Name: the application of permanent middle cerebral artery ligation in the mouse
Uploaded: 2011-07-25T13:00:00
Description: Watch this Scientific Journal Video about The Application Of Permanent Middle Cerebral Artery Ligation in the Mouse at JoVE.com

手术技术的最初收购的威廉D.山博士在乔治亚医学院的实验室。作者还要感谢大卫博士A. Rempe和兰大Prifti解剖相机使用。这项研究是由美国国立卫生研究院NS041744 NS051279，F31 NS064700和果酸30815697D支持。

该协议被批准用于对动物研究的伦理（UCAR）的大学罗切斯特委员会。在协议应遵循无菌技术。无菌手套和口罩的使用是必需的。 表1描述了所有的设备，材料，化学品，并在协议使用工具。 1。手术前准备<ol><li>注入小鼠皮下丁丙诺啡（0.05mg/kg），术前2小时后，立即手术，然后在第一个24小时后手术期每​​3-5 h。 </li><li>高压灭菌器消毒所有的手术器械?...

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永久性MCA结扎方法给予高度重复性的脑梗死体积和增加手术后的生存率率比现有的其他方法。程序的难易程度和持续时间短（约30分钟），使其更加实用。该方法被广泛用于在小鼠和大鼠。 这种方法需要一个立体显微镜下微创手术。因此，在显微镜下操作和完善一个成功的开颅手术的经验是必不可少的。最好是建立在实验室进行实验前，一致梗死。为了实现可重复性的结果是...

<table border="1"><tbody><tr><th>名称</th><th>公司联系号码</th></tr><tr><td> 答：解决方案和化学品 </td><td></td></tr><tr><td>碘伏溶液擦洗手术</td><td> Aplicare，82-209 </td></tr><tr><td> 70％的乙醇</td><td> Koptec，V1001 </td></tr><tr><td> 2,3,5 - 三苯基氯化氮唑（TTC） </td><td>西格玛，T8877 </td></tr><tr><td> 4％多聚甲醛</td><td>电子显微镜学，159SP </td></tr><tr><td>磷酸盐缓冲生理盐水，pH值：7.4 </td><td>在实验室中制造和消毒。 </td></tr><tr><td>矿物油</td><td> PML的Microbiologicals，R6570 </td></tr><tr><td> Isofluorane </td><td>巴特勒公司，029405 </td></tr><tr><td>丁丙诺啡（0.3mg/ml） </td><td> Hospira公司，NDC 0409-2012-32 </td></tr><tr><td>人工泪液</td><td>巴特勒公司，007312 </td></tr><tr><td>恢复凝胶</td><td>清除H 2 O 72-01-5022 </td></tr><tr><td> B.手术材料和设备 </td><td></td></tr><tr><td> C57/BL6鼠标，10至12周岁</td><td>殖民地大学动植物公园饲养</td></tr><tr><td> 100％的氧气罐</td><td>公司Airgas </td></tr><tr><td>麻醉蒸发器和流量计</td><td> Surgivet，型号100 </td></tr><tr><td>直肠探头的加热垫</td><td>精细的科学工具，TR200 </td></tr><tr><td>解剖显微镜</td><td> 2000年蔡司，STEMI </td></tr><tr><td>加热板</td><td> PETCO服务有限公司0307-013 </td></tr><tr><td>小血管Cauterizer集</td><td>精细的科学工具，18000-00 </td></tr><tr><td>迷你蠕动泵</td><td>哈佛器械，MPII </td></tr><tr><td> 1ml注射器</td><td>屋宇署，329650 </td></tr><tr><td> 18G针</td><td> Becton Dickinson公司，305196 </td></tr><tr><td>手术5.0尼龙缝线</td><td> Ethilon，698H </td></tr><tr><td>切片座</td><td>肯特科学，1毫米</td></tr><tr><td>单边刀片</td><td>电子显微镜学，71962 </td></tr><tr><td>标签胶带</td><td> Scienceware，倍儿最先进的产品F13463 - 2075 </td></tr><tr><td>纱布垫</td><td>肯德尔Curity 2252，约翰逊和约翰逊6415 </td></tr><tr><td>棉花提示喷枪</td><td>清教徒806 - WCL </td></tr><tr><td> C.手术工具 </td><td></td></tr><tr><td>手术弹簧剪刀</td><td>精细的科学工具15003-08 </td></tr><tr><td> Microdissecting钳角度</td><td>精细的科学工具5 / 45 </td></tr><tr><td> Microdissecting钳弯曲</td><td>精细的科学工具5钛</td></tr><tr><td>细直剪</td><td>精细的科学工具14568-12 </td></tr><tr><td>曲骨咬骨钳</td><td>精细的科学工具16021-14 </td></tr><tr><td>哈特曼止血钳弯</td><td>精细的科学工具13003-10 </td></tr></tbody></table> 表1。MCA结扎过程中使用的材料，工具和化学品清单。 A.解决方案和化学品，B.外科材料和设备，C.手术工具。

