Name: diagnostic necropsy and selected tissue and sample collection in rats and mice
Uploaded: 2011-08-07T13:00:00
Description: Watch this Scientific Journal Video about Diagnostic Necropsy and Selected Tissue and Sample Collection in Rats and Mice at JoVE.com

1。剖检的准备工作<ol><li>任何总尸检（总尸检）的重要组成部分，是动物的历史和调查结果的描述。 </li><li>一个兽医病理学家读您的组织病理学幻灯片可能没有见过的动物，是靠你的背景资料。 </li><li>准确描述你所看到之前安乐死的动物。例如，“雌性小鼠在笼子里五之一; C57BL/6N;头部倾斜的权利”或“棕色的雄性大鼠应变未知;动物刮伤;有斑片状脱发足背附近的尾巴，和头“。 </li><li>使用清...

<ol>
	<li>AVMA. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> AVMA Guidelines on Euthanasia. , (2007).</li><li>Feldman, D. B., Seely, J. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Necropsy Guide: Rodents and the rabbit. , (1988).</li><li>King, J. M., Dodd, D. C., Roth, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> The Necropsy Book. , (2006).</li><li>Kittel, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Revised+guides+for+organ+sampling+and+trimming+in+rats+and+mice--Part+2.+A+joint+publication+of+the+RITA+and+NACAD+groups.">Revised guides for organ sampling and trimming in rats and mice--Part 2. A joint publication of the RITA and NACAD groups.</a> Exp Toxicol Pathol. 55, 413-431 (2004).</li><li>Komarek, V., Fox, J. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Chapter+1.">Chapter 1.</a> The Mouse in Biomedical Research. , 1-22 (2007).</li><li>Morawietz, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Revised+guides+for+organ+sampling+and+trimming+in+rats+and+mice--Part+3.+A+joint+publication+of+the+RITA+and+NACAD+groups.">Revised guides for organ sampling and trimming in rats and mice--Part 3. A joint publication of the RITA and NACAD groups.</a> Exp Toxicol Pathol. 55, 433-449 (2004).</li><li>Popesko, P., Raijtov&#225;, V., Hor&#225;k, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Colour Atlas of Anatomy of Small Laboratory Animals. 2, 91-106 (2002).</li><li>Ruehl-Fehlert, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Revised+guides+for+organ+sampling+and+trimming+in+rats+and+mice--part+1.">Revised guides for organ sampling and trimming in rats and mice--part 1.</a> Exp Toxicol Pathol. 55, 91-106 (2003).</li></ol>

在研究结束的数据收集可能需要的动物尸检。说明什么是见过，记得检查所有的组织。这些程序设计主要是为了优化剖检传染病的监测和样品采集，但最容易适应怀疑疾病的事件或聘用爆发也许只是一个程序的一个子集，将重点调查。很多手续也较常用在研究结束时，作为一个整体形态再加上收集特定的研究样本以供后续分析评估的一部分进行验尸有关。

