Name: introduction to the ultrasound targeted microbubble destruction technique
Uploaded: 2011-06-12T16:00:00
Description: Watch this Scientific Journal Video about Introduction to the Ultrasound Targeted Microbubble Destruction Technique at JoVE.com

Hibe desteği NHLBI HL080532, NHLBI HL073449 NCRR RR16453 ve Hibe Yardım Ödülü (RVS) AHA Milli dahil etmiştir. Mesafe Ders Tasarım ve Danışmanlık (DCDC) grubu, dcdcgroup.org, video üretimi ile yardım için özel bir teşekkür ve DCDC kurdu Eğitim Bursu No P336C050047 US Department of uzatılır.

1. Mikro stok hazırlama <ol><li> 10 ml PBS 1 gr glukoz 200 mg 1,2-dipalmitoyl-sn Glycéro-3-fosfatidilkolin ve 50 mg 1,2 dipalmitoyl-sn-Glycéro-3-phosphatidylethanolamine karıştırın. </li><li> Pipet her 5 dakikada bir karıştırma, karışım kaynar su banyosunda 20-30 dakika ısıtın. </li><li> Çözüm, 6 aya kadar 4 ° C saklanabilir. </li></ol> 2. Mikro Hazırlık <ol><li> 40 mikrokabarcık hazırlanan stok solüsyonu ve inkübe ° C 15 dakika boyunca 250 ul çekin. </li><li> Sonr...

<ol>
	<li>Bekeredjian, R., Chen, S., Frenkel, P. A., Grayburn, P. A., Shohet, R. V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Ultrasound-targeted+microbubble+destruction+can+repeatedly+direct+highly+specific+plasmid+expression+to+the+heart.">Ultrasound-targeted microbubble destruction can repeatedly direct highly specific plasmid expression to the heart.</a> Circulation. 108, 1022-1026 (2003).</li><li>Bekeredjian, R., Katus, H. A., Kuecherer, H. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Therapeutic+use+of+ultrasound+targeted+microbubble+destruction:+a+review+of+non-cardiac+applications.">Therapeutic use of ultrasound targeted microbubble destruction: a review of non-cardiac applications.</a> Ultraschall Med. 27, 134-140 (2006).</li><li>Chen, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Regeneration+of+pancreatic+islets+in+vivo+by+ultrasound-targeted+gene+therapy.">Regeneration of pancreatic islets in vivo by ultrasound-targeted gene therapy.</a> Gene Ther. 17, 1411-1420 (2010).</li><li>Miao, C. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Ultrasound+enhances+gene+delivery+of+human+factor+IX+plasmid.">Ultrasound enhances gene delivery of human factor IX plasmid.</a> Hum Gene Ther. 16, 893-905 (2005).</li><li>Shimoda, M., Chen, S., Noguchi, H., Matsumoto, S., Grayburn, P. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In+vivo+non-viral+gene+delivery+of+human+vascular+endothelial+growth+factor+improves+revascularisation+and+restoration+of+euglycaemia+after+human+islet+transplantation+into+mouse+liver.">In vivo non-viral gene delivery of human vascular endothelial growth factor improves revascularisation and restoration of euglycaemia after human islet transplantation into mouse liver.</a> Diabetologia. 53, 1669-1679 (2010).</li><li>Shohet, R. V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Echocardiographic+destruction+of+albumin+microbubbles+directs+gene+delivery+to+the+myocardium.">Echocardiographic destruction of albumin microbubbles directs gene delivery to the myocardium.</a> Circulation. 101, 2554-2556 (2000).</li><li>Sirsi, S., Borden, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Microbubble+Compositions,+Properties+and+Biomedical+Applications.">Microbubble Compositions, Properties and Biomedical Applications.</a> Bubble Sci Eng Technol. 1, 3-17 (2009).</li><li>Li, H. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Ultrasound-targeted+microbubble+destruction+enhances+AAV-mediated+gene+transfection+in+human+RPE+cells+in+vitro+and+rat+retina+in+vivo.">Ultrasound-targeted microbubble destruction enhances AAV-mediated gene transfection in human RPE cells in vitro and rat retina in vivo.</a> Gene Ther. 16, 1146-1153 (2009).</li><li>Lindner, J. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Microbubbles+in+medical+imaging:+current+applications+and+future+directions.">Microbubbles in medical imaging: current applications and future directions.</a> Nat Rev Drug Discov. 3, 527-532 (2004).</li><li>Newman, C. M., Bettinger, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Gene+therapy+progress+and+prospects:+ultrasound+for+gene+transfer.">Gene therapy progress and prospects: ultrasound for gene transfer.</a> Gene Ther. 14, 465-475 (2007).</li><li>Vancraeynest, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Myocardial+injury+induced+by+ultrasound-targeted+microbubble+destruction:+evidence+for+the+contribution+of+myocardial+ischemia.">Myocardial injury induced by ultrasound-targeted microbubble destruction: evidence for the contribution of myocardial ischemia.</a> Ultrasound Med Biol. 35, 672-679 (2009).</li></ol>

