Name: microfabricated post-array-detectors (mpads): an approach to isolate mechanical forces
Uploaded: 2007-10-01T12:10:27
Description: Watch this Scientific Journal Video about Microfabricated Post-Array-Detectors mPADs: an Approach to Isolate Mechanical Forces at JoVE.com

硅主模具制造 光刻技术 材料： <ul><li> 75毫米的硅晶片</li><li> SU - 8 2负光阻（Microchem，波士顿，MA） </li><li>氮气</li></ul>方法： <ol><li> 2个小时，脱水在120 ° C的硅晶片。 </li><li> 7分钟的紫外线臭氧治疗表面，以除去有机物。 </li><li>氮气吹表面，以去除微粒。 </li><li>应用SU - 8 2，覆盖面70％（约10毫升）75毫米晶圆。 </li><li>在2000年的20秒的转速旋转300转/ S加速。 <...

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	<li>Tan, J. L., Tien, J., Pirone, D., Gray, D. S., Chen, C. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cells+lying+on+a+bed+of+microneedles:+An+approach+to+isolate+mechanical+force.">Cells lying on a bed of microneedles: An approach to isolate mechanical force.</a> Proc Nat Acad Sci USA. 100, 1484-1489 (2003).</li><li>Sniadecki, N. J., Chen, C. S., Wang, Y. L., Discher, D. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Microfabricated+Silicone+Elastomeric+Post+Arrays+for+Measuring+Traction+Forces+of+Adherent+Cells.">Microfabricated Silicone Elastomeric Post Arrays for Measuring Traction Forces of Adherent Cells.</a> Methods in Cell Biology - Cell Mechanics. 83, 313-328 (2007).</li><li>Lemmon, C. A., Sniadecki, N. J., Ruiz, S. A., Tan, J. T., Romer, L. H., Chen, C. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Shear+Force+at+the+Cell-Matrix+Interface:+Enhanced+Analysis+For+Microfabricated+Post+Array+Detectors.">Shear Force at the Cell-Matrix Interface: Enhanced Analysis For Microfabricated Post Array Detectors.</a> Mechanics &amp; Chemistry of Biosystems. 2, 1-16 (2005).</li></ol>

microfabricated post-array-detectors (mpads): an approach to isolate mechanical forces

在这个视频中，我们将提出我们的方法来测量细胞牵引力使用微阵列的职位。通过肌球蛋白，肌动蛋白相互作用产生牵引力，并发挥着重要的生理作用。他们在发展过程中，使细胞从一个位置移动到下为了形成早期组织结构。牵引力量的帮助，在愈合过程。他们是必要的适当的伤口闭合或白细胞的迁移，并通过我们的身体爬行。癌转移或对肿瘤的血管生长的情况下，这些力量可以损害我们的健康。最常见的方法，通过它来研究细胞在体外已使用玻璃或聚苯乙烯盘。然而，承印物上的刚性，使得它无法测量细胞牵引力，并有相对较少的方法来研究牵引力。我们的实验室已开发出一种技术来克服这些限制。该方法是基于一个灵活的悬臂，刚度和大小规模，其中单个细胞遍布许多悬臂和转移的过程中垂直排列。我们使用的支柱是直径为3微米，10微米高，并定期在9微米的中心到中心间距阵列配置。但是，这些物理尺寸可以很容易地变化，以适应各种研究。我们开始用硅主，但最后的职位是由硅橡胶称为聚（二甲基硅氧烷），或硅橡胶。我们可以在显微镜下测量的挠度计算需要观察变形的牵引力量的大小和方向。我们呼吁这些基板微阵列后，探测器，或mPADs。在这里，我们将向您展示我们如何制造和使用mPADs以​​评估细胞收缩调制。

Watch this Scientific Journal Video about Microfabricated Post-Array-Detectors mPADs: an Approach to Isolate Mechanical Forces at JoVE.com

Microfabricated Post-Array-Detectors mPADs: an Approach to Isolate Mechanical Forces

在这段视频中，我们展示了如何制造和使用微加工后的阵列探测器（mPADs）评估细胞收缩的调制。

微阵列后探测器（mPADs）：机械力的方法来隔离

In this video, we will present our approach to measure cellular traction forces using a microfabricated array of posts.  Traction forces are generated ...

Research

JoVE Journal

Biology

Microfabricated Post-Array-Detectors (mPADs): an Approach to Isolate Mechanical Forces

Using Micro-Electro-Mechanical Systems (MEMS) to Develop Diagnostic Tools

Using Micro-Electro-Mechanical Systems MEMS to Develop Diagnostic Tools

使用微机电系统（MEMS）开发诊断工具

科研

生物学

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对急性机械刺激的网柄反应的评估

Chemotaxis, or migration up a gradient of a chemoattractant, is the best understood mode of directed migration. Studies using social amoeba Dictyostelium ...

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从兔模型中分离纯化滑液源性间充质干细胞的磁活化细胞分选策略

Mesenchymal stem cells (MSCs) are the main cell source for cell-based therapy. MSCs from articular cavity synovial fluid could potentially be used for ...

Protrusion Force Microscopy: A Method to Quantify Forces Developed by Cell Protrusions

凸出力显微镜: 一种量化细胞突起力的方法

In numerous biological contexts, animal cells need to interact physically with their environment by developing mechanical forces. Among these, traction ...

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In recent years, cerebral pericytes have become the focus of extensive research in vascular biology and pathology. The importance of pericytes in blood ...