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DOI :
10.3791/3041-v
•
12:38 min
August 9th, 2011
Chapters
0:05
Title
2:04
Frozen-hydrated Electron Microscopy (EM) Specimen Preparation
3:22
Cryo-EM of HIV-1 Capsid Protein Tubular Assemblies
5:26
Helical Indexing
7:13
Three-dimensional Reconstruction
10:42
Representative Results: HIV-1 Capsid Assemblies Structure Determination
12:16
Conclusion
本文介绍了一种方法来获得一个三维(3D)螺旋组装使用低温电子显微镜的分子结构。在这个协议中,我们使用的HIV - 1衣壳集会,说明详细的三维重建过程,实现了由迭代螺旋空间重构方法的密度图。
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