UTMD gen teslimat için yeni bir yaklaşımı temsil ediyor. Bir teknoloji platformu olarak, doku özgüllüğü yüksek derecede istenilen biyoaktif moleküllerin sayısız sunmak için birçok potansiyel gen tedavisi stratejileri ile kombine edilebilir. Tekniğin ana biyolojik sınırlama transfeksiyon en düşük verim. Bir başka önemli husus, belirgin, kemik ya da hava müdahale ederek azalmış olabilir ultrason, hedef organ erişilebilirlik. Bu teknik, doku hasarı 11 ve verimliliği artırmak sınır...

<table border="1">
	<tbody>
		<tr>
			<th>Name of the reagent/equipment</th>
			<th>Company</th>
			<th>Catalogue number</th>
			<th>Comments</th>
		</tr>
		<tr>
			<td>1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine</td>
			<td>Sigma-Aldrich</td>
			<td>P-5911</td>
			<td>component of the microbubble lipid shell</td>
		</tr>
		<tr>
			<td>1,2-dipalmitoyl-sn-glycero-3-phosphatidylethanolamine</td>
			<td>Sigma-Aldrich</td>
			<td>P-3275</td>
			<td>component of the microbubble lipid shell</td>
		</tr>
		<tr>
			<td>glucose</td>
			<td>Sigma-Aldrich</td>
			<td>G5400</td>
			<td>thought to stabilize the microbubbles</td>
		</tr>
		<tr>
			<td>phosphate-buffered saline</td>
			<td>Sigma-Aldrich</td>
			<td>P5368</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>glycerol</td>
			<td>Sigma-Aldrich</td>
			<td>G5516</td>
			<td>believed to prevent microbubbles from coalescing</td>
		</tr>
		<tr>
			<td>Octafluoropropane gas</td>
			<td>AirGas</td>
			<td>N/A</td>
			<td>inert gas used in clinical applications</td>
		</tr>
		<tr>
			<td>VialMix dental amalgamator</td>
			<td>Bristol-Myers Squibb</td>
			<td>N/A</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>1 MHz, 13mm, unfocused transducer</td>
			<td>Olympus</td>
			<td>A303S-SU</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>20 MHz Function/Arbitrary Waveform Generator</td>
			<td>Agilent</td>
			<td>33220A</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>Power Amplifier</td>
			<td>Krohn-Hite Co.</td>
			<td>Model 7500</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>Hydrophone</td>
			<td>Bruel and Kjaer</td>
			<td>Type 1803</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>Charge Amplifier</td>
			<td>Bruel and Kjaer</td>
			<td>Type 2634</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>500 MHz Oscilloscope</td>
			<td>LeCroy</td>
			<td>9354L</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>VisualSonics&#x2019; Vevo 2100 Imaging System with 34 MHz transducer</td>
			<td>VisualSonics</td>
			<td>2100</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>27G one inch tail vein catheters</td>
			<td>VisualSonics</td>
			<td>N/A</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>Genie Plus infusion pump</td>
			<td>Kent Scientific</td>
			<td>GENIE</td>
			<td>&nbsp;</td>
		</tr>
	</tbody>
</table>

introduction to the ultrasound targeted microbubble destruction technique

Gibi negatif yüklü ilgi bir gen kodlayan plazmid DNA vektörleri UTMD, biyoaktif moleküllerin lipid mikrokabarcık kontrast ajanlar 7-9 katyonik kabukları eklenir. Farelerde bu vektör taşıyan mikro kabarcık kalbin sol ventrikül intravenöz veya doğrudan uygulanabilir. Büyük hayvanlarda onlar da intrakoroner bir kateter aracılığıyla infüze olabilir. Dolaşımdan bir hedef organ sonraki teslim mikro kabarcık rezonans frekansta akustik kavitasyon oluşur. Bu mekanik enerji, ya da hedef bölgedeki 10 mikrovasküler endotel hücreleri arasındaki geçici gözenek oluşumuna mikrokabarcık imha sonuçları tarafından üretilen olası görünüyor . Bu sonoporation etkinin bir sonucu olarak, gelişmiş ve endotel hücreleri arasında transfeksiyon verimliliği ve transgen kodlama vektörler çevreleyen doku içine yatırılır. Nükleazlar-sonicated olmayan dokulara daha teslim olasılığını azaltır ve oldukça belirli bir hedef organ transfeksiyon yol açar, kan dolaşımını kalan plazmid DNA hızla düşer.

