Name: high-resolution endocardial and epicardial optical mapping in a sheep model of stretch-induced atrial fibrillation
Uploaded: 2011-07-29T14:00:00
Description: Watch this Scientific Journal Video about High-Resolution Endocardial and Epicardial Optical Mapping in a Sheep Model of Stretch-Induced Atrial Fibrillation at JoVE.com

部分支持NHLBI资助HL039707 P01 - P01 - HL087226和Leducq基金会（JJ和OB），由西班牙社会的心脏病奖学金基金会，佩德罗巴里DE LA的Maza和基金会阿方马丁Escudero（DFR）联邦法国， Cardiologie（RPM），心脏节律协会奖学金奖，日本心脏基金会的奖学金/电学（我的）日本社会。

1。摘取心脏Langendorff灌流羊重达35-40公斤，用4-6毫克/公斤异丙酚和60-100毫克/公斤戊巴比妥钠麻醉。心被删除通过开胸手术和循环含氧（95％O2，5％CO2）台氏在一个恒定流速为240-270毫升/分钟，pH值7.4和35.5-37.5 ° C。的解决方案连接到的Langendorff灌注系统台氏（毫米）的组成是：氯化钠，4.0氯化钾，氯化镁，氯化钙1.8，碳酸氢钠24，磷酸二氢钠1.2，葡萄糖5.6 130，和白蛋白0.04 g / L时B...

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中分离出来的羊心的拉伸引起的急性房颤的特点类似于人类的阵发性房颤的一些属性。 1中的左到右的DF梯度的存在在急性羊心内心房压力的增加使维护长期的时间，观察患者心房扩张的风险较高的AF自动对焦。羊心房也有类似的人体电生理研究注册的 16因此，了解的机制，维持在此急性模型的自动对焦，可改善目前在人类的阵发性房颤的治疗策略。一些当前方法的局限性是：第一...

