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DOI :
10.3791/3059-v
September 21st, 2011
Chapters
0:05
Title
1:37
Preparation of Cell Cytosol Containing Functional Glucocorticoid Receptor
3:04
Immunoabsorption of GR from the Cytosol and Dissociation of hsp90
5:16
Reconstitution of GR-hsp90 Heterocomplex using Molecular Chaperones, Cofactors, and ATP
6:17
Analysis of Reconstituted Protein Complexes and Ligand Binding Activity
8:26
Representative GR-hsp90 Heterocomplex Formation and Steroid Binding Activity
10:04
Conclusion
체외에서 방법을 설명합니다. 방법은 소금 조직 및 단백질 복잡한 reconstitution 다음 재조합 GR의 immunoadsorption을 활용합니다. 잠재적인 방법을 응용 프로그램이 같은 cofactors와 버퍼 조건의 중요성이 논의됩니다.
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