Name: high-resolution endocardial and epicardial optical mapping in a sheep model of stretch-induced atrial fibrillation
Uploaded: 2011-07-29T14:00:00
Description: Watch this Scientific Journal Video about High-Resolution Endocardial and Epicardial Optical Mapping in a Sheep Model of Stretch-Induced Atrial Fibrillation at JoVE.com

Fédération Française de NHLBI Grants P01-HL039707 ve P01-HL087226 ve Leducq Vakfı (JJ ve OB), bir İspanyol Kardiyoloji Bursu Derneği tarafından, Fundación Pedro Barrie de la Maza ve Fundación Alfonso Martín Escudero (DFR) tarafından kısmen desteklenen Cardiologie (RPM), Kalp Ritmi Derneği dostluk Ödülü, Japonya Kalp Vakfı / Electrocardiology (MY) Japon Derneği Bursu.

1. Kalp kaldırılması ve Langendorff perfüzyon Koyun 35-40 Kg ağırlığında, 4-6 mg / kg propofol ve 60-100 mg / Kg pentobarbital sodyum ile anestezi. Kalpler torakotomi ile kaldırıldı ve (% 95 O2,% 5 CO2) 240-270 ml / dk, pH 7.4 ve 35,5-37,5 ° C sabit bir akış hızında Tyrode çözümü oksijenli dolaşan bir Langendorff-perfüzyon sistemine bağlı Tyrode kompozisyonu (mM): NaCl 130, KCl 4.0, MgCl2 1, CaCl2 1.8, NaHCO3 24, NaH2PO4 1.2, Glikoz 5.6 ve Albumin 0.04 g / L Blebbistat...

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Izole koyun kalp streç indüklenen akut AF özellikleri insan paroksismal AF bazı özelliklerini andırır. Koyun kalbinde intra-atriyal basınç akut bir artış AF atriyal dilatasyon olan hastalarda gözlenen AF daha yüksek bir risk benzer uzun süre bakım sağlar. Soldan sağa DF degradelerin 1 varlığı koyun kulakçıklar insan elektrofizyolojik çalışmaların kayıt yaptırmış olanlar da benzer 16 Bu nedenle, bu akut modeli AF sürdürülmesi mekanizmasının anlaşılması, şu anda...

<table border="1"><tbody><tr><td> Malzeme Adı </td><td> Şirket </td><td> Katalog Numarası </td></tr><tr><td> Ötenazi </td><td></td><td></td></tr><tr><td> Heparin </td><td> Sigma </td><td> H3393 </td></tr><tr><td> Propofol </td><td> Abbott </td><td> 5206-04-03 </td></tr><tr><td> Pentobarbital </td><td> Lundbeck Inc. </td><td> NDC 67386-501-55 </td></tr><tr><td> Introducer 18 Gauge </td><td> Terumo </td><td> SS * FF1832 </td></tr><tr><td> Kelepçeli endotrakeal tüp (9 mm) </td><td> PRM Veteriner </td><td> # 9440 </td></tr><tr><td> Fiber Optik Laringoskop Çantası </td><td> PRM Veteriner </td><td> # 991 </td></tr><tr><td> Fiber Optik Blade </td><td> PRM Veteriner </td><td> # 984 </td></tr><tr><td> İşletim Makas </td><td> PRM Veteriner </td><td> # 9702 # 1944 </td></tr><tr><td> Neşter Kolu # 3 Solid 4 &quot; </td><td> Roboz Cerrahi Instrument Co, Inc. </td><td> RS-9843 </td></tr><tr><td> Steril bistüri Bıçaklar </td><td> Roboz Cerrahi Instrument Co, Inc. </td><td> RS-9801-10 </td></tr><tr><td> Havalandırma çanta </td><td> Westmed </td><td> 562013 </td></tr><tr><td> Künt / Sharp Sims Makas Eğri </td><td> Roboz Cerrahi Instrument Co, Inc. </td><td> RS-7035 </td></tr><tr><td> Doku Forseps (× 2) </td><td> PRM Veteriner </td><td> # 1895 </td></tr><tr><td> KANTROWITZ Göğüs Forseps, 11 &quot; </td><td> Biyomedikal Araştırma Instruments, Inc. </td><td> 34-1980 </td></tr><tr><td> Finochietto Büyük Göğüs Serpme </td><td> Kapp Cerrahi Enstrüman Inc. </td><td> KS-7301 </td></tr><tr><td> Torakotomi makaslar </td><td> Rostfrei Solingen </td><td></td></tr><tr><td> Plastik tepsisi </td><td> Nalgene </td><td> Fischer </td></tr><tr><td> Optik haritalama </td><td></td><td></td></tr><tr><td> Bonn Makas (× 2) </td><td> Roboz Cerrahi Instrument Co, Inc. </td><td> RS-5840SC </td></tr><tr><td> Cerrahi ipek </td><td> Fischer </td><td> 50-900-04214 </td></tr><tr><td> Mikro Diseksiyon Forseps </td><td> Roboz Cerrahi Instrument Co, Inc. </td><td> RS-5130 </td></tr><tr><td> Tetrapolar elektrod kateterler (Torq) (× 4) </td><td> Medtronic Inc. </td><td> 05580SP </td></tr><tr><td> Dijital sensör. BIOPAC Sistemleri transduser </td><td> BIOPAC Systems, Inc. </td><td> RX104A </td></tr><tr><td> BIOPAC Sistemleri amplifikatör </td><td> BIOPAC Systems, Inc. </td><td> DA-100C </td></tr><tr><td> Di-4-ANEPPS </td><td> Sigma-Aldrich, St. </td><td> D8604-5mg </td></tr><tr><td> Blebbistatin </td><td> Enzo Yaşam Bilimleri Uluslararası, INC. </td><td> BML-E1315-0025 </td></tr><tr><td> LittleJoe CCD video kamera (× 2) </td><td> Analitik Systems, Inc. SciMeasure </td><td></td></tr><tr><td> Çift kanal sert borescope </td><td> Everest VIT, Inc. </td><td> R10-25-0-90 </td></tr><tr><td> Perfüzyon pompaları (× 2) </td><td> Cole Parmer </td><td> GK-77.920-30 </td></tr><tr><td> Sıcaklık probu </td><td> Cole Parmer </td><td> R-08.491-02 </td></tr><tr><td> pH metre </td><td> Fischer </td><td> 01-913-806 </td></tr><tr><td> Dijital sıcaklık göstergesi </td><td> Cole Parmer </td><td> GK89000-10 </td></tr><tr><td> Oksijenatör filtreleri </td><td> Sorin </td><td> 05318 </td></tr><tr><td> Silikon perfüzyon tüpleri (L / S 15) </td><td> MASTERFLEX </td><td> 96410-15 </td></tr><tr><td> Lazer ışığı kılavuzları (× 6) </td><td> Oriel Corporation&#39;ın </td><td> 77536 </td></tr><tr><td> Sıvı ışık kılavuzu (çekirdek 0.2) </td><td> Newport Corporation&#39;ın </td><td> 77556 </td></tr><tr><td> Lazer jeneratör (1 watt) (x 1) </td><td> Şangay Rüya Lsaer Tecchnology </td><td> SDL-532-1000T </td></tr><tr><td> Lazer jeneratör (5 watt) (x 1) </td><td> Spectra Fizik Lazerler </td><td> DEĞİRMEN 5sJ </td></tr></tbody></table>

