Name: intraspinal cell transplantation for targeting cervical ventral horn in amyotrophic lateral sclerosis and traumatic spinal cord injury
Uploaded: 2011-09-18T14:00:00
Description: Watch this Scientific Journal Video about Intraspinal Cell Transplantation for Targeting Cervical Ventral Horn in Amyotrophic Lateral Sclerosis and Traumatic Spinal Cord Injury at JoVE.com

我想感谢：莱波雷的所有成员，Maragakis和罗斯坦有益的讨论实验室;美国和克雷格H.尼尔森基金会拨款瘫痪退伍军人。

方法 1。细胞制备作为一个例子，我们将介绍的程序编制胶质祖细胞移植，因为我们的经验与此类型的细胞12。然而，该协议的细节，包括中型和胰蛋白酶的使用，例如，将依赖于特定的细胞用于移植的类型。 <ol><li>所有的解决方案前温37.0 ° C的水洗澡。 </li><li>的HBSS冲洗烧瓶2X。添加5.0 0.05％胰蛋白酶/ EDTA mL/T-75烧瓶。在37.0 ° C培养箱中孵育3分钟烧...

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研究涉及的SOD1 G93A小鼠和大鼠，年龄和性别匹配的一组内的动物，和分发不同群体的同一窝内的动物。最好是使用ALS和SCI模型相同性别的所有动物，因为疾病的进程可能男性和女性之间有所不同，但是，它也可能是有用的男女双方有足够的动物，以检测出可能特定性别的影响，这一现象已报道G93A SOD1的老鼠13和G93A SOD1的老鼠14,15。考虑细胞移植到动物的年?...

<table border="1"><tbody><tr><td> 试剂名称 </td><td> 公司 </td><td> 目录编号 </td></tr><tr><td>的HBSS </td><td> Gibco公司</td><td> 14170 </td></tr><tr><td> 0.05％胰蛋白酶</td><td> Gibco公司</td><td> 25300 </td></tr><tr><td>大豆胰蛋白酶抑制剂（可选） </td><td>西格玛</td><td> T - 6522 </td></tr><tr><td> Acepromazine马来酸（0.7毫克/公斤） </td><td>发酵动物健康</td><td></td></tr><tr><td>氯胺酮（95毫克/公斤） </td><td>道奇堡动物健康</td><td></td></tr><tr><td>甲苯噻嗪（10毫克/公斤） </td><td>拜耳</td><td></td></tr><tr><td> ＃11羽手术刀片</td><td>电子显微镜科学</td><td> 72044-11 </td></tr><tr><td>棉签涂药（6英寸） </td><td>费舍尔</td><td> 23-400-101 </td></tr><tr><td>鼠有齿镊</td><td>精细科学的工具</td><td>鼠：11023-15; 鼠标：11042-08 </td></tr><tr><td>中型弹簧剪刀</td><td>精细科学的工具</td><td> 15012-12 </td></tr><tr><td>微型弹簧剪刀</td><td>精细科学的工具</td><td> 15000-10 </td></tr><tr><td>咬骨钳</td><td>精细科学的工具</td><td>鼠：16121-14; 鼠标：16221-14 </td></tr><tr><td> Microknife </td><td>精细科学的工具</td><td> 10056-12 </td></tr><tr><td>持针</td><td>精细科学的工具</td><td> 12502-14 </td></tr><tr><td>缝合：4-0 </td><td>薇乔</td><td> S - 183 </td></tr><tr><td>主食：9毫米</td><td> Autoclip </td><td> 427631 </td></tr><tr><td>订书机：9毫米（＃203-1000反射） </td><td>世界精密仪器</td><td> 5000344 </td></tr><tr><td>暖水泵（T /泵） </td><td> Gaymar </td><td> P / ñ 07999-000 </td></tr><tr><td>环孢素A：250.0 mg/5.0毫升安瓿</td><td>诺华/ Sandimmune </td><td> NDC 0078-0109-01 </td></tr><tr><td> FK - 506 </td><td>立法会实验室</td><td> F - 4900 </td></tr><tr><td>雷帕霉素</td><td>立法会实验室</td><td> R - 5000 </td></tr><tr><td>注射器</td><td>世界精密仪器</td><td> UMP2 </td></tr><tr><td>微型4 Microsyringe泵控制器</td><td>世界精密仪器</td><td> UMC4 </td></tr><tr><td>微操作机器人</td><td>世界精密仪器</td><td>风筝 - R </td></tr><tr><td> 10.0微升汉密尔顿注射器</td><td>汉密尔顿</td><td> 80030 </td></tr><tr><td>汉密尔顿针：33号，45 °斜角，1英寸</td><td>汉密尔顿</td><td> 7803-05 </td></tr><tr><td>玻璃20.0μLmicrocapillary移液器（可选） </td><td>金布尔</td><td> 71900-20 </td></tr></tbody></table>

intraspinal cell transplantation for targeting cervical ventral horn in amyotrophic lateral sclerosis and traumatic spinal cord injury

