用中所用的惰性气体</td></tr><tr><td> VialMix牙科混汞</td><td>施贵宝</td><td> N / A </td><td></td></tr><tr><td> 1 MHz时，为13mm，重点不突出传感器</td><td>奥林巴斯</td><td> A303S - SU </td><td></td></tr><tr><td> 20 MHz函数/任意波形发生器</td><td>安捷伦</td><td> 33220A </td><td></td></tr><tr><td>功率放大器</td><td>克罗恩-海特有限公司</td><td>型号7500 </td><td></td></tr><tr><td>水听器</td><td> Brüel和克亚尔的</td><td>类型1803 </td><td></td></tr><tr><td>电荷放大器</td><td> Brüel和克亚尔的</td><td>类型2634 </td><td></td></tr><tr><td> 500 MHz示波器</td><td>力科</td><td> 9354L </td><td></td></tr><tr><td> 2100 VisualSonics“VEVO 34兆赫传感器的成像系统</td><td> VisualSonics </td><td> 2100 </td><td></td></tr><tr><td> 27G一英寸尾静脉导管</td><td> VisualSonics </td><td> N / A </td><td></td></tr><tr><td>精灵加输液泵</td><td>肯特科学</td><td> GENIE </td><td></td></tr></tbody></table>

introduction to the ultrasound targeted microbubble destruction technique

UTMD，生物活性分子，如带负电荷的质粒DNA编码感兴趣的基因的载体，添加阳离子脂质微泡造影剂7-9炮弹。在小鼠中，这些载体携带的微泡可静脉给药，或直接向心脏的左心室。在较大的动物，他们也可以通过冠状动脉导管注入。随后的交付，从流通到靶器官，发生在微泡的共振频率的声空化。这很可能是产生机械能在短暂的或有针对性的地区的10个微血管内皮细胞之间的孔隙形成的微气泡的破坏结果。由于这种sonoporation效应的结果，和跨内皮细胞的转染效率的提高，和转基因的编码向量存放到周围组织。质粒DNA留在流通迅速降解血液中的核酸交货的可能性，从而进一步降低非超声组织和高度特异性的靶器官转。

Watch this Scientific Journal Video about Introduction to the Ultrasound Targeted Microbubble Destruction Technique at JoVE.com

Introduction to the Ultrasound Targeted Microbubble Destruction Technique

超声针对性的微泡销毁（UTMD）可用于直接站点特定的生物活性分子的交付，包括基因治疗的靶器官，如心脏和肝脏超声，<sup&gt; 1-6</sup&gt;。

有针对性的超声微泡销毁技术简介

In UTMD, bioactive molecules, such as negatively charged plasmid DNA vectors encoding a gene of interest, are added to the cationic shells of lipid ...

Research

JoVE Journal

Bioengineering

Contrast Ultrasound Targeted Treatment of Gliomas in Mice via Drug-Bearing Nanoparticle Delivery and Microvascular Ablation

超声造影有针对性的治疗胶质瘤的小鼠通过药物轴承纳米粒子交付和微血管消融

We are developing minimally-invasive contrast agent microbubble based therapeutic approaches in which the permeabilization and/or ablation of the ...

科研

生物工程

Design of a Cyclic Pressure Bioreactor for the Ex Vivo Study of Aortic Heart Valves

Design of a Cyclic Pressure Bioreactor for the Ex Vivo Study of Aortic Heart Valves

循环压力为生物反应器的设计体外主动脉心脏瓣膜的研究

The aortic valve, located between the left ventricle and the aorta, allows for unidirectional blood flow, preventing backflow into the ventricle. Aortic ...

Analysis of Targeted Viral Protein Nanoparticles Delivered to HER2+ Tumors

分析有针对性的病毒蛋白的纳米粒子递送HER2 +肿瘤

The HER2+ tumor-targeted nanoparticle, HerDox, exhibits tumor-preferential accumulation and tumor-growth ablation in an animal model of HER2+ cancer. ...

Manufacture of Concentrated, Lipid-based Oxygen Microbubble Emulsions by High Shear Homogenization and Serial Concentration

制造浓缩，脂质为基础的氧气微泡的乳液由高剪切均质化和串行集中

Gas-filled microbubbles have been developed as ultrasound contrast and drug delivery agents. Microbubbles can be produced by processing surfactants using ...

A Microfluidic Technique to Probe Cell Deformability

微流体技术，探讨细胞变形

Here we detail the design, fabrication, and use of a microfluidic device to evaluate the deformability of a large number of individual cells in an ...

A Microfluidic Chip for ICPMS Sample Introduction

微流体芯片ICPMS进样

This protocol discusses the fabrication and usage of a disposable low cost microfluidic chip as sample introduction system for inductively coupled plasma ...

Synthesis of Gold Nanoparticle Integrated Photo-responsive Liposomes and Measurement of Their Microbubble Cavitation upon Pulse Laser Excitation

在脉冲激光激发金纳米粒子集成光电响应脂质体及其微泡空化的测量的合成

Photo-responsive nanoparticles (NPs) have received considerable attention because of their potential in providing spatial, temporal, and dosage control ...

An Experimental Protocol for Assessing the Performance of New Ultrasound Probes Based on CMUT Technology in Application to Brain Imaging

基于 CMUT 技术的新型超声探头在脑成像中的应用研究

The possibility to perform an early and repeatable assessment of imaging performance is fundamental in the design and development process of new ...

Comprehensive Evaluation of the Effectiveness and Safety of Placenta-Targeted Drug Delivery Using Three Complementary Methods

用三种互补方法综合评价胎盘靶向药物的有效性和安全性

No effective treatments currently exist for placenta-associated pregnancy complications, and developing strategies for the targeted delivery of drugs to ...

Preparing Protein Producing Synthetic Cells using Cell Free Bacterial Extracts, Liposomes and Emulsion Transfer

使用无细胞细菌提取物、脂质体和乳液转移制备蛋白质生成合成细胞

The bottom-up assembly approach for construction of synthetic cells is an effective tool for isolating and investigating cellular processes in a cell ...