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DOI :
10.3791/3041-v
•
12:38 min
August 9th, 2011
Chapters
0:05
Title
2:04
Frozen-hydrated Electron Microscopy (EM) Specimen Preparation
3:22
Cryo-EM of HIV-1 Capsid Protein Tubular Assemblies
5:26
Helical Indexing
7:13
Three-dimensional Reconstruction
10:42
Representative Results: HIV-1 Capsid Assemblies Structure Determination
12:16
Conclusion
この記事では、低温電子顕微鏡を用いて螺旋状に組み立てられた分子の3次元(3D)構造を取得する方法を説明します。このプロトコルでは、我々は反復ヘリカル実空間再構成法による密度地図を達成するための詳細な3次元再構成の手順を説明するために、HIV - 1キャプシドアセンブリを使用してください。
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