Watch this Scientific Journal Video about Introduction to the Ultrasound Targeted Microbubble Destruction Technique at JoVE.com

Introduction to the Ultrasound Targeted Microbubble Destruction Technique

Ultrason Hedeflenen Mikro Destruction (UTMD), ultrason ile kalp ve karaciğer gibi organlar, hedef, terapötik genler de dahil olmak üzere, biyoaktif moleküllerin siteye özgü teslimat, doğrudan kullanılabilir<sup&gt; 1-6</sup&gt;.

Ultrason Hedeflenen Mikro İmha Tekniği Giriş

In UTMD, bioactive molecules, such as negatively charged plasmid DNA vectors encoding a gene of interest, are added to the cationic shells of lipid ...

Research

JoVE Journal

Bioengineering

Contrast Ultrasound Targeted Treatment of Gliomas in Mice via Drug-Bearing Nanoparticle Delivery and Microvascular Ablation

Kontrast Ultrason Gliomlar, Tedavi Hedeflenen İlaç-Rulman Nanopartikül Teslimat ve Mikrovasküler Ablasyon ile Fare

We are developing minimally-invasive contrast agent microbubble based therapeutic approaches in which the permeabilization and/or ablation of the ...

Design of a Cyclic Pressure Bioreactor for the Ex Vivo Study of Aortic Heart Valves

Design of a Cyclic Pressure Bioreactor for the Ex Vivo Study of Aortic Heart Valves

Devirli Basınç Biyoreaktör Tasarım Ex vivo</emAort Kalp Vanalar> Eğitim

The aortic valve, located between the left ventricle and the aorta, allows for unidirectional blood flow, preventing backflow into the ventricle. Aortic ...

Analysis of Targeted Viral Protein Nanoparticles Delivered to HER2+ Tumors

Hedefli Viral Protein analizi HER2 + tümörler için teslim Nanopartiküller

The HER2+ tumor-targeted nanoparticle, HerDox, exhibits tumor-preferential accumulation and tumor-growth ablation in an animal model of HER2+ cancer. ...

Manufacture of Concentrated, Lipid-based Oxygen Microbubble Emulsions by High Shear Homogenization and Serial Concentration

Yüksek homojenizasyon ve Seri Konsantrasyon konsantre, Lipid-tabanlı Oksijen Mikro Emülsiyonlar imalatı

Gas-filled microbubbles have been developed as ultrasound contrast and drug delivery agents. Microbubbles can be produced by processing surfactants using ...

A Microfluidic Technique to Probe Cell Deformability

Mikroakışkan Tekniği Hücre deformabilitesi Probe

Here we detail the design, fabrication, and use of a microfluidic device to evaluate the deformability of a large number of individual cells in an ...

A Microfluidic Chip for ICPMS Sample Introduction

ICPMS Numune Giriş için mikroakışkan Chip

This protocol discusses the fabrication and usage of a disposable low cost microfluidic chip as sample introduction system for inductively coupled plasma ...

Synthesis of Gold Nanoparticle Integrated Photo-responsive Liposomes and Measurement of Their Microbubble Cavitation upon Pulse Laser Excitation

Darbe Lazer Tahrik olma üzerine Altın Nanopartikül Entegre Fotoğraf duyarlı lipozomlar ve Bunların Mikro-kabarcık Kavitasyon Ölçülmesi Sentezi

Photo-responsive nanoparticles (NPs) have received considerable attention because of their potential in providing spatial, temporal, and dosage control ...

An Experimental Protocol for Assessing the Performance of New Ultrasound Probes Based on CMUT Technology in Application to Brain Imaging

Beyin görüntüleme uygulamaya CMUT teknolojisi tabanlı yeni ultrason probları performansını değerlendirmek için deneysel bir iletişim kuralı

The possibility to perform an early and repeatable assessment of imaging performance is fundamental in the design and development process of new ...

Comprehensive Evaluation of the Effectiveness and Safety of Placenta-Targeted Drug Delivery Using Three Complementary Methods

Kapsamlı değerlendirme etkinliği ve güvenliği üç tamamlayıcı yöntemlerle plasenta hedefli ilaç dağıtım

No effective treatments currently exist for placenta-associated pregnancy complications, and developing strategies for the targeted delivery of drugs to ...

Preparing Protein Producing Synthetic Cells using Cell Free Bacterial Extracts, Liposomes and Emulsion Transfer

Hücre Serbest Bakteriyel Özler, Lipozomlar ve Emülsiyon Transferi Kullanarak Protein Üreten Sentetik Hücreler Hazırlama

The bottom-up assembly approach for construction of synthetic cells is an effective tool for isolating and investigating cellular processes in a cell ...