<table border="1"><tbody><tr><td> 材料名称 </td><td> 公司 </td><td> 目录编号 </td></tr><tr><td> 安乐死 </td><td></td><td></td></tr><tr><td>肝素</td><td>西格玛</td><td> H3393 </td></tr><tr><td>异丙酚</td><td>雅培</td><td> 5206-04-03 </td></tr><tr><td>戊巴比妥</td><td> Lundbeck公司公司</td><td> NDC 67386-501-55 </td></tr><tr><td>介绍人18号</td><td> Terumo公司</td><td> SS * FF1832 </td></tr><tr><td>翻边气管导管（9毫米） </td><td>肛门指诊兽医</td><td> ＃9440 </td></tr><tr><td>光纤喉镜案例</td><td>肛门指诊兽医</td><td> ＃991 </td></tr><tr><td>光纤刀片</td><td>肛门指诊兽医</td><td> ＃984 </td></tr><tr><td>操作剪刀</td><td>肛门指诊兽医</td><td> ＃9702＃1944 </td></tr><tr><td>手术刀手柄＃3固体4“ </td><td> Roboz外科仪器有限责任公司</td><td> RS - 9843 </td></tr><tr><td>无菌手术刀片</td><td> Roboz外科仪器有限责任公司</td><td> RS - 9801 - 10 </td></tr><tr><td>通风袋</td><td> Westmed </td><td> 562013 </td></tr><tr><td>模拟人生剪刀弯夏普/钝</td><td> Roboz外科仪器有限责任公司</td><td> RS - 7035 </td></tr><tr><td>组织钳（× 2） </td><td>肛门指诊兽医</td><td> ＃1895年</td></tr><tr><td>坎特罗威茨胸钳，11“ </td><td>生物医学研究仪器公司</td><td> 34-1980 </td></tr><tr><td> Finochietto大胸部吊具</td><td>卡普外科仪器公司</td><td> KS - 7301 </td></tr><tr><td>开胸手术剪</td><td> Rostfrei索林根</td><td></td></tr><tr><td>塑料托盘</td><td> NALGENE </td><td>菲舍尔</td></tr><tr><td> 光的映射 </td><td></td><td></td></tr><tr><td>波恩剪刀（× 2） </td><td> Roboz手术器械有限责任公司</td><td> RS - 5840SC </td></tr><tr><td>手术丝</td><td>菲舍尔</td><td> 50-900-04214 </td></tr><tr><td>微解剖钳</td><td> Roboz手术器械有限责任公司</td><td> RS - 5130 </td></tr><tr><td> Tetrapolar电极导管（TORQ）（× 4） </td><td>美敦力公司</td><td> 05580SP </td></tr><tr><td>数字传感器。 BIOPAC系统传感器</td><td> BIOPAC系统，公司</td><td> RX104A </td></tr><tr><td> BIOPAC系统放大器</td><td> BIOPAC系统，公司</td><td> DA - 100C </td></tr><tr><td> DI - 4 - ANEPPS </td><td> Sigma - Aldrich公司，圣</td><td> D8604 - 5毫克</td></tr><tr><td> Blebbistatin </td><td>恩佐生命科学国际公司</td><td> BML - E1315 - 0025 </td></tr><tr><td> LittleJoe CCD摄像机（× 2） </td><td> SciMeasure分析系统，公司</td><td></td></tr><tr><td>双通道刚性内窥镜</td><td>珠峰VIT公司</td><td> R10 - 25 - 0 - 90 </td></tr><tr><td>灌注泵（× 2） </td><td>科尔帕默</td><td> GK - 77920 - 30 </td></tr><tr><td>温度探头</td><td>科尔帕默</td><td> R - 08491 - 02 </td></tr><tr><td>酸度计</td><td>菲舍尔</td><td> 01-913-806 </td></tr><tr><td>数字温度测量仪</td><td>科尔帕默</td><td> GK89000 - 10 </td></tr><tr><td>充氧过滤器</td><td>索林</td><td> 05318 </td></tr><tr><td>硅灌注管（L / S 15） </td><td> MASTERFLEX </td><td> 96410-15 </td></tr><tr><td>激光导光（× 6） </td><td>奥丽尔公司</td><td> 77536 </td></tr><tr><td>液体光导（0.2核心） </td><td> Newport公司</td><td> 77556 </td></tr><tr><td>激光发生器（1瓦）（× 1） </td><td>上海梦Lsaer Tecchnology </td><td> SDL - 532 - 1000T </td></tr><tr><td>激光发生器（5瓦）（× 1） </td><td>光谱物理激光器</td><td>轧机5sJ </td></tr></tbody></table>

high-resolution endocardial and epicardial optical mapping in a sheep model of stretch-induced atrial fibrillation

心房颤动（AF）是一个复杂的的心律失常，发病率和死亡率高 。1,2它是最常见的持续心脏节律紊乱在临床实践中，其患病率在未来几年内预计将增加3内心房压力增加和扩张早就认识到导致自动对焦，其中强调利用动物模型的相关性和拉伸研究自动对焦动态的1,4。了解底层自动对焦的机制，需要可视化的高时空分辨率的心脏电波。虽然高时空分辨率，可以实现由传统的用于人体电生理研究的常规电气映射，内心房电极的少数，可以同时使用，限制了空间分辨率和排除任何详细的追踪期间心律失常的电波。引进的光映射在90年代初启用宽领域fibrillatory活动的表征与亚毫米级的空间分辨率在动物模型5,6和快速旋转的电波模式（转子）fibrillatory来源识别活动中可能发生的心室或心房。7-9使用相结合的时间和频域的分析，光映射，它是可能的，证明在AF高频率的定期活动的离散网站，沿左，右心房之间的频率梯度。与最快转子的地区，出现频率最高的激活和驱动器整体的心律失常。10,11波这样的转子所产生的交互功能性或解剖上的障碍，在他们的道路fibrillatory传导现象。12测绘内膜表面后左心房（解放军），允许与转子频率最高的自动对焦波在该地区的动态跟踪。重要的是，解放军是腔内导管为基础的烧蚀程序是最成功的终止AF患者中，13，其中强调了学习的左心房室内的自动对焦动态的相关性的地区。在这里，我们描述了一个羊急性牵张诱导AF，它类似于人类阵发性房颤的一些特征模型。补充左心房心外膜标测与心内膜解放军测绘使用的是双通道刚性内窥镜C -安装一个CCD摄像头，它代表了最直接的方法，可视化最相关的区域的激活模式，自动对焦维护。

