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DOI :
10.3791/3065-v
•
6:55 min
July 11th, 2011
Chapters
0:05
Title
1:00
Culture of Human Cord Blood-derived Endothelial Colony-forming Cells (ECFCs) and Bone-marrow Derived Mesenchymal Stem Cells (MSCs)
2:51
Resuspension of Cells in Collagen/Fibronectin/Fibrinogen Solution (Day 0)
4:05
Injection into Immunodeficient Nude Mouse (Day 0)
4:51
Harvesting the Implants (Day 7) and Evaluation
5:50
Cell Morphology
6:38
Conclusion
在这里,我们描述了一种方法来提供人脐血源性内皮细胞集落形成细胞(ECFCs)和骨髓来源的间质干细胞(MSCS),胶原蛋白/纤维连接蛋白凝胶中嵌入皮下到免疫缺陷小鼠,。这种细胞/凝胶相结合,产生一个人的血管网,用鼠标血管连接。
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