the application of permanent middle cerebral artery ligation in the mouse

局灶性脑缺血之间看到中风患者最常见的类型。由于临床意义出现了长时间的努力，制定合适的动物模型研究缺血期间开展的活动。这些技术包括短暂或永久的，局灶性或全球的许多不同的动物模型，使用最常见的啮齿类动物的缺血模型。 永久性MCA结扎的方法，这也是在文献中提到pMCAo，被广泛使用作为一个在啮齿类动物中1-6局灶性脑缺血模型。这种方法最初是老鼠田村等。在1981年7。开颅手术是在这个协议用于访问由电闭塞MCA和近端地区。梗死涉及大多是皮层和纹状体有时的地区，根据闭塞的位置。现在这项技术是建立和使用在许多实验室8-13。早期使用这种技术的定义和描述的“梗塞核心”和“半影”14日至16日，它往往是用来评估潜在的神经保护作用的化合物10，12，13， 17。虽然最初的研究是在大鼠进行的，永久的马华结扎已成功地使用在18-20稍作修改的小鼠。 这种模式产生重现梗死和增加后的存活率。在人类缺血性中风的约80％发生在MCA 面积 21，因此这是一个中风的研究非常相关的模型。目前，中风患者提供有效的治疗方法缺乏，因此需要有一个很好的模式，来测试潜在的药理的化合物和评估生理成果。这种方法也可以用于研究体内细胞缺氧反应机制。 在这里，我们目前在C57/BL6鼠标MCA结扎手术。我们描述的术前准备，马华结扎手术和2,3,5 - 三苯基氯化四氮唑（TTC）染色脑梗死体积的定量。

Watch this Scientific Journal Video about The Application Of Permanent Middle Cerebral Artery Ligation in the Mouse at JoVE.com

The Application Of Permanent Middle Cerebral Artery Ligation in the Mouse

大脑中动脉（MCA）结扎是一种技术，研究在动物模型局灶性脑缺血。在此方法中，通过开颅暴露大脑中动脉和烧灼结扎。此方法提供了高度重复性的脑梗死体积和增加手术后的生存率率比现有的其他方法。

在小鼠永久性大脑中动脉结扎术中的应用

Focal cerebral ischemia is among the most common type of stroke seen in patients. Due to the clinical significance there has been a prolonged effort to ...

Research

JoVE Journal

Medicine

Modeling Stroke in Mice - Middle Cerebral Artery Occlusion with the Filament Model

建模在小鼠体内的行程 - 中东与灯丝模型大脑中动脉闭塞

Stroke is among the most frequent causes of death and adult disability, especially in highly developed countries. However, treatment options to date are ...

科研

医学

Mouse Model of Middle Cerebral Artery Occlusion

大脑中动脉闭塞的小鼠模型

Stroke is the most common fatal neurological disease in the United States 1. The majority of strokes (88%) result from blockage of blood vessels in the ...

Utilizing a Cranial Window to Visualize the Middle Cerebral Artery During Endothelin-1 Induced Middle Cerebral Artery Occlusion

利用颅窗口，在可视化的大脑中动脉内皮素-1诱导大脑中动脉闭塞

Creation of a cranial window is a method that allows direct visualization of structures on the cortical surface of the brain1-3. This technique can be ...

Optimized System for Cerebral Perfusion Monitoring in the Rat Stroke Model of Intraluminal Middle Cerebral Artery Occlusion

优化系统的脑灌注监测在中风模型大鼠的大脑中动脉闭塞腔内

The translational potential of pre-clinical stroke research depends on the accuracy of experimental modeling. Cerebral perfusion monitoring in animal ...

Permanent Cerebral Vessel Occlusion via Double Ligature and Transection

Permanent Cerebral Vessel Occlusion via Double Ligature and Transection

永久性脑血管闭塞<em&gt;通过</em&gt;双人结扎横断

Stroke is a leading cause of death, disability, and socioeconomic loss worldwide. The majority of all strokes result from an interruption in blood flow ...

Modeling Stroke in Mice: Permanent Coagulation of the Distal Middle Cerebral Artery

造型中风小鼠：在远端大脑中动脉永久性混凝

Stroke is the third most common cause of death and a main cause of acquired adult disability in developed countries. Only very limited therapeutical ...

Embolic Middle Cerebral Artery Occlusion (MCAO) for Ischemic Stroke with Homologous Blood Clots in Rats

Embolic Middle Cerebral Artery Occlusion MCAO for Ischemic Stroke with Homologous Blood Clots in Rats

Clinically, thrombolytic therapy with use of recombinant tissue plasminogen activator (tPA) remains the most effective treatment for acute ischemic ...

Permanent Ligation of the Left Anterior Descending Coronary Artery in Mice: A Model of Post-myocardial Infarction Remodelling and Heart Failure

左冠状动脉前降支的小鼠结扎：对心肌梗死后重塑和心衰模型

Heart failure is a syndrome in which the heart fails to pump blood at a rate commensurate with cellular oxygen requirements at rest or during stress. It ...

Performing Permanent Distal Middle Cerebral with Common Carotid Artery Occlusion in Aged Rats to Study Cortical Ischemia with Sustained Disability

执行永久远端大脑中与老年大鼠颈总动脉闭塞研究皮层缺血具有持续残疾

Stroke typically occurs in elderly people with a range of comorbidities including carotid (or other arterial) atherosclerosis, high blood pressure, ...

Surgical Approach for Middle Cerebral Artery Occlusion and Reperfusion Induced Stroke in Mice

对大脑中动脉闭塞再灌注损伤诱发中风小鼠的手术方法

Stroke is a leading cause of death worldwide and continues to be one of the major causes of long-term adult disabilities. About 87% of strokes are ...