Watch this Scientific Journal Video about High-Resolution Endocardial and Epicardial Optical Mapping in a Sheep Model of Stretch-Induced Atrial Fibrillation at JoVE.com

High-Resolution Endocardial and Epicardial Optical Mapping in a Sheep Model of Stretch-Induced Atrial Fibrillation

这份报告提供了并发心内膜和心外膜完整的Langendorff灌注期间拉伸诱发房颤羊心左心房的电激发光的映射的方法和结果的详细说明。

高分辨率的心内膜和心外膜光学在拉伸诱发心房颤动的羊模型的映射

 Atrial fibrillation (AF) is a complex cardiac arrhythmia with high morbidity and mortality.1,2 It is the most common sustained cardiac rhythm disturbance ...

Research

JoVE Journal

Medicine

The WATCHMAN Left Atrial Appendage Closure Device for Atrial Fibrillation

守望的左心耳封闭装置心房颤动

Atrial fibrillation (AF) is the most common cardiac arrhythmia, affecting an estimated 6 million people in the United States 1. Since AF affects primarily ...

科研

医学

Catheter Ablation in Combination With Left Atrial Appendage Closure for Atrial Fibrillation

结合消融房颤左心耳封堵

Atrial fibrillation (AF) is the most common sustained cardiac arrhythmia, affecting millions of individuals worldwide 1-3. The rapid, irregular, and ...

A Rat Model of Ventricular Fibrillation and Resuscitation by Conventional Closed-chest Technique

室颤由传统的封闭胸技术大鼠模型和复苏

A rat model of electrically-induced ventricular fibrillation followed by cardiac resuscitation using a closed chest technique that incorporates the basic ...

Robotic Ablation of Atrial Fibrillation

房颤消融机器人

Background: Pulmonary vein isolation (PVI) is an established treatment for atrial fibrillation (AF). During PVI an electrical conduction block between ...

Electrophysiological Assessment of Murine Atria with High-Resolution Optical Mapping

高分辨率光学制图对小鼠心房电生理的评价

Recent genome-wide association studies targeting atrial fibrillation (AF) have indicated a strong association between the genotype and ...

High-Throughput Analysis of Optical Mapping Data Using ElectroMap

使用电子地图的光映射数据的高通量分析

Optical mapping is an established technique for high spatio-temporal resolution study of cardiac electrophysiology in multi-cellular preparations. Here we ...

Standardized Model of Ventricular Fibrillation and Advanced Cardiac Life Support in Swine

猪心室颤动和高级心脏生命支持标准化模型

Cardiopulmonary resuscitation after cardiac arrest, independent of its origin, is a regularly encountered medical emergency in hospitals as well as ...

Cooling or Warming the Esophagus to Reduce Esophageal Injury During Left Atrial Ablation in the Treatment of Atrial Fibrillation

在心房颤动治疗中，为食道冷却或加热，以减少左心房消融期间的食管损伤

Ablation of the left atrium using either radiofrequency (RF) or cryothermal energy is an effective treatment for atrial fibrillation (AF) and is the most ...

Sterile Pericarditis in Aachener Minipigs As a Model for Atrial Myopathy and Atrial Fibrillation

Aachener Minipigs的无菌性心包炎作为心房肌病和心房颤动的模型

Atrial fibrillation (AF) is the most common arrhythmia caused by structural remodeling of the atria, also called atrial myopathy. Current therapies only ...

High-Resolution Three-Dimensional Imaging of the Footpad Vasculature in a Murine Hindlimb Gangrene Model

小鼠后肢坏死模型中脚垫脉管系统的高分辨率三维成像

Peripheral arterial disease (PAD) is a significant cause of morbidity resulting from chronic exposure to atherosclerotic risk factors. Patients suffering ...