<table border="1"><tbody><tr><th>试剂名称</th><th>公司</th><th>目录编号</th><th>评论</th></tr><tr><td>菜板</td><td>热电公司猫＃36114 </td><td> CAT＃36114 </td><td></td></tr><tr><td>小剪刀</td><td> ROBOZ </td><td> RS - 5910，G204 </td><td> 23毫米刀片，3.5“长，直</td></tr><tr><td>中等剪刀</td><td> ROBOZ </td><td> RS - 6808，G207 </td><td> 5“ </td></tr><tr><td>大剪刀</td><td> ROBOZ </td><td> RS - 6826，G65“ </td><td> 6.25“ </td></tr><tr><td>钳弯</td><td> ROBOZ </td><td> RS - 8254（M1/21004） </td><td> 4.5英寸，锯齿，轻微的曲线</td></tr><tr><td>镊子，Microdissecting </td><td> ROBOZ </td><td> RS - 5238 </td><td>哈德森（埃瓦尔德） </td></tr><tr><td>钳，组织钳</td><td> ROBOZ </td><td> RS - 8160 </td><td>鼠齿</td></tr><tr><td>火焰消毒器</td><td>牛津大学医学实验产品</td><td> Bacti的焚尸炉＃5889-001007 </td><td></td></tr><tr><td>解剖刀</td><td>癌症诊断， </td><td>手指解剖刀＃60，＃FS0060猫</td><td></td></tr><tr><td>移液器</td><td> VWR </td><td>巴斯德吸取5 3 / 4“，猫＃14672-400 </td><td></td></tr><tr><td>高压灭菌袋</td><td>正确的制造公司</td><td>灭菌袋纸包装袋猫＃021002（3100923）E09110 </td><td>对于巴氏吸管</td></tr><tr><td>移液器灯泡</td><td> VWR </td><td> CAT＃56310-240 </td><td></td></tr><tr><td> Eppendorf管</td><td> Sarstedt </td><td> SafeSeal微管2ML，楼盘编号72.695 </td><td></td></tr><tr><td> Eppendorf管</td><td>雅高</td><td> 5ML微管猫＃T20765 - C </td><td></td></tr><tr><td>注射器</td><td> BD（Beckton迪肯森） </td><td> 1毫升- 309602 3猫ML - 300910 5ML - 309603 10ML 20ML猫＃309604＃309661 </td><td></td></tr><tr><td>针</td><td>屋宇署</td><td> 26G - 309625 18G - 305195 </td><td></td></tr><tr><td>中性缓冲福尔马林</td><td> VWR </td><td> 20L NBF 10％，猫＃16004-128 </td><td></td></tr><tr><td>盐水</td><td> ThermoScientific </td><td>缓冲血库生理盐水，猫＃23-309-178 </td><td></td></tr><tr><td>胰酪胨大豆肉汤</td><td>流式细胞迪肯森</td><td> TSB：脱水​​，猫＃211825 </td><td></td></tr><tr><td>磷酸盐缓冲液</td><td>西格玛爱秩序</td><td>房委会的缓冲区，CAT＃P3813 - 10PAK </td><td></td></tr><tr><td>福尔马林杯</td><td> VWR </td><td> 4盎司猫＃36318-8528盎司猫＃36318-86016盎司猫＃36318-858 </td><td></td></tr><tr><td>大型福尔马林杯</td><td>橡树岭产品</td><td> 32盎司容器猫＃0432-1100 </td><td></td></tr><tr><td>超大福尔马林杯</td><td> VWR </td><td>高密度聚乙烯多用途160盎司容器猫＃89038-282 </td><td></td></tr><tr><td>缝合材料</td><td>亨利施恩</td><td>手术缝合线编织丝绸，楼盘编号100-5000，M766750 </td><td>对于鼠标肺胀</td></tr><tr><td>缠绕</td><td>斯台普斯</td><td>猫＃QUA - 46173 </td><td>对于大鼠肺胀</td></tr></tbody></table>

diagnostic necropsy and selected tissue and sample collection in rats and mice

有多个样品，可以收集从安乐死的动物，以帮助诊断或发现的动物群中的传染性病原体的类型。妥善收集进一步组织处理的组织可能会影响测试结果的质量。本文介绍了一个基本的总检查，包括识别心，肝，肺，肾，和脾，以及如何收集这些机关的行为。另外四个较困难的组织/样品采集技术演示。龙收集和灌注可以特别具有挑战性的组织需要正确的膨胀，在组织内部的秩序固定液适当的修正，以便彻底的组织学评价。本文演示了由一步一步的技术，以实现在24小时内组织的最佳固定删除肺和固定液膨胀。脑集合可以用同样的挑战，组织是软的，容易损坏。本文演示了通过一步一步的技术，从颅骨与脑组织损害最小的揭露和消除。肠系膜淋巴结是一个很好的样本类型，在其中发现许多常见的传染性病原体为肠道病毒持续在淋巴结，比他们在粪便棚。本文演示的定位，并在无菌条件下取出肠系膜淋巴结的一步一步程序。最后，呼吸道传染性病原体的鉴定可以由鼻腔和/或吸在剖检支气管液的细菌培养或PCR测试。此过程介绍了获取和准备进行细菌培养和PCR检测呼吸道吸出样品。