呼吸妥协由于膈运动神经元的损失是一个衰弱的后果了大量人力外伤性脊髓损伤（SCI）的例1的比例，是与运动神经元症，肌萎缩硬化症（ALS）2支管患者死亡的最终原因。 ALS是一种破坏性的神经系统疾病的特点是较快的上下运动神经元的变性。屈服于平均2-5年的疾病的患者最终因呼吸麻痹由于膈运动神经元膜片3 innnervation损失的诊断。绝大多数情况下是零星的，而10％的家族形式。大约二十％的家族性病例是在21号染色体 4铜/锌超氧化物歧化酶1（SOD1）基因点突变。转基因小鼠4,5和6大鼠携带突变人类的SOD1基因（G93A，G37R，G86R，G85R）已经产生，并且，尽管存在着其他的动物模型运动神经元的损失，目前该疾病的最高度使用的模型。 脊髓损伤（SCI）是一个异构的条件，造成身体创伤，脊髓功能的结果，根据不同的类型，位置和严重程度的伤害 7 。然而，大约一半人类脊髓损伤病例的影响颈椎的地区，导致呼吸功能障碍衰弱由于膈运动神经元的损失和伤害的降bulbospinal呼吸轴突1。已开发的脊髓损伤动物模型，最常用的和临床相关挫伤8。 各种类的神经前体细胞（NPC的）移植是治疗外伤性中枢神经系统损伤和神经退行性疾病，包括ALS和SCI的一种很有前途的治疗策略，因为更换丢失或功能失调性中枢神经系统的细胞类型，提供神经保护作用的能力，并提供基因因素9利益。 ALS和SCI的动物模型，可以模拟许多这些疾病的临床相关的各个方面，包括膈运动神经元的损失和由此产生的呼吸妥协10,11。为了评估全国人民代表大会为基础的战略，在ALS和SCI这些动物模型的呼吸功能的疗效，必须专门针对蜂窝干预含有治疗膈运动神经元等有关目标的地区。我们提供一个详细的协议为多节段椎管内的NPC移植到颈椎脊髓腹侧灰质神经退行性型号如SOD1的G93A小鼠和大鼠，以及脊髓损伤大鼠和小鼠11。

Watch this Scientific Journal Video about Intraspinal Cell Transplantation for Targeting Cervical Ventral Horn in Amyotrophic Lateral Sclerosis and Traumatic Spinal Cord Injury at JoVE.com

Intraspinal Cell Transplantation for Targeting Cervical Ventral Horn in Amyotrophic Lateral Sclerosis and Traumatic Spinal Cord Injury

神经前体移植是一种很有前途的战略，保护和/或更换丢失/功能失调颈椎膈运动神经元在脊髓损伤（SCI）和运动神经元症，肌萎缩边音硬化症（ALS）。我们提供了一个细胞传递到脊髓型颈椎病ALS和SCI的啮齿类动物模型腹角的协议。

椎管内针对肌萎缩性脊髓侧索硬化症和外伤性脊髓损伤颈腹角细胞移植

Respiratory compromise due to phrenic motor neuron loss is a debilitating consequence of a large proportion of human traumatic spinal cord injury (SCI) ...

Research

JoVE Journal

Medicine

A Protocol for Comprehensive Assessment of Bulbar Dysfunction in Amyotrophic Lateral Sclerosis (ALS)

A Protocol for Comprehensive Assessment of Bulbar Dysfunction in Amyotrophic Lateral Sclerosis ALS

延髓功能障碍肌萎缩性脊髓侧索硬化症（ALS）的综合评估议定书

Improved methods for assessing bulbar impairment are necessary for expediting diagnosis of bulbar dysfunction in ALS, for predicting disease progression ...

科研

医学

Acute and Chronic Tactile Sensory Testing after Spinal Cord Injury in Rats

在大鼠脊髓损伤后的急性和慢性触觉测试

Spinal cord injury (SCI) impairs sensory systems causing allodynia1-8. To identify cellular and molecular causes of allodynia, sensitive and valid sensory ...

A Contusive Model of Unilateral Cervical Spinal Cord Injury Using the Infinite Horizon Impactor

单侧颈脊髓损伤的挫伤模型，利用无限的地平线冲击

While the majority of human spinal cord injuries occur in the cervical spinal cord, the vast majority of laboratory research employs animal models of ...

Clinical Testing and Spinal Cord Removal in a Mouse Model for Amyotrophic Lateral Sclerosis (ALS)

Clinical Testing and Spinal Cord Removal in a Mouse Model for Amyotrophic Lateral Sclerosis ALS

临床试验和脊髓去除在肌萎缩性侧索硬化症的小鼠模型（ALS）

Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disorder resulting in progressive degeneration of motoneurons. Peak of onset is around 60 ...

Controlled Cervical Laceration Injury in Mice

控制宫颈裂伤损伤小鼠

Use of genetically modified mice enhances our  understanding of molecular mechanisms underlying several neurological disorders  such as a spinal cord ...

A Murine Model of Cervical Spinal Cord Injury to Study Post-lesional Respiratory Neuroplasticity

颈脊髓损伤的小鼠模型研究后皮损呼吸神经可塑性

A cervical spinal cord injury induces permanent paralysis, and often leads to respiratory distress. To date, no efficient therapeutics have been developed ...

Controlled Cortical Impact Model for Traumatic Brain Injury

控制皮层影响模型创伤性脑损伤

Every year over a million Americans suffer a traumatic brain injury (TBI).  Combined with the incidence of TBIs worldwide, the physical, emotional, ...

A Radio-telemetric System to Monitor Cardiovascular Function in Rats with Spinal Cord Transection and Embryonic Neural Stem Cell Grafts

无线电遥测系统，以监测大鼠脊髓横断和胚胎神经干细胞移植在心血管功能

High thoracic or cervical spinal cord injury (SCI) can lead to cardiovascular dysfunction. To monitor cardiovascular parameters, we implanted a catheter ...

Synergetic Use of Neural Precursor Cells and Self-assembling Peptides in Experimental Cervical Spinal Cord Injury

在实验颈脊髓损伤协同使用神经前体细胞，并自组装肽的

Spinal cord injuries (SCI) cause serious neurological impairment and psychological, economic, and social consequences for patients and their families. ...

Neural Stem Cell Transplantation in Experimental Contusive Model of Spinal Cord Injury

在脊髓损伤的实验挫伤型号神经干细胞移植

Spinal cord injury is a devastating clinical condition, characterized by a complex of neurological dysfunctions. Animal models of spinal cord injury can ...