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DOI :
10.3791/3061-v
•
8:30 min
July 18th, 2011
Chapters
0:05
Title
1:12
Preparing DNA and Protein Samples and Mounting AFM Probe
2:43
Preparing the AFM Prior to Sample Imaging
4:33
Imaging Mica Sample Surface and Biomolecules of Interest
6:25
Representative AFM Images
7:48
Conclusion
是描述一个轻敲模式原子力显微镜(AFM)的可视化的质粒DNA,细胞质的蛋白质和DNA -蛋白质复合物的方法。该方法包括准备样品的原子力显微镜成像,生化操纵的替代方法。在近生理缓冲条件下观察DNA含有特定的蛋白相互作用的区域。
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