Watch this Scientific Journal Video about Diagnostic Necropsy and Selected Tissue and Sample Collection in Rats and Mice at JoVE.com

Diagnostic Necropsy and Selected Tissue and Sample Collection in Rats and Mice

本文介绍了进行基本的鼠标或大鼠尸检的程序，以及收集的基本器官，以及更多具有挑战性的样品类型，病理，微生物，和PCR评价。

剖检诊断，并选择在大鼠和小鼠的组织和样品收集

There are multiple sample types that may be collected from a euthanized animal in order to help diagnose or discover infectious agents in an animal ...

Research

JoVE Journal

Biology

Using Micro-Electro-Mechanical Systems (MEMS) to Develop Diagnostic Tools

Using Micro-Electro-Mechanical Systems MEMS to Develop Diagnostic Tools

使用微机电系统（MEMS）开发诊断工具

科研

生物学

Human Pancreatic Islet Isolation: Part I: Digestion and Collection of Pancreatic Tissue

人类胰岛的分离：第一部分：收集和胰腺组织消化

Management of Type 1 diabetes is burdensome, both to the individual and society, costing over 100 billion dollars annually. Despite the widespread use of ...

Seawater Sampling and Collection

海水采样和收集

This video documents methods for collecting coastal marine water samples and processing them for various downstream applications including biomass ...

Manual Restraint and Common Compound Administration Routes in Mice and Rats

手动约束和常见的化合物在小鼠和大鼠的给药途径

Being able to safely and effectively restrain mice and rats is an important part of conducting research.  Working confidently and humanely with mice and ...

Germ Cell Transplantation and Testis Tissue Xenografting in Mice

生殖细胞的移植和睾丸组织在小鼠异种移植

Germ cell transplantation was developed by Dr. Ralph Brinster and colleagues at the University of Pennsylvania in 19941,2. These ground-breaking studies ...

Ovariectomy and 17&beta;-estradiol Replacement in Rats and Mice: A Visual Demonstration

Ovariectomy and 17ÃƒÅ½Ã‚Â²-estradiol Replacement in Rats and Mice: A Visual Demonstration

在大鼠和小鼠的卵巢切除和17β-雌二醇更换：一个可视化示范

Estrogens are a family of female sexual hormones with an exceptionally wide spectrum of effects. When rats and mice are used in estrogen research they are ...

Proteomic Sample Preparation from Formalin Fixed and Paraffin Embedded Tissue

修正了福尔马林蛋白质组学样品的制备和石蜡包埋组织

Preserved clinical material is a unique source for proteomic investigation of human disorders. Here we describe an optimized protocol allowing large scale ...

Milk Collection Methods for Mice and Reeves' Muntjac Deer

牛奶收集方法小鼠和里夫斯&#39;麂鹿

Animal models are commonly used throughout research laboratories to accomplish what would normally be considered impractical in a pathogen&#8217;s native ...

Dissection of the Auditory Bulla in Postnatal Mice: Isolation of the Middle Ear Bones and Histological Analysis

在出生后的小鼠听泡解剖：中耳骨骼和组织学分析的分离

In most mammals, auditory ossicles in the middle ear, including the malleus, incus and stapes, are the smallest bones. In mice, a bony structure called ...

Cystometric and External Urethral Sphincter Measurements in Awake Rats with Implanted Catheter and Electrodes Allowing for Repeated Measurements

压和外尿道括约肌测量在清醒大鼠植入导管和电极允许重复测量

Lower urinary tract function is mainly assessed by means of cystometric bladder function analysis in rodents. Conventional cystometries are usually ...