Name: modified technique for coronary artery ligation in mice
Uploaded: 2013-03-29T12:00:00
Description: Watch this Scientific Journal Video about Modified Technique for Coronary Artery Ligation in Mice at JoVE.com

1。协议文本<ol><li>在进行手术前，麻醉一个雄性小鼠（C57BL / 6），6个星期以上的老年人吸入2-5％的异氟醚。执行一个脚趾捏确认该动物是充分麻醉。 </li><li>无意识的鼠标被放置在一个温暖的垫在它的后面，以维持一个恒定的温度为37℃。剃了头的左侧胸部心前区。 </li><li>鼠标固定到插管装置与异氟醚填充室。将0.2内直径管到鼠标的气管中，通过口，然后在气管插管鼠标上的无菌手术区（37°Ç?...

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本报告中的外科手术的LCA结扎作为证明是一个可靠和可重复的方法诱导实验性心肌梗死的小鼠，6。从事通常结扎LCA根据本协议引起心肌缺血，左心室3％〜40％。这会引起显着的前心脏表面心电图ST段可见面色苍白。由于的大型心肌梗死的，死亡率可高达30％8，9。应注意事项，各动物品系，心肌梗死面积和心肌梗死后心肌死亡可能是不同的。该方法具有几个优点。皮肤切口...

modified technique for coronary artery ligation in mice

心肌梗死（MI）是在人类1-3死亡率的最重要的原因之一。为了提高我们需要更好地了解心肌缺血的病理生理与MI患者的发病率和死亡率。这方面的知识可能是有价值的，创新的心血管治疗4定义新的治疗靶点。在小鼠实验性心肌梗死模型是一个日益流行的小动物模型的临床前研究中，MI引起的永久或临时结扎左冠状动脉（LCA）5。在这段视频中，我们描述了如何诱导小鼠实验性心肌梗死的一步一步的方法。 动物是先用2％异氟烷麻醉。无意识的鼠标，然后插管，连接呼吸机进行人工通风。左胸口被刮了胡子，在皮肤上作1.5厘米的切口，沿腋中线。左胸大肌是直言dissoc的的iated直到肋露出。肌肉层被拉到一边，眼睑拉钩固定。这些制剂后，进行左侧开胸之间的第三和第四肋，为了可视化的心脏的前表面及左肺。近段的LCA动脉的连接与一个7-0 ethilon的缝合通常诱导的心肌梗死面积〜40％的左心室。结束时，胸部被关闭和动物接受术后的镇痛（Temgesic，0.3 mg/50毫升，IP）。动物被关在一个温暖的笼子里，直到自然恢复。

<html><table border="1" ><tbody><tr><td> 仪器 </td><td> 功能说明 </td></tr><tr><td>钝尖剪刀</td><td>用于切割皮肤，肋间胸部肌肉，缝合</td></tr><tr><td>眼睛牵引器</td><td>用于揭露外科手术视图</td></tr><tr><td>钝尖镊子</td><td>用来掌握皮肤和肌肉</td></tr><tr><td>持针器</td><td>要保持针</td></tr><tr><td> 5-0缝合</td><td>用于缝合皮肤</td></tr><tr><td> 6-0缝合</td><td>用于缝合胸部肌肉</td></tr><tr><td> 7-0缝合</td><td>冠状动脉结扎<...

Watch this Scientific Journal Video about Modified Technique for Coronary Artery Ligation in Mice at JoVE.com

Modified Technique for Coronary Artery Ligation in Mice

用于诱导小鼠实验性心肌梗死的外科手术开始之间的第三和第四肋左侧开胸，为了可视化的心脏的前表面及左肺。左冠状动脉结扎后，胸部被关闭，鼠标被允许自行恢复。

改性技术在小鼠冠状动脉结扎

Myocardial infarction (MI) is one of the most important causes of mortality in humans1-3. In order to improve morbidity and mortality in patients with MI ...

Research

JoVE Journal

Medicine

Use of a Hanging Weight System for Coronary Artery Occlusion in Mice

悬重系统的使用在小鼠冠状动脉闭塞

Murine studies of acute injury are an area of intense investigation, as knockout mice for different genes are becoming increasingly available 1-38. ...

科研

医学

Coronary Artery Ligation and Intramyocardial Injection in a Murine Model of Infarction

结扎冠状动脉内注射在小鼠模型梗死

Mouse models are a valuable tool for studying acute injury and chronic remodeling of the myocardium in vivo.  With the advent of genetic modifications to ...

The Application Of Permanent Middle Cerebral Artery Ligation in the Mouse

在小鼠永久性大脑中动脉结扎术中的应用

Focal cerebral ischemia is among the most common type of stroke seen in patients. Due to the clinical significance there has been a prolonged effort to ...

Carotid Artery Infusions for Pharmacokinetic and Pharmacodynamic Analysis of Taxanes in Mice

颈动脉输液的紫杉烷类化合物在小鼠体内的药代动力学和药效学分析

When proposing the use of a drug, drug combination, or drug delivery into a novel system, one must assess the pharmacokinetics of the drug in the study ...

Permanent Ligation of the Left Anterior Descending Coronary Artery in Mice: A Model of Post-myocardial Infarction Remodelling and Heart Failure

左冠状动脉前降支的小鼠结扎：对心肌梗死后重塑和心衰模型

Heart failure is a syndrome in which the heart fails to pump blood at a rate commensurate with cellular oxygen requirements at rest or during stress. It ...

Ultrasound Based Assessment of Coronary Artery Flow and Coronary Flow Reserve Using the Pressure Overload Model in Mice

冠脉流量和冠状动脉血流储备用小鼠压力超负荷基于模型的超声评估

Transthoracic Doppler echocardiography (TTDE) is a clinically useful, noninvasive tool for studying coronary artery flow velocity and coronary flow ...

Murine Left Anterior Descending (LAD) Coronary Artery Ligation: An Improved and Simplified Model for Myocardial Infarction

Murine Left Anterior Descending LAD Coronary Artery Ligation: An Improved and Simplified Model for Myocardial Infarction

鼠左前降支（LAD）冠状动脉结扎：改进和简化模型的心肌梗死

Ischemic heart disease (IHD), or acute coronary syndrome (ACS), is one of the leading causes of death in the United States. IHD is characterized by ...

Direct Re-implantation of Left Coronary Artery into the Aorta in Adults with Anomalous Origin of Left Coronary Artery from the Pulmonary Artery (ALCAPA)

Direct Re-implantation of Left Coronary Artery into the Aorta in Adults with Anomalous Origin of Left Coronary Artery from the Pulmonary Artery ALCAPA

左冠状动脉直接再注入与从肺动脉左冠状动脉起源异常成人主动脉（ALCAPA）

Anomalous origin of the left coronary artery from the pulmonary artery (ALCAPA) is a rare congenital anomaly which is one of leading causes of myocardial ...

Identifying Coronary Artery Calcification on Non-gated Computed Tomography Scans

非门控断层扫描的冠状动脉钙化识别

Coronary artery calcification (CAC) provides an objective measure of coronary artery disease and can readily be identified on non-gated computed ...

Murine Myocardial Infarction Model using Permanent Ligation of Left Anterior Descending Coronary Artery

使用左前部降冠动脉的永久结扎的月心肌梗死模型

Myocardial infarction (MI) and acute coronary diseases are among the most prominent causes of death in population with western lifestyle. The murine ...