Name: modified technique for coronary artery ligation in mice
Uploaded: 2013-03-29T12:00:00
Description: Watch this Scientific Journal Video about Modified Technique for Coronary Artery Ligation in Mice at JoVE.com

1. Protokol Text <ol><li> Før du går til kirurgi, bedøve en mandlig mus (C57BL / 6), som er 6 uger gamle eller ældre ved indånding på 2-5% isofluran. Udfør en tå knibe for at bekræfte, at dyret er tilstrækkeligt bedøvet. </li><li> Den ubevidste mus anbringes på ryggen på en varm pad at opretholde en konstant temperatur på 37 ° C. Den precordial brystet på venstre side er barberet. </li><li> Fastgør musen til en intubation enhed med en isofluran-fyldt kammer. Sæt et rør på 0,2 indvendig diameter i m...

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	<li>Tabrizchi, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Beta-blocker+therapy+after+acute+myocardial+infarction.">Beta-blocker therapy after acute myocardial infarction.</a> Expert Rev. Cardiovasc. Ther. 11, 293-296 (2013).</li><li>Pell, S., Fayerweather, W. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Trends+in+the+incidence+of+myocardial+infarction+and+in+associated+mortality+and+morbidity+in+a+large+employed+population,+1957-1983.">Trends in the incidence of myocardial infarction and in associated mortality and morbidity in a large employed population, 1957-1983.</a> N. Engl. J. 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Den kirurgiske procedure for LCA-ligering som det fremgår af denne rapport er en pålidelig og reproducerbar metode til induktion af eksperimentel MI i mus 3, 6. Ligeringen af LCA overensstemmelse med denne protokol inducerer myocardial iskæmi indgreb typisk ~ 40% af venstre ventrikel 3. Dette inducerer signifikante EKG ændringer i ST-segmentet 7 og synlige bleghed på den forreste overflade af hjertet. På grund af den store myokardieinfarkt, kan dødeligheden være op til 30% 8...

modified technique for coronary artery ligation in mice

Myokardieinfarkt (MI) er en af de vigtigste dødsårsager hos mennesker 1-3. For at forbedre morbiditet og mortalitet hos patienter med MI vi har brug for bedre viden om patofysiologien af ​​myokardieiskæmi. Denne viden kan være værdifuld til at definere nye terapeutiske mål for innovative behandling af kardiovaskulære sygdomme 4. Eksperimentel MI model i mus er en stadig mere populær lille dyremodel i præklinisk forskning, hvor MI induceres ved hjælp af permanent eller midlertidig ligering af venstre koronararterie (LCA) 5. I denne video beskriver vi trin-for-trin metode til at fremkalde eksperimentel MI hos mus. Dyret først bedøvet med 2% isofluran. Den bevidstløse mus derefter intuberet og forbundet med en ventilator til kunstig ventilation. Venstre bryst barberes og 1,5 cm indsnit langs mid-axillær linie er lavet i huden. Den venstre pectoralis store muskler er ligeud dissociated indtil ribberne udsættes. De muskellag er trukket til side og fastgøres med en øjenlåg-retractor. Efter disse præparater, er venstre torakotomi udføres mellem tredje og fjerde ribben for at visualisere den forreste overflade af hjertet og venstre lunge. Det proximale segment af LCA arterie ligeres derefter med et 7-0 ethilon sutur, som typisk inducerer en infarktstørrelse ~ 40% af venstre ventrikel. Ved afslutningen er brystet lukket, og dyrene får postoperativ analgesi (Temgesic, 0,3 mg/50 ml, ip). Dyrene holdes i en varm bur indtil spontan helbredelse.

<html><table border="1" ><tbody><tr><td> Instrument </td><td> Funktionsbeskrivelse </td></tr><tr><td> Blunt-tip saks </td><td> Anvendes til at skære huden, intercostal bryst muskel, og suturer </td></tr><tr><td> Eye retraktor </td><td> Anvendt til at belyse kirurgisk view </td></tr><tr><td> Blunt-tip pincet </td><td> Anvendes til at gribe hud og muskel </td></tr><tr><td> Nåleholder </td><td> At holde en nål </td></tr><tr><td> 5-0 sutur </td><td> Anvendes til at suturere huden </td></tr><tr><td> 6-0 sutur </t...

Watch this Scientific Journal Video about Modified Technique for Coronary Artery Ligation in Mice at JoVE.com

Modified Technique for Coronary Artery Ligation in Mice

Den kirurgiske procedure anvendes til at inducere eksperimentel myokardieinfarkt hos mus begynder med venstre torakotomi mellem den tredje og den fjerde ribben for at visualisere den forreste overflade af hjertet og venstre lunge. Den venstre koronararterie ligeres, er brystet lukkes og musen får lov til at komme sig spontant.

Modificeret Teknik for Koronararterie Ligering i mus

Myocardial infarction (MI) is one of the most important causes of mortality in humans1-3. In order to improve morbidity and mortality in patients with MI ...

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Medicine

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The Application Of Permanent Middle Cerebral Artery Ligation in the Mouse

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Carotid Artery Infusions for Pharmacokinetic and Pharmacodynamic Analysis of Taxanes in Mice

Halspulsåren infusioner farmakokinetisk og farmakodynamisk analyse af Taxaner i mus

When proposing the use of a drug, drug combination, or drug delivery into a novel system, one must assess the pharmacokinetics of the drug in the study ...

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Heart failure is a syndrome in which the heart fails to pump blood at a rate commensurate with cellular oxygen requirements at rest or during stress. It ...

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Ultralyd funderet vurdering af koronararterie Flow og koronar Flow Reserve Brug af trykoverbelastning Model i mus

Transthoracic Doppler echocardiography (TTDE) is a clinically useful, noninvasive tool for studying coronary artery flow velocity and coronary flow ...

Murine Left Anterior Descending (LAD) Coronary Artery Ligation: An Improved and Simplified Model for Myocardial Infarction

Murine Left Anterior Descending LAD Coronary Artery Ligation: An Improved and Simplified Model for Myocardial Infarction

Muse Venstre Anterior Faldende (LAD) kranspulsåreombinding: En forbedret og forenklet model for myokardieinfarkt

Ischemic heart disease (IHD), or acute coronary syndrome (ACS), is one of the leading causes of death in the United States. IHD is characterized by ...

Direct Re-implantation of Left Coronary Artery into the Aorta in Adults with Anomalous Origin of Left Coronary Artery from the Pulmonary Artery (ALCAPA)

Direct Re-implantation of Left Coronary Artery into the Aorta in Adults with Anomalous Origin of Left Coronary Artery from the Pulmonary Artery ALCAPA

Direkte Re-implantation af venstre koronararterie i aorta i Voksne med Anomalous Oprindelse af venstre koronararterie fra lungepulsåren (ALCAPA)

Anomalous origin of the left coronary artery from the pulmonary artery (ALCAPA) is a rare congenital anomaly which is one of leading causes of myocardial ...

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At identificere koronararterie forkalkning på beregnet ikke-gated tomografi scanninger

Coronary artery calcification (CAC) provides an objective measure of coronary artery disease and can readily be identified on non-gated computed ...

Murine Myocardial Infarction Model using Permanent Ligation of Left Anterior Descending Coronary Artery

Murine myokardieinfarkt model ved hjælp af permanent ligation af venstre anterior faldende koronararterie

Myocardial infarction (MI) and acute coronary diseases are among the most prominent causes of death in population with western lifestyle. The murine ...