high-resolution endocardial and epicardial optical mapping in a sheep model of stretch-induced atrial fibrillation

Atriyal fibrilasyon (AF), yüksek morbidite ve mortalite ile karmaşık bir kardiyak aritmi. 1,2, klinik uygulamada görülen ve görülme sıklığı, önümüzdeki yıllarda artması beklenmektedir en sık kalıcı kalp ritmi bozukluğu. 3 Artan intra -atriyal basınç ve dilatasyonu AF, hayvan modelleri kullanarak önemi vurgular ve 1,4 AF dinamiklerini incelemek kadar uzanan uzun bir yol kabul edilmiştir. AF altında yatan mekanizmaların anlaşılması, yüksek mekansal ve zamansal çözünürlük ile kalp elektrik dalgalarının görselleştirme gerektirir. Yüksek temporal çözünürlük geleneksel insan elektrofizyolojik çalışmalarda kullanılan konvansiyonel elektrik haritalama ile elde edilebilir olsa da, aynı anda kullanılabilir intra-atriyal elektrot sayısının az uzaysal çözünürlüğü sınırlar ve aritmi esnasında elektrik dalgalarının detaylı bir izleme engellemektedir. Alt-hayvan modellerinde 5,6 milimetre mekansal çözünürlükte ve fibrillatory kaynağı olarak hızla elektriksel dalga şekilleri (rotor) iplik kimlik yol ile birlikte fibrillatory aktivitenin erken 90 etkin olduğu geniş alan karakterizasyonu optik haritalama giriş ventriküller ya da kulakçıklar oluşabilir aktivite 7-9 kombine zaman ve optik haritalama frekans alanı analizleri, sol ve sağ atrium arasındaki frekans gradyanlar ile birlikte AF ayrık siteleri sırasında yüksek frekanslı periyodik faaliyet göstermek mümkün . . Hızlı rotorlar ile bölgenin en yüksek frekansta aktive eder ve genel aritmi sürücüler gibi rotor kaynaklanan 10,11 dalgaları fibrillatory iletim fenomen, fonksiyonel veya anatomik olarak kendi yolunda engellerle etkileşim 12 Haritalama endokardiyal yüzey posterior sol atrium (PLA), en yüksek rotor frekansı ile dalga dinamiği bölge AF izleme sağlar. Önemlisi, PLA intrakaviter kateter bazlı ablatif prosedürler hasta, 13 AF sol atrium iç dinamikleri eğitim alaka altını en başarılı sonlandırma AF bölgedir. Burada, insan paroksismal AF bazı özellikleri benzer akut streç indüklenen AF, bir koyun modeli açıklanmaktadır. Sol atrium epikardiyal haritalama, sert borescope AF bakım için en uygun bölge aktivasyon desenleri görselleştirmek için en doğrudan bir yaklaşımı temsil eden bir CCD kamera, c monte bir dual-channel kullanarak PLA endokardiyal haritalama ile tamamlanmaktadır.

Watch this Scientific Journal Video about High-Resolution Endocardial and Epicardial Optical Mapping in a Sheep Model of Stretch-Induced Atrial Fibrillation at JoVE.com

High-Resolution Endocardial and Epicardial Optical Mapping in a Sheep Model of Stretch-Induced Atrial Fibrillation

Bu rapor, streç bağlı atriyal fibrilasyon sırasında Langendorff perfüze koyun kalp bozulmamış sol atrium elektriksel uyarım eşzamanlı endokardiyal ve epikardiyal optik haritalama metodolojisi ve sonuçları ayrıntılı bir açıklamasını sağlar.

Stretch Bağlı Atriyal Fibrilasyon Koyun Modelinde Yüksek Çözünürlüklü Endokardial ve Epikardiyal Optik Haritalama

 Atrial fibrillation (AF) is a complex cardiac arrhythmia with high morbidity and mortality.1,2 It is the most common sustained cardiac rhythm disturbance ...

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Peripheral arterial disease (PAD) is a significant cause of morbidity resulting from chronic exposure to atherosclerotic risk factors. Patients suffering ...