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DOI :
10.3791/3096-v
•
7:08 min
September 23rd, 2011
Chapters
0:05
Title
1:25
Crosslinking of Plant Material
2:25
Isolation of Chromatin
4:18
Immunoprecipitation
5:26
Locus-specific DNA Quantitation
5:55
Benzothiadiazole (BTH) Induced Histone Acetylation on the PR2 Promoter in Arabidopsis
6:20
Conclusion
特定の植物遺伝子上にヒストン修飾を検出するために信頼性の高い有用なアプローチが説明されています。アプローチは、クロマチン免疫沈降(ChIP)とリアルタイム定量PCRを兼ね備えています。それは、多様な生理学的プロセスにおいて役割を持つ特定の遺伝子のヒストン修飾の検出を可能にする。
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